Immediate Inflammatory Response and Scar Formation in Wounded Vocal Folds

Lim, Xinhong; Tateya, Ichiro; Tateya, Tomoko; Muñoz-del-Rio, Alejandro; Bless, Diane M.

doi:10.1177/000348940611501212

Cited by 84 publications

(139 citation statements)

References 42 publications

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“…One application in the field of voice production is the characterization of viscoelastic properties of rat vocal fold tissue. Rats are commonly used animal models to study vocal fold biology and disease such as scarring and its treatments (Lim et al, 2006;Welham et al, 2008;Quinchia Johnson et al, 2010;Suehiro et al, 2010;Gugatschka et al, 2011). Characterization of the viscoelastic properties of rat tissue is difficult with other measurement methods such as tensile and rheology testing because of small sample size.…”

Section: B Perspectives For Future Workmentioning

confidence: 99%

Experimental methods for the characterization of the frequency-dependent viscoelastic properties of soft materials

Kazemirad

Heris

Mongeau

2013

The Journal of the Acoustical Society of America

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A characterization method based on Rayleigh wave propagation was developed for the quantification of the frequency-dependent viscoelastic properties of soft materials at high frequencies; i.e., up to 4 kHz. Planar harmonic surface waves were produced on the surface of silicone rubber samples. The phase and amplitude of the propagating waves were measured at different locations along the propagation direction, which allowed the calculation of the complex Rayleigh wavenumbers at each excitation frequency using a transfer function method. An inverse wave propagation problem was then solved to obtain the complex shear/elastic moduli from the measured wavenumbers. In a separate, related investigation, dynamic indentation tests using atomic force microscopy (AFM) were performed at frequencies up to 300 Hz. No systematic verification study is available for the AFM-based method, which can be used when the dimensions of the test samples are too small for other existing testing methods. The results obtained from the Rayleigh wave propagation and AFM-based indentation methods were compared with those from a well-established method, which involves the generation of standing longitudinal compression waves in rod-shaped test specimens. The results were cross validated and qualitatively confirmed theoretical expectations presented in the literature for the frequency-dependence of polymers.

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Section: B Perspectives For Future Workmentioning

confidence: 99%

Experimental methods for the characterization of the frequency-dependent viscoelastic properties of soft materials

Kazemirad

Heris

Mongeau

2013

The Journal of the Acoustical Society of America

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“…10,11 TGF-β gene expression increases as early as 8 hours after vocal fold injury and is maximal at 72 hours (the end point of the study). 11 In addition, hepatocyte growth factor has been shown to be an effective inhibitor of TGF-β-induced vocal fold fibrosis, both in vivo and in vitro. [12][13][14][15][16] A clinical report suggested that treatment with tranilast, a TGF-β inhibitor, reduced glottic fibrosis associated with intubation injury.…”

Section: Introductionmentioning

confidence: 99%

Effects of Transforming Growth Factor-β1 on Human Vocal Fold Fibroblasts

Branski¹,

Barbieri²,

Weksler³

et al. 2009

Ann Otol Rhinol Laryngol

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Insight into the underlying pathophysiology of vocal fold fibrosis is likely to yield improved therapeutic strategies to mitigate vocal fold scarring. Our data suggest that TGF-beta signaling may be both paracrine and autocrine in this vocal fold fibroblast cell line, and we therefore propose that TGF-beta may be a reasonable target for therapies to prevent and/or treat vocal fold fibrosis, given its putative role in both acute and chronic vocal fold injury, as well as its effects on vocal fold fibroblasts.

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“…Frequently, however, excessive scar formation closes this pressure-reducing valve, thereby forcing the intraocular pressure to rise (5). Moreover, excessive scarring of the vocal folds may severely alter their ability to vibrate, thereby causing a number of voice disorders (6).…”

mentioning

confidence: 99%

Collagen Fibril Formation

Chung

Steplewski

Chung

et al. 2008

Journal of Biological Chemistry

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We present a concept for reducing formation of fibrotic deposits by inhibiting self-assembly of collagen molecules into fibrils, a main component of fibrotic lesions. Employing monoclonal antibodies that bind to the telopeptide region of a collagen molecule, we found that blocking telopeptide-mediated collagen/collagen interactions reduces the amount of collagen fibrils accumulated in vitro and in keloid-like organotypic constructs. We conclude that inhibiting extracellular steps of the fibrotic process provides a novel approach to limit fibrosis in a number of tissues and organs.Collagen I is the most abundant structural protein of connective tissues such as skin, bone, and tendon. This protein is first synthesized as a precursor molecule, procollagen, that is characterized by the presence of a rod-like central triple-helical domain flanked by short linear telopeptides and globular N-terminal and C-terminal propeptides (1). Single procollagen molecules are the building blocks for the biologically and mechanically relevant collagen fibrils. Formation of collagen fibrils is initiated by enzymatic cleavage of the N-terminal and the C-terminal propeptides. The N-terminal propeptides are cleaved by a group of enzymes that includes a disintegrin and metalloprotease with thrombospondin motifs (ADAMTS)-2, -3, and -14, whereas the C-terminal propeptides are cleaved by the metalloprotease bone morphogenetic protein 1 (BMP-1) 2 and by the other members of a closely related family of mammalian tolloid-like metalloproteases (2-4). Such a removal of procollagen propeptides exposes telopeptides, which by engaging in site-specific intermolecular interactions drive collagen self-assembly.In native tissues a precise balance between the processes of biosynthesis and degradation maintains the physiological homeostasis of tissue collagens. At the same time, accelerated biosynthesis is required for proper wound healing, whereas excessive accumulation of collagen is the hallmark of a number of localized fibrotic diseases, such as keloids and hypertrophic scars, and systemic fibrosis, such as systemic scleroderma.Localized fibrotic reactions are quite common and frequently develop as a consequence of surgical procedures. For instance, after surgery of the abdomen, the formation of excessive scar tissue around abdominal organs, such as the intestines, can interfere with the functionality of such organs and may cause severe pain and even death. Another situation where excessive scar formation presents a major complication is in the eye after glaucoma surgery performed to create a pressuremaintenance valve. Frequently, however, excessive scar formation closes this pressure-reducing valve, thereby forcing the intraocular pressure to rise (5). Moreover, excessive scarring of the vocal folds may severely alter their ability to vibrate, thereby causing a number of voice disorders (6).At present, several biological processes critical for development of fibrotic lesions are considered potential targets for inhibitors of fibrosis. These inhibit...

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Immediate Inflammatory Response and Scar Formation in Wounded Vocal Folds

Abstract: The results show a clear profile of vocal fold inflammation with corresponding changes in extracellular matrix production.

Cited by 84 publications

References 42 publications

Experimental methods for the characterization of the frequency-dependent viscoelastic properties of soft materials

Experimental methods for the characterization of the frequency-dependent viscoelastic properties of soft materials

Effects of Transforming Growth Factor-β1 on Human Vocal Fold Fibroblasts

Collagen Fibril Formation

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