Immobilisation of Delta-like 1 ligand for the scalable and controlled manufacture of hematopoietic progenitor cells in a stirred bioreactor

Moore, Rebecca; Worrallo, Matthew J.; Mitchell, Peter D.; Harriman, J.; Glen, Katie E.; Thomas, Robert J.

doi:10.1186/s12896-017-0383-0

Cited by 4 publications

(2 citation statements)

References 45 publications

(43 reference statements)

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“…Usually, the immobilized surface is functionalized with either avidin or streptavidin, while biotin is coupled to the molecule of interest. In some instances, it is necessary to test GF immobilization efficiency depending on either the biotin-GF binding or the biotin-streptavidin binding occurring first (Moore et al, 2017). In most approaches, GF immobilization by biotin-streptavidin interactions involves the use of tetrameric streptavidin, which makes the biotinavidin/streptavidin interaction non-reversible.…”

Section: Biotin-streptavidin Interactionsmentioning

confidence: 99%

Immobilization of Growth Factors for Cell Therapy Manufacturing

Enriquez-Ochoa

Robles-Ovalle

Mayolo‐Deloisa

et al. 2020

Front. Bioeng. Biotechnol.

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Cell therapy products exhibit great therapeutic potential but come with a deterring price tag partly caused by their costly manufacturing processes. The development of strategies that lead to cost-effective cell production is key to expand the reach of cell therapies. Growth factors are critical culture media components required for the maintenance and differentiation of cells in culture and are widely employed in cell therapy manufacturing. However, they are expensive, and their common use in soluble form is often associated with decreased stability and bioactivity. Immobilization has emerged as a possible strategy to optimize growth factor use in cell culture. To date, several immobilization techniques have been reported for attaching growth factors onto a variety of biomaterials, but these have been focused on tissue engineering. This review briefly summarizes the current landscape of cell therapy manufacturing, before describing the types of chemistry that can be used to immobilize growth factors for cell culture. Emphasis is placed to identify strategies that could reduce growth factor usage and enhance bioactivity. Finally, we describe a case study for stem cell factor.

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Section: Biotin-streptavidin Interactionsmentioning

confidence: 99%

Immobilization of Growth Factors for Cell Therapy Manufacturing

Enriquez-Ochoa

Robles-Ovalle

Mayolo‐Deloisa

et al. 2020

Front. Bioeng. Biotechnol.

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“…[ 97 ] The combination of these systems could enable the scaled‐up feeder‐ and xeno‐free expansion of HSPC, together with their differentiation towards T lineage, for adoptive cell therapies. [ 98 ]…”

Section: Thymus Tissue Engineeringmentioning

confidence: 99%

Recapitulation of Thymic Function by Tissue Engineering Strategies

Silva

Reis

Martins

et al. 2021

Adv Healthcare Materials

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The thymus is responsible for the development and selection of T lymphocytes, which in turn also participate in the maturation of thymic epithelial cells. These events occur through the close interactions between hematopoietic stem cells and developing thymocytes with the thymic stromal cells within an intricate 3D network. The complex thymic microenvironment and function, and the current therapies to induce thymic regeneration or to overcome the lack of a functional thymus are herein reviewed. The recapitulation of the thymic function using tissue engineering strategies has been explored as a way to control the body's tolerance to external grafts and to generate ex vivo T cells for transplantation. In this review, the main advances in the thymus tissue engineering field are disclosed, including both scaffold-and cell-based strategies. In light of the current gaps and limitations of the developed systems, the design of novel biomaterials for this purpose with unique features is also discussed.

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The role of laboratory-scale bioreactors at the semi-continuous and continuous microbiological and biotechnological processes

Tikhomirova

Taraskevich

Ponomarenko

2018

Appl Microbiol Biotechnol

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Laboratory-scale bioreactors represent an important part of microbiological, biotechnological, and biochemical researches. The small volume bioreactors enable the biomass growth, extracellular metabolite production, solid-state fermentation, and biocatalytic processes in a controlled open system. Using the lab-scale bioreactors, the processes which were optimized in experiments can be scaled up to a pilot level. In turn, the manufacturing scale processes can be scaled down to study the heterogeneity of the system and its effect on the producer. This mini-review is focused on a short description of features of the most popular designs of continuous and semi-continuous bioreactors applied at the present time as well as their application area.

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Immobilisation of Delta-like 1 ligand for the scalable and controlled manufacture of hematopoietic progenitor cells in a stirred bioreactor

Cited by 4 publications

References 45 publications

Immobilization of Growth Factors for Cell Therapy Manufacturing

Immobilization of Growth Factors for Cell Therapy Manufacturing

Recapitulation of Thymic Function by Tissue Engineering Strategies

The role of laboratory-scale bioreactors at the semi-continuous and continuous microbiological and biotechnological processes

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