Immobilization of Bacillus firmus strain 37 in inorganic matrix for cyclodextrin production

Moriwaki, Cristiane; Pelissari, Franciele Maria; Gonçalves, Regina Aparecida Correia; Gonçalves, José Eduardo; Matioli, Graciette

doi:10.1016/j.molcatb.2007.07.003

Cited by 27 publications

(37 citation statements)

References 34 publications

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“…For the micrographs, a scanning electron microscope with an accelerating voltage of 10 kV was used. The samples were placed on the surface of a conductive double sided tape and coated with gold [2].…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

“…CDs are cyclic maltooligosaccharides composed of 6 -60 glucose units linked by α-1, 4-glycosidic bonds. They have the ability to form a variety of inclusion complexes with many organic and inorganic compounds, which is of interest for pharmaceutical, food, cosmetic, textile and agrochemical industries [2] [5].…”

Section: Introductionmentioning

confidence: 99%

“…As CGTase produces preferentially α-, β-and/or γ-CD they are called as α-, β-and/or γ-CGTase. A CGTase that produces only one type of CD is not known, but a CGTase which produces a high proportion of one type of cyclodextrin is industrially favorable because it reduces the cost of the purifying process [2] [6] [7].…”

Section: Introductionmentioning

confidence: 99%

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Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Silva¹,

Bieli²,

Valarini³

et al. 2018

ACES

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The process described in the present work uses air supplementation in a fluidized bed reactor containing Bacillus firmus strain 37 immobilized on active bovine bone charcoal, to produce by batch fermentation the enzyme CGTase (cyclomaltodextrin-glucanotransferase). Three different aeration rates were evaluated. The maximum CGTase activity was achieved after 120 hours of fermentation with aeration rate of 2 vvm and was equal to 2.48 U/mL. When 0.5 and 1 vvm were used the enzymatic activities achieved 1.1 and 0.57 U/mL, respectively. Bovine bone charcoal was characterized in terms of surface area, pore size and volume. To the best of our knowledge, the immobilization of microorganism cells in bovine bone charcoal for CGTase production has not been reported in the literature. Our results showed that fluidized bed reactor allows retaining high concentration of biomass, improving biomass-substrate contact and operation at low residence times, which resulted in improved enzyme production. Therefore, the process as proposed has great potential for industrial development.

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Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Silva¹,

Bieli²,

Valarini³

et al. 2018

ACES

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“…To evaluate the immobilization of L. edodes in loofa sponges, SEM was performed according to the procedure described by Moriwaki et al (2007) with some adjustments. The immobilized cells were placed in 2.5 % glutaraldehyde solution for 24 h. After this period, the matrices containing the immobilized cells were washed in solutions of 30, 50, 70, 90, and 100 % ethanol.…”

Section: Assessment Of Immobilization Using Scanning Electron Microscmentioning

confidence: 99%

Biosorption potential of synthetic dyes by heat-inactivated and live Lentinus edodes CCB-42 immobilized in loofa sponges

Gimenez

Ruiz

Caetano

et al. 2014

World J Microbiol Biotechnol

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Lentinus edodes CCB-42 was immobilized in loofa sponges and applied to the biosorption of the synthetic dyes congo red, bordeaux red and methyl violet. Live immobilized microorganisms achieved average decolorations of congo red, bordeaux red and methyl violet of 97.8, 99.7 and 90.6 %, respectively. The loofa sponge was the support and the coadjuvant promoting dye adsorption. The biosorption conditions were optimized for each dye, yielding 30 °C, pH 5.0 and a 12 h reaction time for congo red; 25 °C, pH 3.0 and 36 h for bordeaux red; and 25 °C, pH 8.0 and 24 h for methyl violet. Operational stability was evaluated over five consecutive cycles, with both bordeaux red and congo red exhibiting decolorations above 90 %, while the decoloration of methyl violet decreased after the third cycle. In the sixth month of storage, congo red, bordeaux red and methyl violet had decolorations of 93.1, 79.4 and 73.8 %, respectively. Biosorption process best fit the pseudo-second-order kinetic and Freundlich isotherm models. Maximum biosorption capacity of heat-treated L. edodes immobilized in loofa sponge was determined as 143.678, 500.00 and 381.679 mg/g for congo red, bordeaux red and methyl violet, respectively. Treatment with immobilized L. edodes reduced the phytotoxicity of the medium containing dyes. FT-Raman experiments suggested the occurrence of interactions between loofa sponge fibers, L. edodes and dye. L. edodes CCB-42 immobilized in loofa sponges represents a promising new mode of treatment of industrial effluents.

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“…Using the cell immobilization method, increased protein excretion with reduction in cell lysis can be achieved. The advantages for the use of immobilized cells include the preservation of plasmid-bearing cells, decreased risk of contamination and protection of the cells from environmental stresses [1][2][3] . Therefore, the cell immobilization could reduce the occurrence of cell lysis.…”

Section: Introductionmentioning

confidence: 99%

Effect of the cross linkers’ concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase)

CheMan

Illias

Sulaiman

et al. 2017

Chem. Eng. Res. Bull.

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Abstract:The excretion of recombinant proteins into the culture medium is a preferred approach over cytoplasmic expression because of the high specific activity and ease of purification. However, cell lysis is one of a major problem during the excretion of recombinant protein due to the pressure build up in the periplasmic space through overproduction of the expressed recombinant protein. Cell immobilization is a promising solution for the improvement of protein excretion with reduction in cell lysis. In this study, the effect of cross linkers concentrations on the process of cell immobilization for the cyclodextringlucanotransferase (CGTase) excretion and cell viability were investigated. The hollow fiber membrane was treated using different concentrations (0.3, 0.67 and 1%) of cross linkers namely glutaraldehyde (GA), poly-L-lysine (PLL) and polyethylenimine (PEI). The optimum concentration of cross linkers was found to be 0.3% with the high excretion of CGTase. Interestingly, the immobilized cell on the non-treated hollow fiber membrane showed up to 15% increment of CGTase excretion with 55% reduction of cell lysis, as compared to the immobilized cells on the treated hollow fiber membrane. It could be concluded that, the low concentration of cross linkers exhibited the highest CGTase excretion. Moreover, the non-treated hollow fiber membrane is a promising approach for attachment of cells to the membrane without requiring treatment with any chemicals for the production of recombinant enzyme.

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Immobilization of Bacillus firmus strain 37 in inorganic matrix for cyclodextrin production

Cited by 27 publications

References 34 publications

Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Biosorption potential of synthetic dyes by heat-inactivated and live Lentinus edodes CCB-42 immobilized in loofa sponges

Effect of the cross linkers’ concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase)

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Immobilization of Bacillus firmus strain 37 in inorganic matrix for cyclodextrin production

Cited by 27 publications

References 34 publications

Bovine Bone Charcoal as Support Material for Immobilization of &lt;em&gt;Bacillus firmus&lt;/em&gt; Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Bovine Bone Charcoal as Support Material for Immobilization of &lt;em&gt;Bacillus firmus&lt;/em&gt; Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Biosorption potential of synthetic dyes by heat-inactivated and live Lentinus edodes CCB-42 immobilized in loofa sponges

Effect of the cross linkers’ concentration on the immobilization of recombinant Escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (CGTase)

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Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed