Immobilization of a cold-adaptive recombinant Penicillium cyclopium lipase on modified palygorskite for biodiesel preparation

Tan, Zhiyong; Zhou, Jia; Li, Xiangqian; Ren, Shiying; You, Qidong; Bilal, Muhammad

doi:10.1007/s13399-021-02006-7

Cited by 9 publications

(2 citation statements)

References 82 publications

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“…Enzyme immobilization is a useful technique developed in the 1950s; for example, Penicillium lipase was immobilized on modified palygorskite to prepare biodiesel, clay nanocomposites can improve the catalytic performance of lipase A from Candida antarctica in which the fixation of enzymes on nanomaterials is a sustainable and processable production method, , and lignocellulose residues are also a good immobilization carrier . Immobilized enzymes may have many advantages, e.g., easy separation, enhanced enzyme stability, elevated yield and quality of the product, and improved efficiency of the enzyme .…”

Section: Ai-aided Lipase Productionmentioning

confidence: 99%

Artificial Intelligence Aided Lipase Production and Engineering for Enzymatic Performance Improvement

Ge,

Chen,

Qian

et al. 2023

J. Agric. Food Chem.

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With the development of artificial intelligence (AI), tailoring methods for enzyme engineering have been widely expanded. Additional protocols based on optimized network models have been used to predict and optimize lipase production as well as properties, namely, catalytic activity, stability, and substrate specificity. Here, different network models and algorithms for the prediction and reforming of lipase, focusing on its modification methods and cases based on AI, are reviewed in terms of both their advantages and disadvantages. Different neural networks coupled with various algorithms are usually applied to predict the maximum yield of lipase by optimizing the external cultivations for lipase production, while one part is used to predict the molecule variations affecting the properties of lipase. However, few studies have directly utilized AI to engineer lipase by affecting the structure of the enzyme, and a set of research gaps needs to be explored. Additionally, future perspectives of AI application in enzymes, including lipase engineering, are deduced to help the redesign of enzymes and the reform of new functional biocatalysts. This review provides a new horizon for developing effective and innovative AI tools for lipase production and engineering and facilitating lipase applications in the food industry and biomass conversion.

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Section: Ai-aided Lipase Productionmentioning

confidence: 99%

Artificial Intelligence Aided Lipase Production and Engineering for Enzymatic Performance Improvement

Ge,

Chen,

Qian

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

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“…Pichia pastoris recombinant cold-adaptive lipase (rePcLip) from Penicillium cyclopium was immobilized on modified palygorskite to develop a robust biocatalytic system for biodiesel production from soybean oil. After reusing immobilized rePcLip for eight cycles, the residual activity and biodiesel yield were 71% and 65%, respectively [ 25 ]. Fan et al successfully used modified palygorskite as a carrier to immobilize plant esterase [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of EreB on Acid-Modified Palygorskite for Highly Efficient Degradation of Erythromycin

et al. 2022

IJERPH

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Erythromycin is one of the most commonly used macrolide antibiotics. However, its pollution of the ecosystem is a significant risk to human health worldwide. Currently, there are no effective and environmentally friendly methods to resolve this issue. Although erythromycin esterase B (EreB) specifically degrades erythromycin, its non-recyclability and fragility limit the large-scale application of this enzyme. In this work, palygorskite was selected as a carrier for enzyme immobilization. The enzyme was attached to palygorskite via a crosslinking reaction to construct an effective erythromycin-degradation material (i.e., EreB@modified palygorskite), which was characterized using FT-IR, SEM, XRD, and Brunauer–Emmett–Teller techniques. The results suggested the successful modification of the material and the loading of the enzyme. The immobilized enzyme had a higher stability over varying temperatures (25–65 °C) and pH values (6.5–10.0) than the free enzyme, and the maximum rate of reaction (Vmax) and the turnover number (kcat) of the enzyme increased to 0.01 mM min−1 and 169 min−1, respectively, according to the enzyme-kinetics measurements. The EreB@modified palygorskite maintained about 45% of its activity after 10 cycles, and degraded erythromycin in polluted water to 20 mg L−1 within 300 min. These results indicate that EreB could serve as an effective immobilizing carrier for erythromycin degradation at the industrial scale.

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Screening, identification, and characterization of lipase-producing halotolerant Bacillus altitudinis Ant19 from Antarctic soil

2023

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Immobilization of a cold-adaptive recombinant Penicillium cyclopium lipase on modified palygorskite for biodiesel preparation

Cited by 9 publications

References 82 publications

Artificial Intelligence Aided Lipase Production and Engineering for Enzymatic Performance Improvement

Artificial Intelligence Aided Lipase Production and Engineering for Enzymatic Performance Improvement

Immobilization of EreB on Acid-Modified Palygorskite for Highly Efficient Degradation of Erythromycin

Screening, identification, and characterization of lipase-producing halotolerant Bacillus altitudinis Ant19 from Antarctic soil

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