Immobilization of Candida antarctica lipase B on ILs modified CNTs with different chain lengths: Regulation of substrate tunnel “Leucine gating”

Lu, Zeping; Chen, Mei; Jin, Tongtong; Nian, Binbin; Hu, Yi

doi:10.1016/j.ijbiomac.2023.125894

Cited by 5 publications

(2 citation statements)

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“…Enzymes are excellent and multifunctional biocatalysts with high potential for applications in various fields such as biotechnology. − Enzymatic reaction has high selectivity and specificity and is usually carried out under mild temperature, pressure, and pH conditions, which is a green chemistry synthesis method. − In addition, enzymes are obtained through bioengineering methods such as microbial fermentation, which is sustainable. − However, practically, free enzymes are not only difficult to recover and reuse but also less stable and active within organic solvents and under extreme temperature and pH conditions, which limits their applications. , Study of enzyme immobilization is usually applied to deal with the problems . Many materials are used for enzyme immobilization, including polymer carriers, inorganic amorphous silica, carbon nanotubes, and silica nanoparticles. − These studies have to some extent improved the problem of easy aggregation and recovery of free enzymes. However, these methods directly fix the exposed enzymes on the surface of the material, have not addressed the stability of enzymes under extreme conditions (temperature and pH), and are relatively complex.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, they can only be used in stirred reactions and not in continuous fluid catalytic reactions, resulting in low reaction efficiencies. ,− Therefore, the application of enzyme immobilization in continuous microfluidic reactors has aroused great interest. For example, wall-coated reactors and packed-bed reactors that can achieve continuous production and long-term reuse have been successfully developed, but they still have some limitations: low amount of fixed catalyst, high cost, cumbersome packaging operations, and low mass transfer efficiency. − Using monolithic materials may be a good choice.…”

Section: Introductionmentioning

confidence: 99%

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Continuous Fluid Catalytic Reactor Constructed Based on In Situ Growth of MOF Immobilized Enzyme on Ordered Silk Fabric

Wang,

Chen,

Zhou

et al. 2024

ACS Sustainable Chem. Eng.

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Enzyme catalysis applications are limited because of their poor stability under extreme conditions. Using metal−organic frameworks (MOFs) as enzyme immobilization carriers efficiently solves this problem. However, MOFs easily agglomerate, are difficult to recover as a crystalline powder, and can only be used under continuous stirring, resulting in low reaction efficiencies. The use of immobilized enzymes for continuous fluid catalysis to improve catalytic efficiency remains challenging. At the same time, the spatial division of most immobilized enzyme porous materials is disordered, which leads to poor mass transfer efficiency and consequently low reaction rates. In this context, we want to construct a new type of reactor that solves the problem of enzyme stability by encapsulating enzymes in MOFs and then using low-cost and easily available silk fabric materials as immobilized carriers to load MOF immobilized enzymes. The ordered structure of silk fabric enhances substrate mass transfer, thereby improving the catalytic efficiency of the reactor. By comparing the mass transfer of a continuous fluid catalytic reactor with ordered silk fiber immobilized enzyme (OSCR) with that of a continuous fluid catalytic reactor with disordered silk fiber immobilized enzyme (DSCR) using crystal violet adsorption and catalytic process simulations, we demonstrated that the reaction efficiency in ordered spaces can be improved through mass transfer within each partition space. Finally, the reactor was used in the biotransformation of polydatin and demonstrated a high conversion of 98%, which was much higher than that of DSCR. This study revealed the enormous potential of ordered structure enzyme reactors in continuous fluid catalysis.

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