Immobilization of Candida rugosa lipase on electrospun cellulose nanofiber membrane

Huang, Xiaojun; Chen, Pengcheng; Huang, Feng; Ou, Yang; Chen, Mingrui; Xu, Zhi‐Kang

doi:10.1016/j.molcatb.2011.02.010

Cited by 182 publications

(60 citation statements)

References 36 publications

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“…Electrospun nanofibers are promising carriers to overcome these issues [44,[60][61][62][63]. Nanofiber membranes have a large surface area for enzyme loading and high porosity for efficient substrate diffusion.…”

Section: Electrospun Nanofibers-based Lipase Immobilizationmentioning

confidence: 99%

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Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Kim

Lee

2018

Catalysts

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Abstract:The cost of biodiesel production relies on feedstock cost. Edible oil is unfavorable as a biodiesel feedstock because of its expensive price. Thus, non-edible crop oil, waste oil, and microalgae oil have been considered as alternative resources. Non-edible crop oil and waste cooking oil are more suitable for enzymatic transesterification because they include a large amount of free fatty acids. Recently, enzymes have been integrated with nanomaterials as immobilization carriers. Nanomaterials can increase biocatalytic efficiency. The development of a nano-immobilized enzyme is one of the key factors for cost-effective biodiesel production. This paper presents the technology development of nanomaterials, including nanoparticles (magnetic and non-magnetic), carbon nanotubes, and nanofibers, and their application to the nano-immobilization of biocatalysts. The current status of biodiesel production using a variety of nano-immobilized lipase is also discussed.

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Section: Electrospun Nanofibers-based Lipase Immobilizationmentioning

confidence: 99%

“…The efficiency of the biocatalysis increased because the K m value of the immobilized lipase decreased. Huang et al [61] reported the development of immobilized CRL on an electrospun cellulose nanofiber membrane via covalent binding. The nanofiber was made by electrospun cellulose acetate.…”

Section: Electrospun Nanofibers-based Lipase Immobilizationmentioning

confidence: 99%

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Kim

Lee

2018

Catalysts

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“…10 A nanoszálba ágyazás során nem-ionos felületaktív anyagokat és organoszilánokat alkalmaztunk additívként, melyek befolyásolhatják a rögzített enzim biokatalitikus képességeit. Szén nanocsövek különbözõ felületi funkcionalizálása révén olyan hordozók elõállítását terveztük, melyek képesek lehetnek enzimek stabil és hatékony rögzítésére.…”

Section: Bevezetésunclassified

Enzimrögzítési módszerek nanostruktúrált rendszerekben

Weiser¹

2018

MKF

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“…The lipase activity was determined using a method previously reported [35]. Briefly, the reaction was started by putting immobilized lipase preparation in the reaction mixture composed of 1.0 mL ethanol containing 14.4 mM p-nitrophenyl palmitate and 1.0 mL PBS (0.05 M, pH 7.0).…”

Section: Assay Of Lipase Activitymentioning

confidence: 99%

Adsorption and Activity of Lipase on Polyphosphazene-Modified Polypropylene Membrane Surface

et al. 2016

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Abstract:In this work, poly(n-butylamino)(allylamino)phosphazene (PBAP) was synthesized and tethered on polypropylene microporous membrane (PPMM) with the aim of offering a biocompatible and, at the same time, moderately hydrophobic microenvironment to lipase for the first time. Lipase from Candida rugosa was used and the influence of membrane surface conditions on the activities of immobilized lipases was evaluated. Water contact angle measurement as well as field emission scanning electron microscopy were used to characterize the morphology of the modified membranes. The results showed an improvement in the adsorption capacity (26.0 mg/m 2 ) and activity retention (68.2%) of the immobilized lipases on the PBAP-modified PPMM. Moreover, the lipases immobilized on the modified PPMM showed better thermal and pH stability.

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Immobilization of Candida rugosa lipase on electrospun cellulose nanofiber membrane

Cited by 182 publications

References 36 publications

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

Enzimrögzítési módszerek nanostruktúrált rendszerekben

Adsorption and Activity of Lipase on Polyphosphazene-Modified Polypropylene Membrane Surface

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