Immobilization of biotinylated bacteriophages on biosensor surfaces

Gervais, Luc; Gel, Murat; Allain, B.; Tolba, Mona; Brovko, Lubov; Zourob, Mohammed; Mandeville, Rosemonde; Griffiths, Mansel W.; Evoy, Stéphane

doi:10.1016/j.snb.2007.03.007

Cited by 123 publications

(86 citation statements)

References 23 publications

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“…Thus, R ct variation due to bacteriophages binding to the host bacteria is somewhat different from other bioreceptors in that at the beginning R ct increases due to bacterial binding hindering the redox reaction at the WE surface, while later it decreases due to cell lyses allowing the release of cell ionic material. Bacteriophages have been used as immobilizing bioreceptors in biosensors for different bacterial species (Shabani et al, 2008;Gervais et al, 2007;Tolba et al, 2012), as well as for an unconventional approach to IM where bacteriophages are integrated into the culture broth, and the monitored variation of electrolyte resistance is mainly due to conductivity increase induced by cell lyses (and not only due to bacterial metabolism) (Mortari et al, 2015). According to the authors, this approach results in much better performances with a detection limit of 1 CFU mL −1 or lower and response time less than 1 h.…”

Section: Impedance Biosensorsmentioning

confidence: 99%

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Grossi

Riccò

2017

J. Sens. Sens. Syst.

296

160

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Abstract. Electrical impedance spectroscopy (EIS), in which a sinusoidal test voltage or current is applied to the sample under test to measure its impedance over a suitable frequency range, is a powerful technique to investigate the electrical properties of a large variety of materials. In practice, the measured impedance spectra, usually fitted with an equivalent electrical model, represent an electrical fingerprint of the sample providing an insight into its properties and behavior. EIS is used in a broad range of applications as a quick and easily automated technique to characterize solid, liquid, semiliquid, organic as well as inorganic materials. This paper presents an updated review of EIS main implementations and applications.

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Section: Impedance Biosensorsmentioning

confidence: 99%

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Grossi

Riccò

2017

J. Sens. Sens. Syst.

296

160

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“…Bacteriophage was immobilized on the surface of gold modified with streptavidin, and pictures were taken as described previously (13).…”

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confidence: 99%

Oriented Immobilization of Bacteriophages for Biosensor Applications

Tolba

Minikh

Brovko

et al. 2010

Appl Environ Microbiol

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115

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A method was developed for oriented immobilization of bacteriophage T4 through introduction of specific binding ligands into the phage head using a phage display technique. Fusion of the biotin carboxyl carrier protein gene (bccp) or the cellulose binding module gene (cbm) with the small outer capsid protein gene (soc) of T4 resulted in expression of the respective ligand on the phage head. Recombinant bacteriophages were characterized in terms of infectivity. It was shown that both recombinant phages retain their lytic activity and host range. However, phage head modification resulted in a decreased burst size and an increased latent period. The efficiency of bacteriophage immobilization with streptavidin-coated magnetic beads and cellulosebased materials was investigated. It was shown that recombinant bacteriophages form specific and strong bonds with their respective solid support and are able to specifically capture and infect the host bacterium. Thus, the use of immobilized BCCP-T4 bacteriophage for an Escherichia coli B assay using a phage multiplication approach and real-time PCR allowed detection of as few as 800 cells within 2 h.

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“…These results agree well with those obtained with lytic phages immobilized to glass disk surfaces by a covalent binding technique 27 . The capability of bound lytic phages to capture host bacteria were also demonstrated by using a biotinylated phage immobilization technique 30 . In contrast, a very simple physical adsorption method for attaching properly oriented phages to solid surfaces has been utilized 31 .…”

Section: Representative Resultsmentioning

confidence: 99%

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Guntupalli

Sorokulova

Olsen

et al. 2013

JoVE

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A structurally transformed lytic bacteriophage having a broad host range of Staphylococcus aureus strains and a penicillin-binding protein (PBP 2a) antibody conjugated latex beads have been utilized to create a biosensor designed for discrimination of methicillin resistant (MRSA) and sensitive (MSSA) S. aureus species 1,2 . The lytic phages have been converted into phage spheroids by contact with water-chloroform interface. Phage spheroid monolayers have been moved onto a biosensor surface by Langmuir-Blodgett (LB) technique 3 . The created biosensors have been examined by a quartz crystal microbalance with dissipation tracking (QCM-D) to evaluate bacteria-phage interactions. Bacteria-spheroid interactions led to reduced resonance frequency and a rise in dissipation energy for both MRSA and MSSA strains. After the bacterial binding, these sensors have been further exposed to the penicillin-binding protein antibody latex beads. Sensors analyzed with MRSA responded to PBP 2a antibody beads; although sensors inspected with MSSA gave no response. This experimental distinction determines an unambiguous discrimination between methicillin resistant and sensitive S. aureus strains. Equally bound and unbound bacteriophages suppress bacterial growth on surfaces and in water suspensions. Once lytic phages are changed into spheroids, they retain their strong lytic activity and show high bacterial capture capability. The phage and phage spheroids can be utilized for testing and sterilization of antibiotic resistant microorganisms. Other applications may include use in bacteriophage therapy and antimicrobial surfaces. Video LinkThe video component of this article can be found at

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Immobilization of biotinylated bacteriophages on biosensor surfaces

Cited by 123 publications

References 23 publications

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Oriented Immobilization of Bacteriophages for Biosensor Applications

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

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