Immobilization of A. oryzae β-galactosidase on Silica Nanoparticles: Development of an Effective Biosensor for Determination of Lactose in Milk Whey

Goel, Anchal; Sinha, Rajeshwari; Khare, Sunil Kumar

doi:10.1007/978-981-10-5275-0_1

Cited by 2 publications

(3 citation statements)

References 43 publications

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“…Immobilized enzyme showed higher stability over native enzyme, retaining 50% of initial activity after 6 h. The silica NPs−β-gal conjugates were also reused 14 cycles with preservation 50% of activity after 9 cycles. 119 Benival et al obtained 71% of immobilized β-galactosidase activity after 13 cycles, and it slightly decreased after 14 and 15 cycles. They also observed the temperature optimum range increase from 30 to 37 °C to 35−42 °C for free and immobilized enzymes, respectively.…”

Section: ■ Carrier Materialsmentioning

confidence: 99%

“…To determine β-galactosidase activity, 2-nitrophenyl-β- d -galactopyranoside was used as a substrate. Immobilized enzyme showed higher stability over native enzyme, retaining 50% of initial activity after 6 h. The silica NPs−β-gal conjugates were also reused 14 cycles with preservation 50% of activity after 9 cycles . Benival et al obtained 71% of immobilized β-galactosidase activity after 13 cycles, and it slightly decreased after 14 and 15 cycles.…”

Section: Inorganic Carriersmentioning

confidence: 99%

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Application of Immobilized Enzymes in Food Industry

Yushkova

Nazarova

Matyuhina

et al. 2019

J. Agric. Food Chem.

140

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Enzymes are macromolecular biocatalysts, widely used in food industry. In applications, enzymes are often immobilized on inert and insoluble carriers, which increase their efficiency due to multiple reusability. The properties of immobilized enzymes depend on the immobilization method and the carrier type. The choice of the carrier usually concerns the biocompatibility, chemical and thermal stability, insolubility under reaction conditions, capability of easy regeneration and reusability, as well as cost efficiency. In this review, we provide an overview of various carriers for enzyme immobilization, with the primary focus on food industry.

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Section: ■ Carrier Materialsmentioning

confidence: 99%

Section: Inorganic Carriersmentioning

confidence: 99%

Application of Immobilized Enzymes in Food Industry

Yushkova

Nazarova

Matyuhina

et al. 2019

J. Agric. Food Chem.

140

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“…It exhibited improved performance under high thermal denaturation conditions and retained 50% enzyme activity even after eleventh cycle reuse [45]. The utility of this nanobiocatalyst was investigated for immobilizing β-galactosidase from other source to convert lactose from whey and milk [46]. The developed nanomatrix showed 94% immobilization efficiency.…”

Section: Immobilization Of β-Galactosidase On Surface Modified Nanoparticlesmentioning

confidence: 99%

Overviewing the Application of β-Galactosidase “Immobilized on Nanoparticles” in Dairy Industries

Alshanberi

Al-Shaeri

Ansari³

2021

Braz. arch. biol. technol.

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Owing to the excellent catalytic potential, β-galactosidase (EC: 3.2.1.23) has been exploited as an important industrial enzyme for obtaining galactooligosaccharides (GOS) and lactose-free products in dairy industries. Moreover, novel technologies have been implemented in the recent past for preparing and modifying nanoparticles (NPs) for immobilizing therapeutically and industrially important enzymes. Nanoparticles based enzyme immobilization (NBEI) offered more stability and robustness to the enzymes due to their fixed conformation and hence extend their applications in broader areas. A quick overview of the results exhibited greater activity for the enzymes immobilized on NPs as compared to enzyme immobilized on 2-D matrices. Based on these findings, this review was aimed to emphasize the recent development achieved for immobilizing β-galactosidase on NPs with their specific utilization in obtaining dairy products. These studies includes β-galactosidases from various sources that were immobilized on various NPs for hydrolyzing lactose in batch and continuous reactors, and for the production of GOS in biotechnology industries. NBEI of β-galactosidase offered profound stability for transporting substrate and product for enzymatic reactions, apart from cost effective advantage due to reusable nature of immobilized enzyme. HIGHLIGHTS Nanoparticles (NPs) exhibited greater performance of immobilized enzyme. They influence the sensitivity, selectivity, loading, mobility and stability of enzymes. β-galactosidase immobilized on NPs retained greater activity against various physical and chemical denaturants. Alshanberi, A.M.; et al.

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Immobilization of A. oryzae β-galactosidase on Silica Nanoparticles: Development of an Effective Biosensor for Determination of Lactose in Milk Whey

Cited by 2 publications

References 43 publications

Application of Immobilized Enzymes in Food Industry

Application of Immobilized Enzymes in Food Industry

Overviewing the Application of β-Galactosidase “Immobilized on Nanoparticles” in Dairy Industries

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