Immobilization of cell-associated enzymes by entrapment in polymethacrylamide beads

Chiang, Chung-Jen; Hsiau, Liang-Tzung; Lee, Wen‐Chien

doi:10.1023/b:bite.0000034016.43050.22

Cited by 17 publications

(3 citation statements)

References 16 publications

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“…Encapsulation of an enzyme into a semipermeable polymer membrane is an inexpensive method of enzyme immobilization. [39][40][41] It decreases the leaching of the enzyme and improves its stability in the absence of covalent bonds between enzyme and polymer. The enzyme structure is oen unaltered as there is Pepsin, trypsin 21,22 Scheme 1 Reaction scheme for covalent immobilization of ADA on an APTSactivated silica matrix by using glutaraldehyde as the cross-linking agent.…”

Section: Encapsulationmentioning

confidence: 99%

Advances in immobilized enzyme microbioreactors in capillary electrophoresis

Iqbal

Müller

2013

Analyst

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Miniaturized bioanalytical systems are increasingly being used in the field of biochemical research. Immobilized enzyme microbioreactors in capillary electrophoresis (CE) have been constructed and used to fulfill the increasing demands for miniaturized bioanalytical systems. This manipulation permits low sample consumption, reduced costs, short analysis times and efficient analyses. This review provides an overview of the distinct characteristics of immobilized microbioreactors in CE. After an introduction on miniaturized microreactors, the various methods of enzyme immobilization will be discussed. The emphasis will be on two common constructions of microreactors in CE, i.e. CE-coupled microreactors, and microreactors directly integrated into the CE capillary. Such microbioreactors offer straightforward automation of reaction steps followed by separation in the same capillary.

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Section: Encapsulationmentioning

confidence: 99%

Advances in immobilized enzyme microbioreactors in capillary electrophoresis

Iqbal

Müller

2013

Analyst

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“…This is frequently done with sol-gels and hydrogels and is quite successful at immobilizing proteins on surfaces (17)(18)(19)(20). Traditional and electropolymerized polymers can also be employed for entrapping enzymes (21,22). Issues to consider with entrapment techniques are the chemical environment of the polymerization solution and whether it will denature the protein as well as the pore size and interconnectivity of the pores in the polymer to determine if transport of substrate and product can diffuse in and out of the polymer, but to ensure that the protein cannot diffuse out of the polymers.…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Introduction to the Field of Enzyme Immobilization and Stabilization

Moehlenbrock

Minteer

2010

Enzyme Stabilization and Immobilization

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Enzyme stabilization is important for any biomedical or industrial application of enzymes. In many applications, the goal is to provide extended active lifetime at normal environmental conditions with traditional substrates at low concentrations in buffered solutions. However, as enzymes are used for more and more applications, there is a desire to use them in extreme environmental conditions (i.e., high temperatures), in high substrate concentration, and in nontraditional solvent systems. This chapter introduces the topic of enzyme stabilization and the methods used for enzyme stabilization including enzyme immobilization.

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“…12 There are several techniques for enzyme immobilization including the well-established methods, physical adsorption of enzymes, covalent binding, and entrapment of enzymes in a growing polymer. [13][14][15] The sonochemical process was found to be a very efficient method for synthesizing and simultaneously depositing inorganic nanoparticles (NPs) on a wide variety of surfaces such as fabrics, 16 fibers and polymers. 17,18 Only recently, the sonochemical technique has been found to be very efficient in the formation of enzyme nanoparticles from their solution.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound coating of polydimethylsiloxanes with antimicrobial enzymes

et al. 2015

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There is an urgent need for antimicrobial functionalization of urinary catheters to prevent their colonization by microbes and biofilm formation. Here, the antimicrobial hydrogen peroxide (H 2 O 2 ) producing enzyme cellobiose dehydrogenase (CDH) was for the first time grafted onto polydimethylsiloxanes (PDMS) using an ultrasound assisted coating method. This resulted in the development of an effective in-situ continous H 2 O 2 producing system able to continually prevent microbial colonization and biofilm formation on catheters. This enzyme has an added advantage that it uses various oligosaccharides including expolysaccharides (an important part of the bioflim produced by the microbes while colonizing biomaterials) as electron donors to produce H 2 O 2 . Successful immobilization of active CDH nanoparticles on PDMS was confirmed by ESEM and AFM analysis as well as quantification of H 2 O 2 . Depending on the initial enzyme concentration, CDH-nanoparticles of varying sizes from 65±17nm to 93±17nm were created by the ultrasonic waves and subsequently deposited on the PDMS surface. PDMS sheets treated for 3 min produced 18 µM of H 2 O 2 within 2 hours which was sufficient to significantly reduce the amount of viable S. aureus cells as well as the total amount of biomass deposited on the surface. The ultrasound assisted coating of antimicrobial enzymes therefore provides an easy approach to immobilize enzymes and create a surface with antimicrobial properties.

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Immobilization of cell-associated enzymes by entrapment in polymethacrylamide beads

Cited by 17 publications

References 16 publications

Advances in immobilized enzyme microbioreactors in capillary electrophoresis

Advances in immobilized enzyme microbioreactors in capillary electrophoresis

Introduction to the Field of Enzyme Immobilization and Stabilization

Ultrasound coating of polydimethylsiloxanes with antimicrobial enzymes

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