Immobilization of a-Amylase by SBA-15

Abstract

; AJC-15110 SBA (Santa Barbara Amorphous)-15 mesoporous material was prepared by hydrothermal synthesis and the sample was characterized by X-ray diffraction and scanning electron microscopy. The immobilization of α-amylase by SBA-15 was made by physical adsorption method and the optimal conditions of immobilization of α-amylase by SBA-15 were obtained as follows: pH = 5.4, α-amylase concentration:2 mg/mL, immobilization time:27 h, temperature:30 °C. At the same time, it is concluded that the maximum amount of αamylase immobilized by SBA-15 was 87.5 mg/g. By studying of chemical analysis, scanning electron microscopy, infrared spectroscopy, X-ray diffraction, low temperature N2 adsorption-desorption analysis characterization, the α-amylase was immobilized on the SBA-15 carrier was proved. Catalytic experimental results indicated that (SBA-15)-(α-amylase) composite material has the advantages of high pH value resistance and high temperature resistance compared with the free enzyme and has a higher potential applied value.