Immobilization of Candida rugosa lipase on eco-friendly supports by spouted-bed technology: Use in the synthesis of isoamyl caprylate

Abstract

Candida rugosa lipase (LCR) was immobilized on low-cost supports (by-products) and dried using a spouted-bed system. The yields of immobilized derivatives were in the range 61.5–78.7%. Lipase immobilized on rice husk showed the best results, presenting 94.1% of the original activity, followed by sugarcane bagasse (90.3%) and green coconut fiber (87.3%). Moisture content in the obtained powders varied between 4.7 and 5.6% and the water activities were in the range 0.21–0.35. Among all the tested biocatalysts for aroma production the lipase immobilized on rice husk showed the highest activity towards the formation of isoamyl caprylate (62.40 g.L-1). Keywords: Spouted bed dryer; Enzyme dehydration; Enzyme immobilization; Enzyme stabilization; Aroma production.