Immediate hypersensitivity to bakery, brewery and wine products in yeast‐sensitive atopic dermatitis patients

Abstract

Ultrafiltered (> 1000 Da) samples of beer, aged red wine, young white wine, sparkling wine and extracts of fresh wheat bread and dried rye bread were analysed by skin-prick test (SPT), radioallergosorbent test (RAST) inhibition, sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting to find out if they contain Saccharomyces cerevisiae (S. cerevisiae, baker's yeast) allergens. Serum pool consisting of S. cerevisiae positive sera was used in the assays. The results were compared with freeze-dried reference S. cerevisiae and cereal antigens. The beer, bread, red wine and sparkling wine extracts elicited immediate reactions. However, no evident correlation with suspected symptoms was observed. White wine extract caused reactions in four out of six atopic dermatitis (AD) patients with symptoms, and in five out of seven symptom-free AD patients and in two of the 24 controls. In SDS-PAGE, protein bands were found in wheat and rye bread extracts and beer. In IgE immunoblotting, however, no staining was seen with the S. cerevisiae positive sera suggesting that they were of cereal origin. In white wine and champagne extracts a non-specific staining was seen in the region 20 kDa representing, e.g. lectin-like activity. No baker's yeast antigen could be detected in brewery and bakery products with IgE-immunoblotting even in the excessively concentrated extracts. The IgE mediated allergy to baker's yeast alone should thus not lead to denial of bakery, brewery and wine products.

Dairy and nondairy beverage consumption for childhood atopic eczema: what health advice to give?

“…In general, avoidance of cow milk is recommended for young children with AE who are sensitized to cow milk . There is no good evidence that restriction of cow milk is either beneficial or detrimental in older AE children who are not sensitized to cow milk.…”

“…There is no good evidence that AE is aggravated by alcoholic beverages; however, alcoholic beverages are generally not recommended for young people. For older teenagers, avoidance or social consumption in moderation is again recommended …”

“…In general, avoidance of cow milk is recommended for young children with AE who are sensitized to cow milk. 4,[10][11][12][13][14][15][16][17] There is no good evidence that restriction of cow milk is either beneficial or detrimental in older AE children who are not sensitized to cow milk. The GABRIELA study reported a protective effect of farm milk consumption on childhood asthma and atopy, possibly attributable to the whey protein fraction of milk.…”

“…For older teenagers, avoidance or social consumption in moderation is again recommended. 13,14 Ideally, the exact amount of beverages consumed should be recorded. The volume consumed may be more important than the frequency consumed.…”

Enzyme immunoassays for specific IgG and IgE antibodies to Pichia pastoris components in normal humans

“…The reaction is dependent on the specific triggering of IgE-sensitized mast cells by antigen resulting in the release of pharmacological mediators of inflammation (24). Many researchers have reported about allergens of yeasts and specific IgE antibody to these allergens cross-reacted among the allergens of the different fungi (25)(26)(27)(28)(29). The antigen-specific IgE assay is now widely used as a replacement for the classical skin testing procedure used in allergy diagnosis.…”