Immediate-early gene region of human cytomegalovirus trans-activates the promoter of human immunodeficiency virus.

Davis, M. R.; Kenney, Shannon C.; Kamine, James; Pagano, Joseph S.; Huang, Eng‐Shang

doi:10.1073/pnas.84.23.8642

Cited by 292 publications

(151 citation statements)

References 17 publications

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“…As COS cells contain the large T antigen of simian virus (SV40), possible interactions between these two regulatory proteins cannot be ruled out in this system. Similar results were reported by Davis et al (1987), who found that cotransfection of an IE 1 expression plasmid together with a chloramphenicol acetyltransferase (CAT) hybrid gene, driven by the HCMV 1El regulatory region, down-regulates gene expression by a factor of eight in HeLa cells. However they could also report a stimulatory effect of HCMV IE gene products on the long terminal repeat of human immunodeficiency virus type 1, which was fused to the CAT gene.…”

Section: Functional Aspects Of Le Gene Productssupporting

confidence: 74%

Human Cytomegalovirus: Recent Aspects from Molecular Biology

Mach

Stamminger

Jahn

1989

Journal of General Virology

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Section: Functional Aspects Of Le Gene Productssupporting

confidence: 74%

Human Cytomegalovirus: Recent Aspects from Molecular Biology

Mach

Stamminger

Jahn

1989

Journal of General Virology

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“…Consistent with previous reports (Rando et al, 1987;Elfassi et al, 1987), we also find that HCMV can enhance HIV-1 LTR-directed transcription. The HCMV IE gene region encodes several different proteins (Stinski et al, 1983;Stenberg et al, 1984) and recent evidence has implicated the IE 2 gene in a number of trans-activating and autoregulating events (Hermiston et al, 1987;Pizzorno et al, 1988), including activation of HIV-I LTR-directed transcription (Davis et al, 1987;Rando et al, unpublished results). The isolation of HIV from peripheral blood leukocytes usually requires an exogenous signal associated with T-cell activation (Zagury et al, 1986) and the enhancer region of the HIV-LTR contains a recognition sequence for the transcriptional factor NF-kB, which is associated with T cell activation (Nabel & Baltimore, 1987).…”

Section: Discussionmentioning

confidence: 99%

“…Ho and others immediate early genes of HCMV) can increase HIV long terminal repeat (LTR)-directed transcription (Davis et al, 1987;Elfassi et al, 1987;Rando et al, 1987;Skolnik et al, 1988). In the present study, we have further analysed the molecular interactions of HCMV and HIV-1.…”

Section: Introductionmentioning

confidence: 99%

Reciprocal Enhancement of Gene Expression and Viral Replication Between Human Cytomegalovirus and Human immunodeficiency Virus Type 1

Harouse

Rando

et al. 1990

Journal of General Virology

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Biological interactions between human cytomegalovirus (HCMV) and the human immunodeficiency virus type 1 (HIV-1) were analysed in transfection and infection experiments, carried out in a human osteogenic sarcoma cell line (HOS) and in the same cell line chronically infected with HCMV (E155). When HOS and E155 cells were transfected with recombinant plasmids containing the HIV long terminal repeat (LTR) linked to the bacterial chloramphenicol acetyltransferase (CAT) gene, LTR-directed CAT expression was 20 times higher in E155 cells than in HOS cells. HOS cells co-infected with HCMV and HIV-1 showed enhanced production of the HIV-1 p24 antigen. In reciprocal experiments, an increase in HCMV immediate early gene expression was observed when HCMVinfected HOS cells and E155 cells were either transfected with a recombinant plasmid containing the HIV transactivator gene (pTAT), or when infected with HIV-1. DNA hybridization analysis of E155 and HCMV-infected HOS cells revealed higher levels of HCMV DNA in cells transfected with pTAT than in ceils transfected with other non-specific recombinant plasmids. E155 cells transfected with pTAT also produced higher titres of infectious HCMV than control cultures of E155 cells transfected with other recombinant plasmids, including pMTAT carrying a mutant tat gene. The functional reciprocity in vitro between HCMV and HIV is discussed with respect to its possible implications for the clinical development of AIDS.

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“…This 72-kDa phosphoprotein is abundantly expressed immediately after infection (Plachter et al, 1996). In transient transfection assays, IE1 has been shown to be involved in the autoregulation of its own promoter and also in the transactivation of viral and cellular promoters, alone or in concert with other viral or cellular proteins (Stenberg and Stinski, 1985;Davis et al, 1987;Tevethia et al, 1987;Cherrington and Mocarski, 1989;Sambucetti et al, 1989;Stenberg et al, 1989;Iwamoto et al, 1990;Hagemeier et al, 1992;Monick et al, 1992;Michelson et al, 1994;Margolis et al, 1995;Yurochko et al, 1995;Kim et al, 1999;Murayama et al, 2000). It was also reported that the IE1 gene product could cooperate with another IE gene product, IE2, and adenoviral E1A to transform primary baby rat kidney (BRK) cells (Shen et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of GFAP, TSP‐1, and p53 in human glioblastoma cell line, U373MG, by IE1 protein from human cytomegalovirus

Lee

Jeon

Kim

et al. 2005

Glia

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Human cytomegalovirus (HCMV) is a member of the b-herpesvirus family, which has tropism for glial cells. It was recently reported that HCMV might play important roles in the pathogenesis of malignant glioma. In this study, we investigated the effects of the HCMV IE1 protein on the gene expression profile in the human glioblastoma cell line, U373MG by employing cDNA microarray technology. Using DNA chips containing approximately 1,000 human cDNAs, RNA samples from U373MG cells stably expressing IE1 were compared with those from the control cells lacking IE1 cDNA. Fluorescence intensities of 13 genes were significantly decreased in IE1-expressing cells, while one gene was found to be upregulated. Among these 14 genes, we chose to work further on glial fibrillary acidic protein (GFAP), thrombospondin-1 (TSP-1), and p53, because of their previously known involvement in tumorigenesis. The mRNA levels of all these genes were found to be decreased in IE1-expressing glioblastoma cells by real-time quantitative reverse transcriptionpolymerase chain reaction (RT-PCR) as well as Northern blot analysis. The decreased expression of these genes was also observed at protein levels as measured by immunocytochemistry or fluorescence-activated cell sorting (FACS) analysis. Our data strongly suggested that HCMV IE1 could modulate the expression of cellular genes that might play important roles in the pathogenesis of glial tumors. '

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Immediate-early gene region of human cytomegalovirus trans-activates the promoter of human immunodeficiency virus.

Abstract: Almost all homosexual patients with acquired immunodeficiency syndrome are also actively infected with human cytomegalovirus (HCMV). We have hypothesized that an interaction between HCMV and human immunodeficiency virus

Cited by 292 publications

References 17 publications

Human Cytomegalovirus: Recent Aspects from Molecular Biology

Human Cytomegalovirus: Recent Aspects from Molecular Biology

Reciprocal Enhancement of Gene Expression and Viral Replication Between Human Cytomegalovirus and Human immunodeficiency Virus Type 1

Downregulation of GFAP, TSP‐1, and p53 in human glioblastoma cell line, U373MG, by IE1 protein from human cytomegalovirus

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