Immobilization of enzymes on alginic acid–polyacrylamide copolymers

Kumaraswamy, M. D. K.; Rao, K. Panduranga; Joseph, K. T.; Santappa, M.

doi:10.1002/bit.260230815

Cited by 4 publications

(2 citation statements)

References 3 publications

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“…Synthetic polymers such as polyacrylamide (PAAm) or other synthetic polyacrylic polymer bearing the acylhydrazine moiety [242], or composites [241,243], can also be activated to bear active acyl azide as side-chains for binding enzymes. Direct preparation of polymers bearing acyl azide has been achieved by polymerization of unsaturated monomers bearing acyl azide functional groups, as illustrated in Scheme 3.56 [244].…”

Section: Polymers Bearing Acyl Azidementioning

confidence: 99%

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Covalent Enzyme Immobilization

Cao¹

2005

Carrier‐bound Immobilized Enzymes

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Section: Polymers Bearing Acyl Azidementioning

confidence: 99%

“…PAAm-ã-alginic acid activated by esterification and subsequent conversion to the acyl azide achieved payloads of 0.236 and 0.269 for papain and trypsin, respectively [241].…”

Section: Conversion To Aldehyde Functionalitymentioning

confidence: 99%

Covalent Enzyme Immobilization

Cao¹

2005

Carrier‐bound Immobilized Enzymes

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Entrapment of concanavalin A‐glycoenzyme complexes in calcium alginate gels

Husain

Iqbal

Saleemuddin

1985

Biotech & Bioengineering

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Glucose oxidase, invertase, and amyloglucosidase were entrapped in calcium alginate gels as concanavalin A complexes in order to prevent the leaching out of the enzymes from the porous matrix. The free as well as the gel-entrapped concanavalin A-glycoenzyme complexes exhibited a relatively high effectiveness factor, eta, indicating good accessibility to the substrates. Concanavalin A-invertase complex exhibited marked broadening of pH-activity and temperature-activity profiles and was highly resistant to temperature inactivation even after entrapment in the alginate beads. It was possible to entrap considerable quantities of invertase as concanavalin A complex in the beads without a marked decrease in eta. A column containing crosslinked concanavalin A-invertase complex entrapped in alginate beads retained the ability to completely hydrolyze 1M sucrose even after continuous operation for over four months.

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