Immediate-early gene expression in structures outside the basal ganglia is associated to l-DOPA-induced dyskinesia

Selective Inactivation of Striatal FosB/ΔFosB-Expressing Neurons Alleviates L-DOPA–Induced Dyskinesia

“…We The FosB-lacZ lentivirus was injected in vivo to evaluate the role of FosB-expressing neurons on AIMs in 6-hydroxydopaminelesioned rats (6,8,18,39), the rodent analog of LID. After the establishment of stable AIMs with a therapeutic dose of 6 mg/kg L-dopa (6,8,(16)(17)(18), a single intrastriatal administration of Daun02 significantly decreased AIMs compared with control rats during 2 days (RM ANOVA; group  day: F 6,60 = 2.64; p Ͻ .05; Bonferroni: p Ͻ .05 for all; Figure 3A).…”

“…Experimental parkinsonism was achieved with a unilateral injection of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle as previously described (6,(16)(17)(18). Surgeries were conducted under isoflurane anesthesia.…”

“…On the last day, baseline AIMs were scored as previously described (6,8,(16)(17)(18) using a validated rating scale assessing three items (axial, forelimb, orolingual dyskinesia) rated from 0 (absent) to 4 (continuous, severe, uninterrupted by sensory distraction) (21) to obtain a composite score (maximum 12 points). Ratings lasted 1 minute per rat at 30, 60, 90, and 120 minutes post L-dopa.…”

Nanoparticles restore lysosomal acidification defects: Implications for Parkinson and other lysosomal-related diseases

“…For stereological counting of dopaminergic neurons and immunofluorescence, mice were terminated and brains were collected, immediately immersed in cold 4% paraformaldehyde for 24 h, then cryoprotected with 20% sucrose (Fluka, 84100) and finally frozen by immersion in isopentane at ¡50 C. 48 Brains were cut at 50-mm thick sections and SN was collected. For colocalization studies of PLGA-aNP with lysosomes in vivo, TH (tyrosine hydroxylase; Abcam, ab6211) and LAMP2 (Abcam, ab25339) immunohistochemistry was performed as previously described.…”

“…Unbiased stereological counting of THpositive neurons was performed using Exploranova Mercator (Explora Nova, La Rochelle, France) as previously described. 48,49 Ten sections per animal were used and optical dissectors were distributed using a systemic sampling scheme. Dissectors (50 mm £ 40 mm) were separated from each other by 150 mm (x) and 120 mm (y).…”