Immobilization of cellulase onto a recyclable thermo-responsive polymer as bioconjugate

Ding, Zhaoyang; Zheng, Xuexuan; Li, Sipeng; Cao, Xuejun

doi:10.1016/j.molcatb.2016.03.007

Cited by 31 publications

(27 citation statements)

References 33 publications

(39 reference statements)

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“…Such materials are characterized by a change in properties/behavior upon external stimuli [ 2 ], including light [ 3 ], temperature [ 4 , 5 ], electric field [ 6 ], and pH [ 7 ]. Among them, thermo-responsive materials have huge potential in biomedical applications due to their capability of phase transition above or below a certain temperature [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing

et al. 2018

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In this work, an aggregation-induced emission (AIE) molecule (tetraphenylethene derivative, TPE-COOH) was conjugated to elastin-like polypeptides (ELPs40) via an amide bond to form ELPs40-TPE. The successful synthesis of ELPs40-TPE was confirmed by Circular Dichroism spectroscopy, gel electrophoresis, UV-vis absorption, and fluorescence emission spectroscopy. ELPs40-TPE possessed both amphiphilicity and the features of an AIE, and the fluorescence intensity was dependent on the local temperature. The Hela cells imaging indicated that ELPs40-TPE has great potential for bio-imaging applications because of its advantages of high fluorescence intensity, good water-solubility, and remarkable biocompatibility.

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Section: Introductionmentioning

confidence: 99%

Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing

et al. 2018

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“…A sample cell with a 1 cm path length was used to measure the transmittance using 500 nm over a range of temperatures from 25 to 50 °C using a UV−Vis spectrophotometer (Shimadzu UV-3600 spectrophotometer, Shimadzu, Japan). The temperature at which the transmittance decreased to half of its initial value was taken as the LCST [ 34 ]. Fluorescence spectra (Fluorolg-4 spectrofluorometric) with a temperature controller were performed for the experiments.…”

Section: Methodsmentioning

confidence: 99%

Thermo-Responsive Fluorescent Polymers with Diverse LCSTs for Ratiometric Temperature Sensing through FRET

Ding¹,

Wang²,

Feng³

et al. 2018

Polymers

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Abstract:Temperature is a significant parameter to regulate biological reactions and functions inside cells. Sensing the intracellular temperature with a competent method is necessary to understand life science. In this work, an energy-transfer polymeric thermometer was designed for temperature sensing. The thermometer was prepared from two thermo-responsive polymers with different lower critical solution temperatures (LCSTs) of 31.1 • C and 48.6 • C, coupling with blue and red fluorescent molecules, respectively, developed for ratiometric temperature sensing based on the Förster resonance energy transfer (FRET) mechanism. The polymers were synthesized from two monomers, N-isopropylacrylamide (NIPA) and N-isopropylmethacrylamide (NIPmA), which provided different temperature responses. The fluorescent intensity of each polymer (peaked at 436 and 628 nm, respectively) decreased upon the heating of the polymer aqueous solution. While these two polymer aqueous solutions were mixed, the fluorescent intensity decrease at 436 nm and substantial fluorescence enhancement at 628 nm was observed with the increasing temperature due to FRET effect. The cell imaging of HeLa cells by these thermo-responsive polymers was explored. The difference of LCSTs resulting in ratiometric fluorescence change would have a potential impact on the various biomedical applications.

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“…The system proved to be favourable for the delivery of chemotherapeutic agents in the treatment of tumor, due to the prolonged drug release [106]. Bioconjugate of thermosensitive polymer and enzyme of celulase with the recycling capacity was obtained in the study of Ding et al [107]. First, a copolymer was synthesized through polymerization from methyl acrylate, MA, NIPMAM and N-methylolacrylamide, N-MAM, which subsequently carried out bioconjugation with celulase through the process of polymerization, PNMN-C.…”

Section: (1) (2018) 79-91mentioning

confidence: 99%

Hydrogels based on N-isopropylmethacrylamide and N-isopropylacrylamide

Urošević¹,

Nikolić²,

Ilić‐Stojanović³

et al. 2018

Adv Techol

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Hydrogels are three-dimensional polymer networks which have the capacity to retain a large quantity of water or biological fluids in the swollen state. Thermosensitive hydrogels have received special attention of reserachers since they represent a parameter which frequently changes in chemical, biological and physiological systems. Thermosensitive hydrogels have the critical solution temperature, i.e. they exhibit a substantial change in volume with the temperature change. Homopolymers poly(N-isopropylmethacrylamide) (poly(NIPMAM)) and poly(N-isopropylacrylamide) (poly(NIPAM)) are thermosensitive materials which have lately become the subject of intensive study. Monomer N-isopropylmethacrylamide, NIPMAM, enters into copolymerization with monomer N-isopropylacrylamide, NIPAM, in order to create a system with a phase transition temperature approximate to the human body temperature. In literature data there is available information on the synthesis and characterization of microgels, nanogels and copolymers based on NIPMAM and NIPAM. These thermosensitive polymer materials are used in controlled drug delivery and protein immobilization.

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Immobilization of cellulase onto a recyclable thermo-responsive polymer as bioconjugate

Cited by 31 publications

References 33 publications

Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing

Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing

Thermo-Responsive Fluorescent Polymers with Diverse LCSTs for Ratiometric Temperature Sensing through FRET

Hydrogels based on N-isopropylmethacrylamide and N-isopropylacrylamide

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