Immobilization of carbonic anhydrase on polyethylenimine/dopamine codeposited membranes

Sun, Jing; Wang, Caihong; Wang, Yanzi; Ji, Shuxin; Liu, Wenfang

doi:10.1002/app.47784

Cited by 41 publications

(19 citation statements)

References 34 publications

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“…Some authors propose the treatment with glutaraldehyde of enzymes immobilized via ion exchange to prevent the enzyme release if high ionic strength or drastic pH values are used (e.g., hydrolysis of concentrated oils solutions, where the pH may decrease below 4 in the presence of high concentrations of free fatty acids) [23,[43][44][45].…”

Section: Treatment Of the Biocatalysts With Glutaraldehydementioning

confidence: 99%

Increasing the Enzyme Loading Capacity of Porous Supports by a Layer-by-Layer Immobilization Strategy Using PEI as Glue

et al. 2019

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A new strategy to increase the enzyme-loading capacity of porous supports was investigated. Lipase from Pseudomonas fluorescens (PFL) was immobilized on octyl-agarose (OA) beads and treated with polyethyleneimine (PEI). Then, PFL was immobilized on the previous PFL layer. Next, the biocatalyst was coated with PEI and a third layer of PFL was added. Sodium dodecyl sulfate polyacrylamide electrophoresis showed that the amount of PFL proportionally increased with each enzyme layer; however, the effects on biocatalyst activity were not as clear. Hydrolyzing 50 mM of triacetin at 25 °C, the activity of the three-layer biocatalyst was even lower than that of the bi-layer one; on the contrary its activity was higher when the activity was measured at 4 °C in the presence of 30% acetonitrile (that reduced the activity and thus the relevance of the substrate diffusion limitations). That is, the advantage of the multilayer formation depends on the specific activity of the enzyme and on the diffusion limitations of the substrate. When octyl agarose (OA)-PFL-PEI-PFL preparation was treated with glutaraldehyde, the activity was reduced, although the enzyme stability increased and the immobilization of the last PFL layer offered results similar to the one obtained using the three-layer preparation without glutaraldehyde modification (90%).

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Section: Treatment Of the Biocatalysts With Glutaraldehydementioning

confidence: 99%

Increasing the Enzyme Loading Capacity of Porous Supports by a Layer-by-Layer Immobilization Strategy Using PEI as Glue

et al. 2019

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“…Figure 6 b proves that PDA forms on the surface of Fe 3 O 4 nanoparticles. In Figure 6 c, a strong absorption peak was observed at 1650–1600 cm −1 , corresponding to the Michael addition between PDA and PEI and the C=N and C=O structures formed by Schiff base reaction [ 39 , 40 ]. A strong absorption peak was observed at 3000–2800 cm −1 and 1450 cm −1 , belonging to the stretching vibration peak of -CH 2 - in PEI and the in-plane bending vibration peak, respectively.…”

Section: Resultsmentioning

confidence: 99%

Fe3O4@PDA@PEI Core-Shell Microspheres as a Novel Magnetic Sorbent for the Rapid and Broad-Spectrum Separation of Bacteria in Liquid Phase

Zhang¹,

Du²,

Wu³

et al. 2022

Materials

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Bacterial infection is a significant cause of morbidity and mortality to humans worldwide. Thus, a method for nonspecific, sensitive, and rapid enrichment of such bacteria is essential for bacteria detection and treatment. This study demonstrates a self-made core-shell Fe3O4@Polydopamine@Polyethyleneimine magnetic beads (Fe3O4@PDA@PEI MBs) with a high density positive charge-based magnetic separation scheme for the broad-spectrum rapid enrichment of microorganisms in the liquid phase. MBs with a high-density positive charge have a strong electrostatic attraction to most microorganisms in nature. Our scheme is as follows: (1) wrapping dopamine (DA) on the iron oxide through self-polymerization and wrapping PEI on the outermost shell layer in a mode of crosslinking with the PDA; (2) subsequently, the Fe3O4@PDA@PEI MBs were used to concentrate microorganisms from the sample solution; (3) performing magnetic separation and calculating the adsorption efficiency. The as-prepared Fe3O4@PDA@PEI MBs composite was carefully characterized by zeta potential analysis, Value stream-mapping (VSM), transmission electron microscopy (TEM), and Fourier transforms infrared spectrometry (FT-IR). In this study, both gram-positive and gram-negative bacteria could be captured in three minutes through electrostatic interaction. Furthermore, the adsorption efficiency on gram-negative (>98%) is higher than that on gram-positive (>95%), allowing for a simple, rapid assay to enrich organisms in resource-limited settings.

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“…Usually this method is applied to immobilize enzymes in solid supports for its reuse, as observed in the work of Sun et al . The authors studied immobilization of carbonic anhydrase on polymeric support of poly(vinylidene fluoride) (PVDF) and polyethylene (PE) previously modified via codeposition of polyethyleneimine (PEI) and dopamine (DA) for CO 2 capture, reducing emission of this gas.…”

Section: Resultsmentioning

confidence: 99%

Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

Silva

Nascimento

et al. 2019

J of Applied Polymer Sci

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The use of macroporous monolithic matrices in the purification of biocompounds is constantly growing and developing. In this work, the objective was to optimize the quantity of N-acetyl-D-glucosamine (D-GlcNAc) immobilized on the surface of macroporous polymeric cryogels for capture of lectins from less clarified solutions. Surface response methodology was applied and it was observed that the immobilization temperature of the glutaraldehyde (GLU) and the D-GlcNAc concentration influenced the amount of sugar immobilized. The matrices produced with 1.1% of allyl glycidyl ether were functionalized by GLU. Optimal maximum condition was obtained with mean value of 160.39 AE 26.38 mg of D-GlcNAc immobilized per gram of dry cryogel. Characterization analyses of the matrices showed that the activation process was effective, maintaining the macroporous structure and physical characteristics. The adsorbents produced were tested for capture of lectins from a crude protein solution of barley. At tested conditions, adsorbent capture around 11% of protein in solution but reduce the hemaglutinating capacity in 40%, demonstrating its selectivity. The cryogels functionalized with D-GlcNAc present potential for use in capture compounds by affinity with carbohydrates, such as lectins.

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Immobilization of carbonic anhydrase on polyethylenimine/dopamine codeposited membranes

Cited by 41 publications

References 34 publications

Increasing the Enzyme Loading Capacity of Porous Supports by a Layer-by-Layer Immobilization Strategy Using PEI as Glue

Increasing the Enzyme Loading Capacity of Porous Supports by a Layer-by-Layer Immobilization Strategy Using PEI as Glue

Fe3O4@PDA@PEI Core-Shell Microspheres as a Novel Magnetic Sorbent for the Rapid and Broad-Spectrum Separation of Bacteria in Liquid Phase

Enhancements in sugar immobilization in polymeric macroporous matrices for affinity capture

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