Immobilization of Candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters

Zheng, Mingming; Lu, Yong; Liu, Dong; Guo, Pingmei; Deng, Qianchun; Li, Wenlin; Feng, Yun; Huang, Fenghong

doi:10.1016/j.biortech.2011.11.128

Cited by 73 publications

(40 citation statements)

References 25 publications

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“…Hence, the hydrophobic interaction is more important for the adsorption of lipase on the polyamine microsphere in comparison with that of the other proteins tested. Lipase prefers to adsorb on hydrophobic supports, involving in the adsorption of the hydrophobic areas surrounding the active center [29–31]. During the crosslinking of PEI with glutaraldehyde, the hydrocarbon segments in the molecular chains of PEI and glutaraldehyde intertwined to form hydrophobic patches on the polyamine microsphere.…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Polyamine Microsphere and Its Adsorption for Protein

Wang

Liu

Nie

et al. 2012

IJMS

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A novel polyamine microsphere, prepared from the water-in-oil emulsion of polyethylenimine, was characterized. The investigation of scanning electron microscopy showed that the polyamine microsphere is a regular ball with a smooth surface. The diameter distribution of the microsphere is 0.37–4.29 μm. The isoelectric point of the microsphere is 10.6. The microsphere can adsorb proteins through the co-effect of electrostatic and hydrophobic interactions. Among the proteins tested, the highest value of adsorption of microsphere, 127.8 mg·g−1 microsphere, was obtained with lipase. In comparison with other proteins, the hydrophobic force is more important in promoting the adsorption of lipase. The microsphere can preferentially adsorb lipase from an even mixture of proteins. The optimum temperature and pH for the selective adsorption of lipase by the microsphere was 35 °C and pH 7.0.

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Section: Resultsmentioning

confidence: 99%

Characterization of a Polyamine Microsphere and Its Adsorption for Protein

Wang

Liu

Nie

et al. 2012

IJMS

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“…Such a configuration permits an immobilization of the greater amounts of the enzymes at only insubstantial growth of cost of the carrier production. Silicas of smaller surface areas obtained mainly in the processes of hydrolysis and condensation of tetraalkoxysilanes (Grabicka and Jaroniec 2010;Fornera and Bauer 2012;Zheng et al 2012) were also used for the enzyme immobilization.…”

Section: Inorganic Carriersmentioning

confidence: 99%

“…On the contrary, the presence of -SH or -NH 2 groups compatible with the enzyme functional groups, facilitates generation of carrier-modifier-enzyme interactions. Other trialkylsilanes used as carrier modifiers include n-octyltriethoxysilane (Zheng et al 2012), phenyltrimethoxysilane, vinyltrimethoxysilane (Hartono et al 2010) and [3-(trimethoxysilyl)propyl] octadecyl dimethyl ammonium chloride (Tran et al 2012). The attachment of the most common surface modifying agents to silica particles is shown in Fig.…”

Section: Surface Modifying Agentsmentioning

confidence: 99%

“…By contrast, among inorganic carriers widely used there are different silicas, such as mesoporous silicas (Gustafsson et al 2012), e.g. SBA-15 or MSU-H (Yu and Fang 2013), vesicular silica (Wu et al 2012), fumed silica (Kramer et al 2010), silanized silica (Zheng et al 2012), silica sol-gel film (Lee et al 2010) and commercial silica-based products Celite (Brem et al 2011) and Celite 545 (Kumar and Kanwar 2011;see Fig. 5).…”

Section: Immobilized Enzymesmentioning

confidence: 99%

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Enzyme immobilization by adsorption: a review

2014

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Endowed with unparalleled high catalytic activity and selectivity, enzymes offer enormous potential as catalysts in practical applications. These applications, however, are seriously hampered by enzymes' low thermal and chemical stabilities. One way to improve these stabilities is the enzyme immobilization. Among various tested methods of this process that make use of different enzyme-carrier interactions, immobilization by adsorption on solid carriers has appeared most common. According to these findings, in this review we present a comparative analysis of the literature reports on the recent trends in the immobilization of the enzymes by adsorption. This thorough study was prepared in order to provide a deeper understanding of the process. Both carriers, carrier modifiers and procedures developed for effective adsorption of the enzymes are discussed. The review may thus be helpful in choosing the right adsorption scheme for a given enzyme to achieve the improvement of its stability and activity for a specific application.

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“…For instance, to bind enzymes to a solid support, microporous and macroporous materials are widely used, such as electrospun polymer fibers and some mixedmode silica particles (Soumanou et al, 2012;Zheng et al, 2012). Especially, entrapment for enzymes immobilization in membrane reactors, magnetic nanoparticles and hollow polyelectrolyte microspheres are very popular and practice (Lopez et al, 2004;Crestini et al, 2010;Bellino and Regazzoni, 2011).…”

Section: Introductionmentioning

confidence: 99%