Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography

Moravcová, Dana; Planeta, Josef; King, Alistair W. T.; Wiedmer, Susanne Κ.

doi:10.1016/j.chroma.2018.04.012

Cited by 20 publications

(6 citation statements)

References 28 publications

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“…ILs also have good thermal stability and are tasteless, and can be easily recycled [ 14 ]. Furthermore, the physical and chemical properties of ILs vary widely depending on their chemical structure [ 15 ], and because of numerous possibilities of ILs structures by cation and anion selection, they are widely used in organic biocatalytic syntheses, clean fuel production, and in other applications [ 16 ]. However, several studies have shown that ILs can threaten human health and exert adverse environmental effects [ 17 ], and their toxicity varies with their structure and physical and chemical properties [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Total Triterpenoid Extraction from Inonotus Obliquus Using Ionic Liquids and Separation of Potential Lactate Dehydrogenase Inhibitors via Ultrafiltration High-Speed Countercurrent Chromatography

et al. 2021

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Extracts of the fungus Inonotus obliquus exhibit cytotoxic properties against different cancers; hence, this fungal species has been extensively studied. This study aimed to extract total triterpenoids from Inonotus obliquus using ionic liquids (ILs) and separate potential lactate dehydrogenase (LDH) inhibitors via ultrafiltration (UF)-high-speed countercurrent chromatography (HSCCC). Total triterpenoids from Inonotus obliquus were extracted by performing a single-factor experiment and employing a central composite design via ultrasonic-assisted extraction (UAE) and heat-assisted extraction (HAE). The extract was composed of 1-butyl-3-methylimidazolium bromide as the IL and methanol as the dispersant. Ultrafiltration-liquid chromatography (UF-LC) was used to rapidly scan the LDH inhibitors and betulin and lanosterol were identified as potential inhibitors. To obtain these target compounds, betulin and lanosterol with the purities of 95.9% and 97.8% were isolated from HSCCC within 120 min. Their structures were identified using several techniques, among which IL-HAE was fast and effective. This study reports the extraction of triterpenoids from Inonotus obliquus by IL for the first time. Collectively, the findings demonstrate that UF-LC is an effective tool for screening potential LDH inhibitors from crude extracts of I. obliquus and may help to identify bioactive substances against myocardial infarction, whereas high-purity compounds can be separated via UF-HSCCC.

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Section: Introductionmentioning

confidence: 99%

Total Triterpenoid Extraction from Inonotus Obliquus Using Ionic Liquids and Separation of Potential Lactate Dehydrogenase Inhibitors via Ultrafiltration High-Speed Countercurrent Chromatography

et al. 2021

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“…The resulting ISEC curves are depicted in Figure 1 together with the values of the total ( ε T ), the external (interstitial, flow‐through, macropore) ( ε 0 ), and the internal (mesopore) ( ε i ) porosities. A capillary monolithic column prepared using TMOS [36] and particle packed column XBridge were also evaluated for comparison purposes.…”

Section: Resultsmentioning

confidence: 99%

“…The poly(octadecyl‐POSS‐PETA) monolithic column with an i.d. of 100 μm show better efficiency for non‐retained compound uracil ( N /m = 168 100, Figure 2), at linear mobile phase velocity of 1 mm/s corresponding to flow rate of 500 nl/min, than the rigid but brittle monolith prepared from tetrafunctional alkoxide (TMOS column; HILIC conditions, N /m = 123 900 for toluene – non‐retained compound [36]).…”

Section: Resultsmentioning

confidence: 99%

Fabrication of monolithic capillary columns with inner diameter 50–530 μm employing a mixture of pentaerythritol tetraacrylate and polyhedral oligomeric silsesquioxane‐methacrylate as crosslinkers

Planeta

Moravcová

Karásek

et al. 2022

J of Separation Science

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Highly crosslinked monolithic capillary columns with inner diameters in the range of 50-530 μm were prepared by radical polymerization of pentaerythritol tetraacrylate, polyhedral oligomeric silsesquioxane-methacrylate, and n-octadecyl methacrylate in the presence of methanol, dodecyl alcohol, and polyethylene glycol lauryl ether. Columns were evaluated by inverse sizeexclusion chromatography employing a set of polystyrene standards of narrow molecular-size distribution and by scanning electron microscopy. Chromatographic performance under reversed-phase conditions was also evaluated.The combination of two effective crosslinkers as pentaerythritol tetraacrylate and polyhedral oligomeric silsesquioxane-methacrylate in the polymerization mixture allows for the preparation of robust and efficient monolithic capillary columns within a fairly wide range of internal diameters.

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“…For example, a corannulene‐immobilized silica monolith was fabricated and applied in the analysis of aromatic analytes by cLC, indicating that the concave surface of corannulene has strong CH‐π interaction with a planar molecule (coronene) . Similarly, a silica monolithic column was modified by a self‐synthesized phosphonium‐based ionic liquid via 3‐(trimethoxysilyl)propyl methacrylate (γ‐MAPS), which was then used for separation of pyrimidine bases and nucleosides in HILIC and exhibited high efficiencies ranging from 98 000 to 174 000 plates m −1 …”

Section: Basic Strategy For Designing Monolithic Materialsmentioning

confidence: 99%

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Wang

et al. 2019

Advanced Materials

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High‐performance liquid chromatography integrated with tandem mass spectrometry (HPLC–MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the “heart” of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow‐through pores, are regarded as an alternative to particle‐packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next‐generation separation materials for high‐throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown.

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Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography

Cited by 20 publications

References 28 publications

Total Triterpenoid Extraction from Inonotus Obliquus Using Ionic Liquids and Separation of Potential Lactate Dehydrogenase Inhibitors via Ultrafiltration High-Speed Countercurrent Chromatography

Total Triterpenoid Extraction from Inonotus Obliquus Using Ionic Liquids and Separation of Potential Lactate Dehydrogenase Inhibitors via Ultrafiltration High-Speed Countercurrent Chromatography

Fabrication of monolithic capillary columns with inner diameter 50–530 μm employing a mixture of pentaerythritol tetraacrylate and polyhedral oligomeric silsesquioxane‐methacrylate as crosslinkers

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

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