Immobilization and detection of DNA on microfluidic chips

Shamansky, Lisa M.; Davis, C.; Stuart, Joan K.; Kuhr, Werner G.

doi:10.1016/s0039-9140(01)00501-x

Cited by 51 publications

(41 citation statements)

References 16 publications

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“…The polymer films were electrogenerated on the quartz resonator. Then the modified electrode was washed with CH 3 CN for the poly[1 ± 3] and copoly[6,N-methylpyrrole] films or H 2 O for the poly [4,5,7] films before being immersed in an aqueous PBS solution (700 mL). Once resonance frequency reached a steady-state value, 10 mL of an avidin solution (2.5 mg mL À1 in PBS) was added to the buffer solution and the frequency was monitored with time.…”

Section: Electrochemical and Gravimetric Measurementsmentioning

confidence: 99%

“…The hydrophilic/hydrophobic effect of the polymer matrix on the avidin adsorption has also been investigated by QCM. The frequency variations recorded for the poly [1] and poly [4] films are 35 and 27 Hz respectively (Table 1). Taking into account the measurement uncertainly, it appears that the avidin adsorption is weakly reduced onto the hydrophilic film poly [4] compared to the hydrophobic film poly [1].…”

Section: Nonspecific Interaction Of Avidinmentioning

confidence: 99%

“…The frequency variations recorded for the poly [1] and poly [4] films are 35 and 27 Hz respectively (Table 1). Taking into account the measurement uncertainly, it appears that the avidin adsorption is weakly reduced onto the hydrophilic film poly [4] compared to the hydrophobic film poly [1]. Nevertheless this result is in agreement with those reported previously by Y. Chen et al [28] for protein adsorption on a polyaniline film modified by poly(ethyleneglycol).…”

Section: Nonspecific Interaction Of Avidinmentioning

confidence: 99%

“…To study this effect, gravimetric measurements using QCM have been performed on homopolymers poly [1] of various thickness. In addition we have compared the amount of avidin adsorbed on poly [1] and poly [4] films in order to investigate the influence of the hydrophobic/hydrophilic character of the polymer. Indeed, it is known avidin adsorbs strongly on the hydrophobic surface through hydrophobic interaction [20].…”

mentioning

confidence: 99%

“…In the second part of this article, the immobilization of avidine on a biotinylated polypyrrole film is studied by QCM on copolymers copoly [1,3] and copoly [4,5] electrosynthesized from an equimolar solution of monomers 1, 3 and 4, 5, respectively. The aim of this study is to evaluate the influence of the hydrophilic/hydrophobic nature of the polymer matrix on the amount of avidin immobilized by specific recognition.…”

mentioning

confidence: 99%

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Investigation by QCM of the Specific and Nonspecific Avidin Interaction onto a Biotinylated Polypyrrole Film

et al. 2004

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We have studied by gravimetric measurements using a quartz crystal microbalance (QCM), the adsorption of avidin onto a polypyrrole film. From QCM results, it appears that both the thickness and the redox state of polypyrrole strongly influence the avidin adsorption. This nonspecific interaction is larger for a thick film than for a thin one and, also for when the polymer is in its reduced state. We have also investigated by QCM the specific and nonspecific interaction of avidin onto biotinylated polypyrrole films having either a hydrophilic or hydrophobic character. It is shown that the amount of avidin immobilized by specific recognition is almost the same whatever the nature of the polymer matrix.

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Section: Electrochemical and Gravimetric Measurementsmentioning

confidence: 99%

Section: Nonspecific Interaction Of Avidinmentioning

confidence: 99%

Section: Nonspecific Interaction Of Avidinmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Investigation by QCM of the Specific and Nonspecific Avidin Interaction onto a Biotinylated Polypyrrole Film

et al. 2004

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Introduction

Seliger

2007

Microarrays

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Microarray technology has its roots in high-throughput parallel synthesis of biomacromolecules, combined with combinatorial science. In principle, the preparation of arrays can be performed either by in situ synthesis of biomacromolecules on solid substrates or by spotting of ex situ synthesized biomacromolecules onto the substrate surface. The application of microarrays includes spatial addressing with target (macro) molecules and screening for interactions between immobilized probe and target. The screening is simplified by the microarray format, which features a known structure of every immobilized library element. The area of nucleic acid arrays is best developed, because such arrays are allowed to follow the biosynthetic pathway from genes to proteins, and because nucleic acid hybridization is a most straightforward screening tool. Applications to genomics, transcriptomics, proteomics, and glycomics are currently in the foreground of interest; in this postgenomic phase they are allowed to gain new insights into the molecular basis of cellular processes and the development of disease.

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Substrate Patterning and Activation Strategies for DNA Chip Fabrication

Campo¹,

Bruce

2005

Topics in Current Chemistry

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Immobilization and detection of DNA on microfluidic chips

Cited by 51 publications

References 16 publications

Investigation by QCM of the Specific and Nonspecific Avidin Interaction onto a Biotinylated Polypyrrole Film

Investigation by QCM of the Specific and Nonspecific Avidin Interaction onto a Biotinylated Polypyrrole Film

Introduction

Substrate Patterning and Activation Strategies for DNA Chip Fabrication

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