Immobilization and characterization of horseradish peroxidase into chitosan and chitosan/PEG nanoparticles: A comparative study

Melo, Micael Nunes; Pereira, Fernanda Menezes; Rocha, Matheus; Ribeiro, Jesica Gonçalves; Diz, Fernando Mendonça; Monteiro, Wesley F.; Ligabue, Rosane Angélica; Severino, Patrícia; Fricks, Alini Tinoco

doi:10.1016/j.procbio.2020.08.007

Cited by 45 publications

(37 citation statements)

References 85 publications

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“…To verify the loading of the LO, and functionalization of anti-HER, the IR spectrum of free LO, HER, CS-LO NPs, CS-LO PEG NPs were compared, and the results are shown in Figure 2 a,b. The CS exhibited the characteristic vibration at 3351 cm −1 , 1648 cm −1 , and 1577 cm −1 assigned to the O-H stretching, C=O, amine I, and amine II respectively, while the 1419 cm −1 , 1375 cm −1 , 1314 cm −1 corresponding to O-H, and other characteristics peaks at 1060, 1026 assigned for C-N amine while peaks including 894 cm −1 , 555 cm −1 , 445 cm −1 accounted for C-H bending of CS [ 37 , 38 ]. The IR spectrum of the LO displayed primary protein absorption characteristic peaks at 3377 cm −1 accounting for N-H (primary amine), while 988 corresponded to C=C bending [ 39 ], Similarly, the protein absorption peaks for the lipase B and horseradish peroxidase are reported in the earlier work by using the IR spectrum [ 38 , 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

“…Besides, some of the peaks related to the LO changed due to the interaction of amino groups with polymer surfaces [ 40 ]. In addition, the peaks of 1240 cm −1 and 899 cm −1 in CS-LO NPs indicate the formation of P=O and P-O-P bonds related to the interaction of an amino group of CS and the phosphate group of TPP mediated CS NPs formation [ 38 , 42 ].…”

Section: Resultsmentioning

confidence: 99%

“…The PEG 6000 illustrated the characteristic peaks at 2880 cm −1 , 2740 cm −1 , 2694 cm −1 , corresponding to C-H, and 1466 cm −1 and 960 cm −1 accounted for by the ether bond [ 38 ]. The CS-LO PEG NPs exhibited the overlapping of the characteristic peaks, CS, LO and PEG, specifically the peaks such as 2882 cm −1 , 1575 cm −1 , 1466 cm −1 , 961 cm −1 that reappeared in the IR spectrum of CS-LO PEG NPs.…”

Section: Resultsmentioning

confidence: 99%

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Monoclonal Antibody Functionalized, and L-lysine α-Oxidase Loaded PEGylated-Chitosan Nanoparticle for HER2/Neu Targeted Breast Cancer Therapy

et al. 2022

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Herein, we designed a nanocarrier to deliver the LO specifically to HER2+ breast cancer (BC) cells, where functionalization of mAb (anti-HER2+) with PEGylated chitosan enabled it to target the HER2+ BC cells. Taking advantage of overexpression of HER2+ in cancer cells, our nanocarrier (CS-LO-PEG-HER NPs) exhibited promising potency and selectivity against HER2+ BC cells (BT474). The CS-LO-PEG-HER NPs demonstrated the cytotoxicity in BT474 cells by promoting reactive oxygen species, mitochondrial membrane potential loss, and nucleus damage. The biocompatibility of CS-LO-PEG-HER NPs was evidenced by the hemolysis assay and H & E staining of major organs. The CS-LO-PEG-HER NPs showed anticancer potency against the BT474-xenograft tumor-bearing mice, as evident by the reduction of tumor size and cell density. These results indicate that CS-LO-PEG-HER NPs are biocompatible with mice while inhibiting tumor growth through alter the oxidative stress. Overall, this work provides a promising approach for the delivery of LO for good therapeutic effect in combination with mAb.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Monoclonal Antibody Functionalized, and L-lysine α-Oxidase Loaded PEGylated-Chitosan Nanoparticle for HER2/Neu Targeted Breast Cancer Therapy

et al. 2022

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“…The authors specifically proposed producing recombinant HRP and utilizing it in antibody-directed enzyme prodrug therapy in combination with IAA (Figure 4). Chitosan and chitosan-PEG nanoparticles were demonstrated to immobilize HRP and thus influence its activity [86]. At a pH of 7, no significant differences between the activities of free HRP and HRP immobilized on chitosan (HRP/CS) and chitosan-PEG (HRP/CS-PEG) were observed.…”

Section: Peroxidasementioning

confidence: 98%

Advances on Delivery of Cytotoxic Enzymes as Anticancer Agents

Asrorov

Muhitdinov

et al. 2022

Molecules

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Cancer is one of the most serious human diseases, causing millions of deaths worldwide annually, and, therefore, it is one of the most investigated research disciplines. Developing efficient anticancer tools includes studying the effects of different natural enzymes of plant and microbial origin on tumor cells. The development of various smart delivery systems based on enzyme drugs has been conducted for more than two decades. Some of these delivery systems have been developed to the point that they have reached clinical stages, and a few have even found application in selected cancer treatments. Various biological, chemical, and physical approaches have been utilized to enhance their efficiencies by improving their delivery and targeting. In this paper, we review advanced delivery systems for enzyme drugs for use in cancer therapy. Their structure-based functions, mechanisms of action, fused forms with other peptides in terms of targeting and penetration, and other main results from in vivo and clinical studies of these advanced delivery systems are highlighted.

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“…[ 19 , 20 , 21 ]. Many approaches for tannase immobilization have been proposed, for example, the use of carboxyl-functionalized Fe 3 O 4 nanoparticles [ 22 ], chitin-alginate composites [ 23 ], and chitosan [ 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Chitosan Activated with Genipin: A Nontoxic Natural Carrier for Tannase Immobilization and Its Application in Enhancing Biological Activities of Tea Extract

et al. 2021

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In this work, a non-toxic chitosan-based carrier was constructed via genipin activation and applied for the immobilization of tannase. The immobilization carriers and immobilized tannase were characterized using Fourier transform infrared spectroscopy and thermogravimetric analysis. Activation conditions (genipin concentration, activation temperature, activation pH and activation time) and immobilizations conditions (enzyme amount, immobilization time, immobilization temperature, immobilization pH, and shaking speed) were optimized. The activity and activity recovery rate of the immobilized tannase prepared using optimal activation and immobilization conditions reached 29.2 U/g and 53.6%, respectively. The immobilized tannase exhibited better environmental adaptability and stability. The immobilized tannase retained 20.1% of the initial activity after 12 cycles and retained 81.12% of residual activity after 30 days storage. The catechins composition analysis of tea extract indicated that the concentration of non-ester-type catechins, EGC and EC, were increased by 1758% and 807% after enzymatic treatment. Biological activity studies of tea extract revealed that tea extract treated with the immobilized tannase possessed higher antioxidant activity, higher inhibitory effect on α-amylase, and lower inhibitory effect on α-glucosidase. Our results demonstrate that chitosan activated with genipin could be an effective non-toxic carrier for tannase immobilization and enhancing biological activities of tea extract.

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Immobilization and characterization of horseradish peroxidase into chitosan and chitosan/PEG nanoparticles: A comparative study

Cited by 45 publications

References 85 publications

Monoclonal Antibody Functionalized, and L-lysine α-Oxidase Loaded PEGylated-Chitosan Nanoparticle for HER2/Neu Targeted Breast Cancer Therapy

Monoclonal Antibody Functionalized, and L-lysine α-Oxidase Loaded PEGylated-Chitosan Nanoparticle for HER2/Neu Targeted Breast Cancer Therapy

Advances on Delivery of Cytotoxic Enzymes as Anticancer Agents

Chitosan Activated with Genipin: A Nontoxic Natural Carrier for Tannase Immobilization and Its Application in Enhancing Biological Activities of Tea Extract

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