Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide

Santos, José Cleiton Sousa dos; Bonazza, Horacio; Matos, Leonardo José Brandão Lima de; Carneiro, E. A.; Barbosa, Oveimar; Fernández-Lafuente, Roberto; Gonçalves, Luciana Rocha Barros; Ana, Hosiberto Batista de Sant’; Santiago-Aguiar, Rílvia Saraiva de

doi:10.1016/j.btre.2017.02.003

Cited by 78 publications

(35 citation statements)

References 85 publications

(109 reference statements)

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“…However, when EF was used as a feruloyl donor, FG yield and EF conversion increased with the reaction progress. EF conversion was 88.6 2.3 at 24 h, which was higher than FA as the feruloyl donor 30 . These results can be attributed to the higher melting and lower solubility of FA 174 than EF 64 in this reaction system, which results in a large mass transfer limitation in the system with FA as the feruloyl donor.…”

Section: Enzyme Screeningmentioning

confidence: 78%

Hydrophilic Glyceryl Ferulates Preparation Catalyzed by Free Lipase B from Candida antartica

Yao

Sun

2020

J. Oleo Sci.

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Introduction Ferulic acid FA is also known as 4-hydroxy-3methoxy cinnamic acid, is ubiquitously found in some plants, and has UV-absorbing, antioxidant, free radical scavenging and antitumor activities 1 4. However, the low hydrophilicity of FA has limited its application in pharmaceutical, cosmetic and other fields 5 7. To overcome this problem, modifications of FA using hydrophilic groups for example, glycerol, hydrophilic aliphatic alcohols and monosaccharides has attracted attention 8 13. Glyceryl ferulate FG is a popular hydrophilic FA derivative 14 17. And FG exhibited good UV adsorbing properties at 280-360 nm with a λ max at 322 nm. In previous reports, chemical catalysts and enzymes have been used for FG preparation. The presence of phenolic hydroxyl makes FA easy to oxidize and sensitive to heat. Therefore, compared with chemical catalysts, enzymes have been popular catalysts for the preparation of FA derivatives, due to their mild reaction conditions 14, 16, 18 .

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Section: Enzyme Screeningmentioning

confidence: 78%

Hydrophilic Glyceryl Ferulates Preparation Catalyzed by Free Lipase B from Candida antartica

Yao

Sun

2020

J. Oleo Sci.

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“…The horseradish peroxidase on surface modified mesoporous activated carbon combined with scCO 2 enhances the diffusivity and helps remove the phenol in hydrogen peroxide [101,102]. Lipase has been used by this method to increase the conversion rate in many studies, such as C. Antarctica lipase making the conversion rate up to 1.16 times higher [103]. In addition, enzymes immobilized into MOF supports combined with scCO 2 methods can also obtain higher conversion rates.…”

Section: Enzyme Immobilization In Supercritical Fluidsmentioning

confidence: 99%

Recent Developments in Carriers and Non-Aqueous Solvents for Enzyme Immobilization

2019

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Immobilization techniques are generally based on reusing enzymes in industrial applications to reduce costs and improve enzyme properties. These techniques have been developing for decades, and many methods for immobilizing enzymes have been designed. To find a better immobilization method, it is necessary to review the recently developed methods and have a clear overview of the advantages and limitations of each method. This review introduces the recently reported immobilization methods and discusses the improvements in enzyme properties by different methods. Among the techniques to improve enzyme properties, metal–organic frameworks, which have diverse structures, abundant organic ligands and metal nodes, offer a promising platform.

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“…Periodically, samples were withdrawn, and the activity was measured using PNPA. The deactivation constant and half-life of each immobilized derivative were calculated according to the model of Sadana and Henley [55] using Microcal Origin version 8.1 [29]. …”

Section: Effect Of Phmentioning

confidence: 99%

“…The objective of this study was to analyze the effects of the encapsulation of a model enzyme on a novel support formed of chitosan and polyphosphate. As the model enzyme, we had selected the lipase B from Candida antarctica (CALB), which is one of the most widely reported enzymes in the literature [29,45]. This biocatalyst was utilized to produce benzyl acetate via a transesterification reaction.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Benzyl Acetate Catalyzed by Lipase Immobilized in Nontoxic Chitosan-Polyphosphate Beads

Silva¹,

Melo²,

Lemos³

et al. 2017

Preprint

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Enzymes serve as biocatalysts for innumerable important reactions; however, their application has limitations, which could be overcome by using appropriate immobilization strategies. Here, a new support for immobilizing enzymes is proposed. This hybrid organicinorganic support is composed of chitosan-a natural, nontoxic, biodegradable, and edible biopolymer-and sodium polyphosphate, which was the inorganic component. Lipase B from Candida antarctica (CALB) was immobilized in microspheres by encapsulation using these polymers. The characterization of the composites (by infrared spectroscopy, thermogravimetric analysis, and confocal Raman microscopy) confirmed the hybrid nature of the support, whose external part consisted of polyphosphate and core was composed of chitosan. The immobilized enzyme had the following advantages: possibility of enzyme reuse, easy biocatalyst recovery, increased resistance to variations in temperature (activity declined from 60°C and the enzyme was inactivated at 80°C), and increased catalytic activity in the transesterification reactions. The encapsulated enzymes were utilized as biocatalysts for transesterification reactions to produce the compound responsible for the aroma of jasmine.

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Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide

Abstract: Graphical abstract

Cited by 78 publications

References 85 publications

Hydrophilic Glyceryl Ferulates Preparation Catalyzed by Free Lipase B from <i>Candida antar</i><i>tica</i>

Hydrophilic Glyceryl Ferulates Preparation Catalyzed by Free Lipase B from <i>Candida antar</i><i>tica</i>

Recent Developments in Carriers and Non-Aqueous Solvents for Enzyme Immobilization

Synthesis of Benzyl Acetate Catalyzed by Lipase Immobilized in Nontoxic Chitosan-Polyphosphate Beads

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