Immobilization of bacterial proteases on water-solved polymer by means of electron beam

Gonchar, A.M.; Auslender, V.L.

doi:10.1016/0969-806x(95)00468-d

Cited by 6 publications

(2 citation statements)

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“…In addition, imprecise terminology impedes a clear overview. In earlier publications, terms such as “radiation immobilization” [ 54 ] or “electron beam immobilization” [ 55 ] were used; however, they actually refer to radiation-induced polymerization of a hydrogel [ 56 ], although it was already suspected that “radiation itself may lead to chemical bonding between the biomaterials and the polymer matrix” [ 40 ]. Therefore, we propose a new term, radiation-induced graft immobilization (RIGI), in analogy to the well-known RIGP, emphasizing the direct and covalent attachment of non-reactive compounds.…”

Section: Introductionmentioning

confidence: 99%

Radiation-Induced Graft Immobilization (RIGI): Covalent Binding of Non-Vinyl Compounds on Polymer Membranes

et al. 2021

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Radiation-induced graft immobilization (RIGI) is a novel method for the covalent binding of substances on polymeric materials without the use of additional chemicals. In contrast to the well-known radiation-induced graft polymerization (RIGP), RIGI can use non-vinyl compounds such as small and large functional molecules, hydrophilic polymers, or even enzymes. In a one-step electron-beam-based process, immobilization can be performed in a clean, fast, and continuous operation mode, as required for industrial applications. This study proposes a reaction mechanism using polyvinylidene fluoride (PVDF) and two small model molecules, glycine and taurine, in aqueous solution. Covalent coupling of single molecules is achieved by radical recombination and alkene addition reactions, with water radiolysis playing a crucial role in the formation of reactive solute species. Hydroxyl radicals contribute mainly to the immobilization, while solvated electrons and hydrogen radicals play a minor role. Release of fluoride is mainly induced by direct ionization of the polymer and supported by water. Hydrophobic chains attached to cations appear to enhance the covalent attachment of solutes to the polymer surface. Computational work is complemented by experimental studies, including X-ray photoelectron spectroscopy (XPS) and fluoride high-performance ion chromatography (HPIC).

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Section: Introductionmentioning

confidence: 99%

Radiation-Induced Graft Immobilization (RIGI): Covalent Binding of Non-Vinyl Compounds on Polymer Membranes

et al. 2021

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“…в растворе белок-ПЭГ [5]. Несмотря на то, что данный метод модификации не получил достаточно широкого распространения, иммобилизация, индуцированная потоком ускоренных электронов, была ранее упомянута в ряде публикаций [22,38,43]. Фармакологические свойства веществ, иммобилизированных с помощью технологии электронно-лучевого синтеза.…”

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Technology of electron-beam synthesis as a perspective direction in development of immobilized interferons for oral use (literature report)

Kinsht¹,

Мадонов²,

Ластовецкий³

et al. 2017

Journal of New Medical Technologies. eJournal

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Центральный НИИ организации и информатизации здравоохранения, ул. Добролюбова, 11, Москва, 127254, Россия *** Тульский государственный университет, пр-т Ленина, д. 92, Тула, 300028, Россия **** ФГБНУ «Томский национальный исследовательский медицинский центр Российской академии наук», НИИ фармакологии и регенеративной медицины им. Е.Д. Гольдберга, пр. Ленина, 3, г. Томск, 634028, Россия Аннотация. В обзоре представлены современные сведения о применении интерферонов в терапии заболеваний пищеварительной системы, фармакологических свойствах иммобилизированных на инертных носителях биологически активных веществ, технологии электронно-лучевого синтеза, а также фармакологических свойствах белков, иммобилизированных с помощью электронно-лучевой технологии.В настоящее время представляется перспективным применение в практической медицине пегилированных белковых молекул, обладающих биологической активностью. У иммобилизированных на полимерных носителях препаратов изменяются не только фармакокинетические параметры, конъюгация молекул с полиэиленоксидом может также изменять фармакодинамические свойства полученных веществ. Химическая иммобилизация белковых веществ в части случаев с успехом может быть заменена технологией электронно-лучевой иммобилизации, значительно снижающей себестоимость лекарственных препаратов. В обзоре представлены различия фармакологических свойств иммобилизированных биологически активных веществ белковой природы от свойств исходных белков. Решение задачи, связанной с биодоступностью белковых препаратов при пероральном применении, успешно может быть осуществлено с помощью использования технологии электронно-лучевого синтеза. Применение этой технологии для создания препаратов на основе интерферона может обеспечить адресную доставку молекул интерферона в органы пищеварительной системы при пероральном приеме.Ключевые слова: интерферон, интерферон α2-b, интерферон λ, иммобилизация, энтеровирусная инфекция, гепатит C.Abstract. The review presents modern information on the use of interferons in the therapy of diseases of the digestive system, the pharmacological properties of biologically active substances immobilized on inert carriers, the technology of electron-beam synthesis, as well as the pharmacological properties of proteins immobilized by electron beam technology.At present, it seems promising to use pegylated biological active protein molecules in practical medicine. Immobilized on polymeric carriers drugs change not only the pharmacokinetic parameters, since the conjugation of molecules with polyethylene oxide can also change the pharmacodynamic properties of the obtained substances. In some cases, the chemical immobilization of protein substances can be successfully replaced by the technology of electron beam immobilization, which significantly reduces the cost of medicines. The review presents the differences in the pharmacological properties of immobilized biologically active substances of the ВЕСТНИК НОВЫХ МЕДИЦИНСКИХ ТЕХНОЛОГИЙ, электронный журнал -2017 -N 3 JOURNAL OF NEW MEDICAL TECHNO...

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Use of High Power Electron Beam Radiation for Treatment of Municipal and Industrial Wastes

Auslender

1999

The Modern Problems of Electrostatics With Applications in Environment Protection

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Immobilization of bacterial proteases on water-solved polymer by means of electron beam

Cited by 6 publications

References 4 publications

Radiation-Induced Graft Immobilization (RIGI): Covalent Binding of Non-Vinyl Compounds on Polymer Membranes

Radiation-Induced Graft Immobilization (RIGI): Covalent Binding of Non-Vinyl Compounds on Polymer Membranes

Technology of electron-beam synthesis as a perspective direction in development of immobilized interferons for oral use (literature report)

Use of High Power Electron Beam Radiation for Treatment of Municipal and Industrial Wastes

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