Immobilization of Cells in Polysaccharide Gels

Yi, Yunyu; Neufeld, Ronald J.; Poncelet, Denis

doi:10.1201/9781420030822.ch39

Cited by 8 publications

(10 citation statements)

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“…Because of this stability, durability, wide usage, and very well-characterized properties, agarose could be advantageous, even though its gel is mechanically weaker than alginate or carrageenan gels. 43 Pioneering work on agarose for cell encapsulation was conducted by Iwata and colleagues. [25][26][27][28] Agarose microparticles can be produced by a simple one-step extrusion procedure 58 or by the water-in-oil emulsion technique.…”

Section: 44mentioning

confidence: 99%

Progress technology in microencapsulation methods for cell therapy

Rabanel

Banquy

Zouaoui

et al. 2009

Biotechnology Progress

122

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Cell encapsulation in microcapsules allows the in situ delivery of secreted proteins to treat different pathological conditions. Spherical microcapsules offer optimal surface-to-volume ratio for protein and nutrient diffusion, and thus, cell viability. This technology permits cell survival along with protein secretion activity upon appropriate host stimuli without the deleterious effects of immunosuppressant drugs. Microcapsules can be classified in 3 categories: matrix-core/shell microcapsules, liquid-core/shell microcapsules, and cells-core/shell microcapsules (or conformal coating). Many preparation techniques using natural or synthetic polymers as well as inorganic compounds have been reported. Matrix-core/shell microcapsules in which cells are hydrogel-embedded, exemplified by alginates capsule, is by far the most studied method. Numerous refinement of the technique have been proposed over the years such as better material characterization and purification, improvements in microbead generation methods, and new microbeads coating techniques. Other approaches, based on liquid-core capsules showed improved protein production and increased cell survival. But aside those more traditional techniques, new techniques are emerging in response to shortcomings of existing methods. More recently, direct cell aggregate coating have been proposed to minimize membrane thickness and implants size. Microcapsule performances are largely dictated by the physicochemical properties of the materials and the preparation techniques employed. Despite numerous promising pre-clinical results, at the present time each methods proposed need further improvements before reaching the clinical phase.

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Section: 44mentioning

confidence: 99%

Progress technology in microencapsulation methods for cell therapy

Rabanel

Banquy

Zouaoui

et al. 2009

Biotechnology Progress

122

View full text Add to dashboard Cite

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“…Another technique is to emulsify agarose in a warm fluid which is immiscible with the aqueous polysaccharide solution. Emulsion is produced which is then cooled to get microspheres 377. By casting in different containers, film or discs can be formed depending on the application.…”

Section: Seaweed Polysaccharidesmentioning

confidence: 99%

“…Beers are clarified with carrageenans, which complex and precipitate proteinaceous impurities. As with agarose and alginates, carrageenans are used for encapsulation 377. The behaviour observed depends directly on the composition of the systems in which carrageenans are incorporated.…”

Section: Seaweed Polysaccharidesmentioning

confidence: 99%

“…Carrageenan beads are obtained by extrusion of a solution into a KCl solution or by using an emulsion‐dispersion process 377. Different forms can be obtained based on thermal treatment and casting.…”

Section: Seaweed Polysaccharidesmentioning

confidence: 99%

“…Their behaviour depends directly on the DE. In some cases they are preferred to alginate because it is claimed that they are less sensitive to ions and chemical agents 377, 471. Stabilization of pectate beads was also obtained based on polyelectrolyte complex formation with polyethyleneimine followed by chemical crosslinkage with glutaraldehyde 472, 473.…”

Section: Other Plant Polysaccharidesmentioning

confidence: 99%

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Main properties and current applications of some polysaccharides as biomaterials

Rinaudo

2007

Polymer International

871

537

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This review concerns the applications of some polysaccharides in the domain of biomaterials and bioactive polymers. Natural polysaccharides from different sources have been studied for a long time, and their main properties are summarized in this paper; some of their derivatives obtained by chemical modification are also described. The main polysaccharides currently used in the biomedical and pharmaceutical domains are chitin and its derivative chitosan, hyaluronan and alginates. Alginates are well known for their property of forming a physical gel in the presence of divalent counterions (Ca, Ba, Sr) whereas carrageenans form a thermoreversible gel; these seaweed polysaccharides are mainly used to encapsulate different materials (cells, bacteria, fungi). Other promising systems are the electrostatic complexes formed when an anionic polysaccharide is mixed with a cationic polysaccharide (e.g. alginate/chitosan or hyaluronan/chitosan). An important development of the applications of polysaccharides can be predicted for the next few years in relation to their intrinsic properties such as biocompatibility and biodegradability in the human body for some of them; they are also renewable and have interesting physical properties (film‐forming, gelling and thickening properties). In addition, they are easily processed in different forms such as beads, films, capsules and fibres. Copyright © 2007 Society of Chemical Industry

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