Immobilisation of cell‐binding peptides on poly‐ε‐caprolactone (PCL) films: A comparative XPS study of two chemical surface functionalisation methods

Stevens, Joanna S.; Luca, Alba C. de; Downes, S.; Terenghi, Giorgio; Schroeder, Sven L. M.

doi:10.1002/sia.5396

Cited by 17 publications

(15 citation statements)

References 17 publications

(31 reference statements)

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“…Figure b shows the Fe 2p spectrum corresponding to Fe 2p 3/2 (711.7 eV) and Fe 2p 1/2 (725.6 eV) peaks (Minati et al, ). The N 1s of RGD/Mal‐SPIONs spectrum is curve fitted into three different peaks (Figure c), and the binding energies at 401.9, 400.1 and 398.9 eV can be attributed to the CNH 3 + , CON, N–CO (or CN) groups of RGD, respectively (Bogdanowicz et al, ; Gopidas & Kamat, ; Oliveira, Martins, Mafra, & Gomes, ; Stevens, de Luca, Downes, Terenghi, & Schroeder, ). As shown in Figure d, the S 1s spectra of BSA/Mal‐SPIONs (Figure d), and the binding energies at about 167.6 and 163.1 eV can be ascribed to the SS and CSC groups of BSA/Mal‐SPIONs, respectively (Bogdanowicz et al, ; Liu et al, ; Mai et al, ; Shevchenko, Zaitsev, & Walcarius, ; Stevens et al, ).…”

Section: Resultsmentioning

confidence: 99%

Subcellular distributions of iron oxide nanoparticles in rat brains affected by different surface modifications

Wang

Zhang

et al. 2019

J Biomedical Materials Res

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The impact of the surface modification on the subcellular distribution of nanoparticles in the brain remains elusive. The nanoparticles prepared by conjugating polyethylene glycol and maleic anhydride‐coated superparamagnetic iron oxide nanoparticles (Mal‐SPIONs) with bovine serum albumin (BSA/Mal‐SPIONs) and with Arg–Gly–Asp peptide (RGD/Mal‐SPIONs) were injected into the rat substantia nigra. Observation of transmission electron microscopy (TEM) samples obtained 24 h after perfusion showed that abundant RGD/Mal‐SPIONs accumulated in the myelin sheath, dendrites, axon terminals and mitochondria, and on cell membranes in the brain tissue near the injection site. For rats injected with BSA/Mal‐SPIONs, a few nanoparticles accumulated in the myelin sheath, axon terminals, endoplasmic reticulum, mitochondria, Golgi, and lysosomes of neurons and glial cells while least SPIONs in rats injected with Mal‐SPIONs were found. TEM pictures showed some Mal‐SPIONs were expelled out of the brain. RGD/Mal‐SPIONs diffused extensively to the thalamus, frontal cortex, temporal lobe, olfactory bulb, and brain stem after injection. Only a few BSA/Mal‐SPIONs diffused to the afore‐mentioned brain areas. This work reveals different surface modifications on the iron oxide nanoparticles play crucial roles in their distribution and diffusion in the rat brains.

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Section: Resultsmentioning

confidence: 99%

Subcellular distributions of iron oxide nanoparticles in rat brains affected by different surface modifications

Wang

Zhang

et al. 2019

J Biomedical Materials Res

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“…Those differences might be resulted from the different C‐H/C‐C content on the surface. During the solification, the composite polymers intend to form a structure with minimized surface energy, therefore, more hydrophobic C‐H/C‐C was exposed on the surface .…”

Section: Resultsmentioning

confidence: 99%

Superhydrophobic PCL/PS composite nanofibrous membranes prepared through solution blow spinning with an airbrush for oil adsorption

Yin

et al. 2018

Polymer Engineering & Sci

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Superhydrophobic composite nanofibrous membranes(PP) were prepared from polycaprolactone (PCL)/polystyrene (PS) through solution blow spinning with an airbrush for oil adsorption. PP membranes were characterized by fourier transform infrared spectroscopy, scanning electron microscopy, water contact angle, X‐ray Photoelectron Spectroscopy, X‐ray diffractometer, and thermogravimetric analysis instrument. The effects of mass ratio of PCL and PS on the morphology, porosity, density, cyrstallinity, thermal stability, and oil adsorption ability were investigated. PP membranes had higher density than PCL and PS, while better hydrophobicity. The saturation adsorption capacity of PP to peanut oil, motor oil, and diesel oil is 16.89 g/g, 20.33 g/g, and 12.17 g/g, respectively. After 6 cycles of reutilization, the adsorption capacity to peanut oil, motor oil, and diesel oil remained at 6.16, 7.46, and 5.33 g/g, respectively, higher than that of pure PCL membrane.The water/oil adsorption selectivity of PP was about 7:100. PP had higher oil adsorption capacities and better water/oil adsorption selectivity than the commercial oil absorbent polypropylene. PP membrane could be prepared and used in situ to separate oil from oil/water mixture. POLYM. ENG. SCI., 59:E171–E181, 2019. © 2018 Society of Plastics Engineers

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“…Functionalization of the surface is generally done by the coupling of small molecules via covalent, ionic or hydrophobic interactions. [14,15] On the other hand, surface coating can be done by depositing the molecule of interest via methods such as plasma spraying, thermal spraying or electrolytic deposition. [16,17] For hydrophobic polyesters like PCL these strategies are of particularly interest for making their surface more cell adherent and biocompatible to induce favorable interactions with proteins and cells.…”

Section: Introductionmentioning

confidence: 99%

Long-term hydrolytic degradation study of polycaprolactone films and fibers grafted with poly(sodium styrene sulfonate): Mechanism study and cell response

et al. 2020

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Polycaprolactone (PCL) is a widely used biodegradable polyester for tissue engineering applications when long-term degradation is preferred. In this article, we focused on the analysis of the hydrolytic degradation of virgin and bioactive poly(sodium styrene sulfonate) (pNaSS) functionalized PCL surfaces under simulated physiological conditions (phosphate buffer saline at 25°C and 37°C) for up to 120 weeks with the aim of applying bioactive PCL for ligament tissue engineering. Techniques used to characterize the bulk and surface degradation indicated that PCL was hydrolyzed by a bulk degradation mode with an accelerated degradationthree times increased rate constant -for pNaSS grafted PCL at 37°C when compared to virgin PCL at 25°C.The observed degradation mechanism is due to the pNaSS grafting process (oxidation, radical polymerization) which accelerated the degradation until 48 weeks, when a steady state is reached. The PCL surface was altered by the pNaSS grafting, introducing hydrophilic sulfonate groups that increase the swelling and smoothing of the surface, which facilitated the degradation. After 48 weeks, pNaSS was largely removed from surface and the degradation of virgin and pNaSS grafted surfaces were similar. The cell response of primary fibroblast cells from sheep ligament were consistent with the surface analysis results: a better initial spreading of cells on pNaSS surfaces when compared to virgin surfaces and a tendency to become similar with degradation time.It is worthy to note that during the extended degradation process the surfaces were able to continue inducing better cell spreading plus preserve their cell phenotype as shown by collagen genes expressions.

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Immobilisation of cell‐binding peptides on poly‐ε‐caprolactone (PCL) films: A comparative XPS study of two chemical surface functionalisation methods

Cited by 17 publications

References 17 publications

Subcellular distributions of iron oxide nanoparticles in rat brains affected by different surface modifications

Subcellular distributions of iron oxide nanoparticles in rat brains affected by different surface modifications

Superhydrophobic PCL/PS composite nanofibrous membranes prepared through solution blow spinning with an airbrush for oil adsorption

Long-term hydrolytic degradation study of polycaprolactone films and fibers grafted with poly(sodium styrene sulfonate): Mechanism study and cell response

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