Immobilised peptide displaying phages as affinity ligands

Noppe, Wim; Plieva, Fatima M.; Galaev, Igor Yu.; Vanhoorelbeke, Karen; Mattìasson, Bo; Deckmyn, Hans

doi:10.1016/j.chroma.2005.09.064

Cited by 46 publications

(33 citation statements)

References 25 publications

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“…In the first case, the cryopolymerization proceeded in the non-frozen microphase remaining after freezing of the reaction feed and resulted in the formation of a macroporous structure with highly interconnected pores. It should be noted that such structures have been successfully used as chromatographic monolithic sorbents for chromatography of particulate-containing fluids [18,26,33,60], or microbial cells [28,30,61,62], or as cell culture reactors [41].…”

Section: Control Of Porous Propertiesmentioning

confidence: 99%

Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate‐containing fluids and cell culture applications

Plieva

Galaev

Mattìasson³

2007

J of Separation Science

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180

134

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Macroporous gels (MGs) with a broad variety of morphologies are prepared using the cryotropic gelation technique, i. e. gelation at subzero temperatures. These highly elastic hydrophilic materials can be produced from practically any gel-forming system with a broad range of porosity extending from elastic and porous gels with pore sizes up to 1.0 microm to elastic and sponge-like gels with pore sizes up to 100 microm. The versatility of the cryogelation technique is demonstrated by use of different chemical reactions (hydrogen bond formation, chemical cross-linking of polymers, free radical polymerization) mainly in an aqueous medium. Appropriate control over solvent crystallization (formation of solvent crystals) and rate of chemical reaction during the cryogelation allows the reproducible preparation of cryogels with tailored properties. Different approaches, such as chemical modification of reactive groups, grafting of the pore surface with an appropriate polymer, or direct copolymerization with functional monomers are used for control of the surface chemistry of MGs. Typically, MGs with pore sizes up to 1.0 microm are produced in the shape of beads and MGs with pore size up to 100 microm are prepared as monoliths, discs, and sheets. The difference in porous structure of MGs defines the main applications of these porous materials. Elastic beaded MGs are mostly used as carriers for cell and enzyme immobilization or for capture of low-molecular weight targets from particulate-containing fluids in expanded-bed mode. However, the elastic and sponge-like MG monoliths with interconnected pores measuring hundreds of mum have been successfully used as monolithic columns for chromatography of particulate-containing fluids (crude cell homogenates, viruses, whole cells, wastewater effluents) and as three-dimensional scaffolds for mammalian cell culture applications.

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Section: Control Of Porous Propertiesmentioning

confidence: 99%

Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate‐containing fluids and cell culture applications

Plieva

Galaev

Mattìasson³

2007

J of Separation Science

Self Cite

180

134

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“…After brief exposition of the M13-His SPMs to a NaOH solution, the labeled anti-phage antibody was still able to recognize the target epitope on the modified phage-SPM. This is not surprising as the whole phage structure is naturally robust toward extreme pH and temperature conditions [21,22,29,31,37,38] . Fe 3+ leakage was not experimentally observed.…”

Section: Separation and Immunoblottingmentioning

confidence: 99%

“…The phage structure is extraordinarily robust, being resistant to heat, organic solvents, acidic conditions, alkali conditions and even 6 M urea [29,37] . Purified phage particle can also be stored at moderate temperature without jeopardizing its infectivity and/or binding activity [21,22,31,38] .…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the bacteriophage has been extensively used for molecular engineering [14,15] and other applications [16,17] . Phagebased materials have been developed for biomedical applications including biosensing [18,19] , affinity purification [20][21][22] , in vivo imaging [23][24][25] , drug/gene delivery, and antibiotic therapies [26,28] . The M13 bacteriophage is 6 nm in diameter and ~900 nm long virus [29] with a large surface area of 18,700 nm 2 [30] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Affinity Separation: M13 Bacteriophage‐Activated Superparamagnetic Beads for Affinity Separation (Small 15/2012)

Muzard¹,

Platt²,

G³

2012

Small

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“…The authors describe a low ionic strength buffer at pH 4 to elute fibrinogen from the column with high purity and almost no loss of activity. Importantly, peptides also exhibit exceptional selectivity, and have been successfully used to sequester and purify proteins from crude starting sources such as soy whey mixtures, whole blood and milk , Noppe et al 2006.…”

Section: Synthetic Peptidesmentioning

confidence: 99%

Advances in affinity ligand‐functionalized nanomaterials for biomagnetic separation

Fields

O'Mahony

et al. 2015

Biotech & Bioengineering

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The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the dependence on chromatography, while still adhering to regulatory concerns over product purity and safety. In this article, we present an up-to-date view of bioseparation, with emphasis on magnetic separation and its potential application in the field. Additionally, we discuss the economic and performance benefits of synthetic ligands, in the form of peptides and miniaturized antibody fragments, compared to full-length antibodies. We propose that adoption of synthetic affinity ligands coupled with magnetic adsorbents, will play an important role in enabling sustainable bioprocessing in the future.

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Immobilised peptide displaying phages as affinity ligands

Cited by 46 publications

References 25 publications

Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate‐containing fluids and cell culture applications

Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate‐containing fluids and cell culture applications

Affinity Separation: M13 Bacteriophage‐Activated Superparamagnetic Beads for Affinity Separation (Small 15/2012)

Advances in affinity ligand‐functionalized nanomaterials for biomagnetic separation

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