Immobilization of cells with nitrilase activity from a thermophilic bacterial strain

Kabaivanova, Lyudmila; Добрева, Е.; Dimitrov, Plamen; Эмануилова, Э.

doi:10.1007/s10295-004-0189-7

Cited by 28 publications

(11 citation statements)

References 13 publications

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“…However, the results presented allowed estimation of effective diffusion coefficients, which may be of help for designing a suitable reactor to lift the existing mass transfer limitations. Furthermore, efficient reusability of the biocatalyst up to ten batches was obtained by immobilization, and the high storage stability was al- so achieved, which can reduce fermentation workload [Kabaivanova et al, 2005]. The shift of optimal pH of biotransformation after immobilization can be explained by the abilities of cells to endure lower pH.…”

Section: Discussionmentioning

confidence: 97%

Biosynthesis of Iminodiacetic Acid from Iminodiacetonitrile by Immobilized Recombinant <i>Escherichia coli</i> Harboring Nitrilase

et al. 2012

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Iminodiacetic acid (IDA) is widely used as an intermediate in the manufacture of chelating agents, glyphosate herbicides and surfactants. In the current work, the fragment with the length of 1,110 bp encoding the Acidovorax facilis nitrilase was obtained. The recombinant nitrilase expressed in Escherichia coli BL21 (DE3) was successfully used in the production of IDA from iminodiacetonitrile. To improve the stability of operation, the recombinant cells were entrapped in polyvinyl alcohol (PVA) and sodium alginate (SA) copolymer. The maximum relative nitrilase activity with 98.1% was further observed at 1.0% SA, 8.0% PVA, 1.0% CaCl₂, and 5.0% wet cells, under conditions of 1.0% iminodiacetonitrile in distilled water and a temperature of 40°C, respectively. The entrapped cells facilitated easy separation and good recycling compared with free cells. Moreover, the immobilized cells showed good operation and storage stability. This report is the first to describe IDA preparation using immobilized recombinant E. coli harboring nitrilase.

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Section: Discussionmentioning

confidence: 97%

Biosynthesis of Iminodiacetic Acid from Iminodiacetonitrile by Immobilized Recombinant <i>Escherichia coli</i> Harboring Nitrilase

et al. 2012

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“…Only one thermophilic microorganism, Bacillus sp. UG-5B, has been reported to biotransform benzonitrile in an immobilized state with retention of 100% activity after eight reaction cycles (Kabaivanova et al 2005). We have described the immobilization of whole cells of Streptomyces sp.…”

Section: Discussionmentioning

confidence: 99%

Nitrilase-catalysed conversion of acrylonitrile by free and immobilized cells of Streptomyces sp.

et al. 2009

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The biotransformation of acrylonitrile was investigated using thermophilic nitrilase produced from a new isolate Streptomyces sp. MTCC 7546 in both the free and immobilized state. Under optimal conditions,the enzyme converts nitriles to acids without the formation of amides.The whole cells of the isolate were immobilized in agar-agar and the beads so formed were evaluated for 25 cycles at 50 degrees C. The enzyme showed a little loss of activity during reuse. Seventy-one per cent of 0.5 M acrylonitrile was converted to acid at 6 h of incubation at a very low density of immobilized cells, while 100% conversion was observed at 3 h by free cells.

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“…Entrapment seemed to be a better choice for immobilization [1,4,12,17], and immobilization of Alcaligenes faecalis ECU0401 cells in calcium alginate beads was used for further racemic mandelonitrile hydrolysis. To investigate the effect of recycling on the degree of mandelonitrile conversion, biotransformation reaction was carried out both with free cells and alginate-entrapped cells in batch mode.…”

Section: Efficient Biocatalyst Recyclingmentioning

confidence: 99%

Biocatalytic synthesis of (R)-(−)-mandelic acid from racemic mandelonitrile by cetyltrimethylammonium bromide-permeabilized cells of Alcaligenes faecalis ECU0401

Zhang

et al. 2010

J Ind Microbiol Biotechnol

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The nitrilase from Alcaligenes faecalis ECU0401 belongs to the category of arylacetonitrilase, which could hydrolyze 2-chloromandelonitrile, 3,4-dimethoxyphenylacetonitrile, mandelonitrile, and phenylacetonitrile into the corresponding arylacetic acids. To overcome the permeability barrier and prepare whole cell biocatalysts with high activities, permeabilization of Alcaligenes faecalis ECU0401 in relation to nitrilase activity was optimized by using cetyltrimethylammonium bromide (CTAB) as permeabilizing agent. The nitrilase activity from Alcaligenes faecalis ECU0401 increased 4.5-fold when the cells were permeabilized with 0.3% (w/v) CTAB for 20 min at 25 degrees C and pH 6.5. Consequently, almost all the mandelonitrile was consumed and converted to (R)-(-)-mandelic acid with greater than 99.9% enantiomeric excess (e.e.) by the CTAB-permeabilized cells. The permeability barrier has been significantly reduced in the hydrolysis of mandelonitrile by using CTAB-permeabilized cells and a dynamic resolution was successfully achieved, giving a 100% theoretical yield of (R)-(-)-mandelic acid. Efficient biocatalyst recycling was achieved as a result of cell immobilization in calcium alginate, with a product-to-biocatalyst ratio of 3.82 g (R)-(-)-mandelic acid g(-1) dry cell weight (dcw) cell after 20 cycles of repeated use.

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Immobilization of cells with nitrilase activity from a thermophilic bacterial strain

Cited by 28 publications

References 13 publications

Biosynthesis of Iminodiacetic Acid from Iminodiacetonitrile by Immobilized Recombinant <i>Escherichia coli</i> Harboring Nitrilase

Biosynthesis of Iminodiacetic Acid from Iminodiacetonitrile by Immobilized Recombinant <i>Escherichia coli</i> Harboring Nitrilase

Nitrilase-catalysed conversion of acrylonitrile by free and immobilized cells of Streptomyces sp.

Biocatalytic synthesis of (R)-(−)-mandelic acid from racemic mandelonitrile by cetyltrimethylammonium bromide-permeabilized cells of Alcaligenes faecalis ECU0401

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