Immobilization of Alcohol Dehydrogenase in membrane: Fouling mechanism at different enzyme concentration

Abstract

Fouling induced enzyme immobilization on polymer membrane with controlled variables can retain enzyme stability and performance. In this study, the effect of enzyme concentration towards the fouling-induced enzyme immobilization was investigated. Alcohol dehydrogenase (ADH) was used as model enzyme to be immobilized on poly(ether) sulfone (PES) membrane at different concentration (0.05, 0.10 and 0.20 g/L). The transmembrane pressure and pH were fixed at 2 bar and pH 7 respectively during the process takes place. The result shows that higher enzyme concentration gives higher loading efficiency with 83.95%. Hermia’s model predicted that standard and intermediate blocking dominating the fouling mechanism at all enzyme concentration tested.

A Review on the Design and Performance of Enzyme-Aided Catalysis of Carbon Dioxide in Membrane, Electrochemical Cell and Photocatalytic Reactors

“…Luo and co-workers introduced a membrane reactor setup which immobilized the three dehydrogenase enzymes, simultaneously or separately, in flat-sheet polymeric membranes (Figure 5) by simple pressure-driven filtration (i.e., by directing membrane fouling formation), without any addition of organic solvent [17]. Using this technique, the immobilization procedure is simple and facile [44][45][46], enzyme denaturation is minimized during immobilization, maximum enzyme loading is obtained without enzyme leakage during operation [47], and the product could be removed immediately from the enzyme active site in order to decrease product inhibition. From this research, it was found that co-immobilization did not improve methanol production compared with sequential immobilization because of the trade-off between the mitigation of product inhibition and low substrate concentration for the adjacent enzymes.…”

Effect of pH on membrane fouling during alcohol dehydrogenase immobilization in PES membrane

“…The existence of salts can adjust electrostatic protein-protein interactions and produce the effect of shielding charge and dampening out of intermolecular protein interactions (Chan & Chen, 2001;She et al, 2009). The membrane fouling commonly compromises the performance of the membrane in terms of separation efficiency and permeate flux (Ismail et al, 2020;. The main types of fouling membranes are physical adsorption, pore blocking of membrane, gel/cake formation and biofouling which are caused by complex interactions between membranes and foulants such as hydrophobic or electrostatic adsorption, particle deposition or aggregation, hydrogen bonding and bio-affinity (Guo et al, 2012;Sassolas et al, 2012).…”