2019
DOI: 10.1016/j.heliyon.2019.e01546
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Immersion anaesthesia with ethanol in African giant land snails (Acathina fulica)

Abstract: Giant African land snails ( Achatina fulica ) are becoming increasingly popular pets and may be anaesthetised to allow diagnostics and surgical procedures. The objective of the present study was to evaluate the anaesthetic effects and anaesthetic-related complications of immersion in 5% ethanol in client-owned African pet land snails, anaesthetised to allow biopsies of the foot for screening of parasites. Variables such as minutes elapsing from immersion to anaesthetic induction and from… Show more

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Cited by 8 publications
(2 citation statements)
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“…Eighteen snails were chosen at random from each replicate after each time interval and anesthetized using 5% ethanol (d’Ovidio et al 2019 ). The snail tissues (hepatopancreas and head-foot) of the selected snails were quickly dissected, cleaned with 0.9% cold saline, weighed, and homogenized with 5 volumes of cold saline for 60s by a Polytron homogenizer (Tekmar tissumizer, Ohio, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Eighteen snails were chosen at random from each replicate after each time interval and anesthetized using 5% ethanol (d’Ovidio et al 2019 ). The snail tissues (hepatopancreas and head-foot) of the selected snails were quickly dissected, cleaned with 0.9% cold saline, weighed, and homogenized with 5 volumes of cold saline for 60s by a Polytron homogenizer (Tekmar tissumizer, Ohio, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Chemical agents such as isoflurane, carbon dioxide, tricaine and benzocaine have been shown to be effective anesthesia agents, but ethanol is more cost-effective and readily available [ [13] , [14] , [15] ]. Low concentration ethanol solutions (<10%) have previously been used to anesthetize some terrestrial and aquatic invertebrates but not Collembola [ 15 17 ]. The induction of chill comas to invertebrates is another simple and cost-effective method to temporarily immobilize invertebrates.…”
Section: Introductionmentioning
confidence: 99%