Immobilization of Alkaline Phosphatase onto Chitosan Nanoparticles: a Novel Therapeutic Approach in Bone Tissue Engineering

Jafary, Fariba; Vaezifar, Sedigheh

doi:10.1007/s12668-021-00886-7

Cited by 3 publications

(6 citation statements)

References 26 publications

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“…With the degradation of MALPNs, ALP, calcium ions, and phosphate ions will be released. ALP is biocompatibility and can be taken in as nutrients; , meanwhile, the released calcium ions and phosphate ions from CaP and MALPNs can promote the proliferation and differentiation of osteoblastic lineage cells by activating CaMKII-, AMPK-, ERK1/2-, and PI3K/Akt-related pathways. , However, the promoting effect is significantly determined by the concentration. Calcium ions and phosphate ions with higher concentration may induce cell injury by inducing mitochondrial swelling, as well as upregulating the reactive oxygen species (ROS) and autophagy. , Since BMSCs proliferate better when the MALPNs are added at a concentration of 100 μg mL –1 , the concentration of 100 μg mL –1 is selected to investigate the osteogenic differentiation behavior of MALPNs.…”

Section: Resultsmentioning

confidence: 99%

“…The role of ALP in biomineralization had been preliminarily explored via the preparation of amorphous CaP composites (MALPNs) under the regulation of ALP in a chemically and biologically similar microenvironment to the bone tissue, that is, DMEM. Although ALP has been widely utilized as an enzyme to induce in situ mineralization, − the ALP-incorporated biominerals have not been addressed the deserved attention to date. The MALPNs promote the osteogenic differentiation of BMSCs and endow the fractured bone tissues with better ability to regeneration.…”

Section: Resultsmentioning

confidence: 99%

“…With the degradation of MALPNs, ALP, calcium ions, and phosphate ions will be released. ALP is biocompatibility and can be taken in as nutrients; 19,21 meanwhile, the released calcium ions and phosphate ions from CaP and MALPNs can promote the proliferation and differentiation of osteoblastic lineage cells by activating CaMKII-, AMPK-, ERK1/2-, and PI3K/Akt-related pathways. 29,46 However, the promoting effect is significantly determined by the concentration.…”

Section: Preparation and Characterization Of The Malpn/ Gelma Hybrid ...mentioning

confidence: 99%

“…There have been several reports about the controlled release system of proteins for type 2 diabetes therapy , and cancer management based on biomimetic mineralization . ALP has been used for bone regeneration due to the enzymatic function, which could promote the hydrolysis of calcium glycerophosphate and further contribute to the formation of hydroxyapatite, − while to the best of our knowledge, there is few report about the mineralization strategy for the preparation of mineralized ALP nanoparticles (MALPNs).…”

Section: Introductionmentioning

confidence: 99%

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Mineralized Enzyme-Based Biomaterials with Superior Bioactivities for Bone Regeneration

Zhou

Fan

Jiang

et al. 2022

ACS Appl. Mater. Interfaces

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The formation and metabolic balance of bone tissue is a controllable process of biomineralization, which is regulated by various cells, biomolecules, and ions. Enzyme molecules play an important role in this process, and alkaline phosphatase (ALP) is one of the most critical factors. In this study, inspired by the process of bone biomineralization, a biomimetic strategy is achieved for the preparation of mineralized ALP nanoparticles (MALPNs), by taking advantages of the unique reaction between ALP and calcium ions in Dulbecco's modified Eagle's medium. Benefiting from the mild biomineralization reaction, the MALPN system highly maintains the activity of ALP. Furthermore, the in vitro studies show that the MALPN system significantly enhances the proliferation of bone marrow mesenchymal stem cells and upregulates their osteogenic differentiation. When evaluated as synthetic graft materials for bone regeneration, the MALPN-incorporated gelatin methacryloyl graft shows excellent mechanical properties, a sustained release profile of ALP, and high biocompatibility and efficacy in guiding bone regeneration and vascularization for criticalsized rat calvarial defect. Moreover, we also demonstrate that the biomimetic mineralization strategy can be adopted for other proteins such as acid phosphatase, bovine serum albumin, fibrinogen, and gelatin, suggesting its universality for constructing mineralized protein-/enzyme-based bioactive materials for the application of tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Preparation and Characterization Of The Malpn/ Gelma Hybrid ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mineralized Enzyme-Based Biomaterials with Superior Bioactivities for Bone Regeneration

Zhou

Fan

Jiang

et al. 2022

ACS Appl. Mater. Interfaces

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“…This work proved that chitosan nanoparticles can be used as an effective component in bone tissue therapy. Simultaneous use with alkaline transferase increases the expression of genes that are associated with the mineralization and diffusion of osteoblasts [ 116 ].…”

Section: Main Applications Of Biodegradable Polymeric Nanoparticlesmentioning

confidence: 99%

Biopolymeric Nanoparticles–Multifunctional Materials of the Future

et al. 2022

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Nanotechnology plays an important role in biological research, especially in the development of delivery systems with lower toxicity and greater efficiency. These include not only metallic nanoparticles, but also biopolymeric nanoparticles. Biopolymeric nanoparticles (BPNs) are mainly developed for their provision of several advantages, such as biocompatibility, biodegradability, and minimal toxicity, in addition to the general advantages of nanoparticles. Therefore, given that biopolymers are biodegradable, natural, and environmentally friendly, they have attracted great attention due to their multiple applications in biomedicine, such as drug delivery, antibacterial activity, etc. This review on biopolymeric nanoparticles highlights their various synthesis methods, such as the ionic gelation method, nanoprecipitation method, and microemulsion method. In addition, the review also covers the applications of biodegradable polymeric nanoparticles in different areas—especially in the pharmaceutical, biomedical, and agricultural domains. In conclusion, the present review highlights recent advances in the synthesis and applications of biopolymeric nanoparticles and presents both fundamental and applied aspects that can be used for further development in the field of biopolymeric nanoparticles.

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Recent advances in nano‐scaffolds for tissue engineering applications: Toward natural therapeutics

Bakhshayesh

Saghebasl

Asadi

et al. 2023

WIREs Nanomed Nanobiotechnol

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Among the promising methods for repairing or replacing tissue defects in the human body and the hottest research topics in medical science today are regenerative medicine and tissue engineering. On the other hand, nanotechnology has been expanded into different areas of regenerative medicine and tissue engineering due to its essential benefits in improving performance in various fields. Nanotechnology, a helpful strategy in tissue engineering, offers new solutions to unsolved problems. Especially considering the excellent physicochemical properties of nanoscale structures, their application in regenerative medicine has been gradually developed, and a lot of research has been conducted in this field. In this regard, various nanoscale structures, including nanofibers, nanosheets, nanofilms, nano‐clays, hollow spheres, and different nanoparticles, have been developed to advance nanotechnology strategies with tissue repair goals. Here, we comprehensively review the application of the mentioned nanostructures in constructing nanocomposite scaffolds for regenerative medicine and tissue engineering.This article is categorized under: Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement Diagnostic Tools > Biosensing

show abstract

Immobilization of Alkaline Phosphatase onto Chitosan Nanoparticles: a Novel Therapeutic Approach in Bone Tissue Engineering

Cited by 3 publications

References 26 publications

Mineralized Enzyme-Based Biomaterials with Superior Bioactivities for Bone Regeneration

Mineralized Enzyme-Based Biomaterials with Superior Bioactivities for Bone Regeneration

Biopolymeric Nanoparticles–Multifunctional Materials of the Future

Recent advances in nano‐scaffolds for tissue engineering applications: Toward natural therapeutics

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