Immobilization of Cellulase onto Core-Shell Magnetic Gold Nanoparticles Functionalized by Aspartic Acid and Determination of its Activity

Основные Виды Носителей Для Иммобилизации Целлюлазы

“…Добавление ионов металлов (например, меди) к аминофункциональным магнитным наночастиам позволяет улучшить иммобилизацию целлюлазы за счет сродства к металлу [15]. Авторы [15] использовали золотую оболочку вокруг магнитных наночастиц с последующей кремнеземной оболочкой и функционализацией с помощью полиэтиленгликоля и L-аспарагиновой кислоты для ковалентного присоединения целлюлазы.…”

Immobilized cellulase: Interactions between cellulase and nanostructured supports

“…The addition of metal ions (for example, copper) to aminofunctionalized magnetic LPS makes it possible to improve the immobilization of cellulase due to metal affinity [36]. For better protection of iron oxide nanoparticles Purakbar et al a gold shell around magnetic nanoparticles was used, followed by a silica shell and functionalization with PEG and L-aspartic acid for covalent addition of cellulase [37]. Another way to synthesize a magnetic nanobiocatalyst is realized by introducing magnetic nanoparticles into porous or polymer materials [38].…”

Upstream and Downstream Bioprocessing in Enzyme Technology

“…The optimization process focused on critical The covalent binding of enzymes to a support is carried out using a bifunctional chemical compound that binds to the chemical groups-such as the lysine groups-of the support and the amino acids on the enzyme's surface [18]. An example of covalent binding was the immobilization of the enzyme cellulase on functionalized core-shell magnetic gold nanoparticles [53]. The immobilization efficiency by proteins was 84%, and the activity by filter paper (FPase) was 0.78 mmol•mL −1 .…”