Immediate-Early Baculovirus Genes Transactivate the p143 Gene Promoter of Autographa californica Nuclear Polyhedrosis Virus

Lu, Albert; Carstens, Eric B.

doi:10.1006/viro.1993.1422

Cited by 96 publications

(79 citation statements)

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“…IE1 was initially characterized using plasmid transfection assays as a transcriptional activator for baculovirus early gene expression (Guarino & Summers, 1986, 1987Kovacs et al, 1991;Guarino & Smith, 1992;Nissen & Friesen, 1989;Pullen & Friesen, 1995;Blissard & Rohrmann, 1991;Theilmann & Stewart, 1991;Lu & Carstens, 1993;Ribeiro et al, 1994). In similar assays, IE1 has also been shown to be essential for the replication of plasmids carrying putative origins of baculovirus DNA replication (Kool et al, 1994;Ahrens & Rohrmann, 1995;Lu & Miller, 1995).…”

Section: Introductionmentioning

confidence: 99%

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Kawasaki

Matsumoto

Nagamine

2004

Journal of General Virology

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IE1, a principal transcriptional activator of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), is an essential factor for viral DNA replication. During viral infection, IE1 accumulates in discrete subnuclear structures where viral DNA replication occurs. To analyse the dynamic properties of IE1, we monitored green fluorescent protein-tagged IE1 (IE1-GFP) in BmNPV-infected B. mori cells by live-cell microscopy. Time-lapse imaging showed that IE1-associated structures gradually expanded and occasionally fused with one another, while photobleaching experiments revealed that IE1-GFP was relatively immobile inside the IE1-associated structures. To investigate the spatial relationships between IE1 and viral structural proteins in infected cells, three GFP-tagged viral components were expressed together with DsRed-tagged IE1. Two structural proteins that constitute the occlusion-derived virus (ODV), P91-GFP and GFP-ODV-E25, localized to the periphery of the IE1-associated structures. While local accumulations of these proteins were often in contact with the IE1-associated structures, they did not extend beyond the boundaries of the structures. In contrast, the major capsid protein VP39-GFP predominantly accumulated within the IE1-associated structures. These data indicated, in conjunction with the finding of a high DNA content in the structures, that IE1 localizes to the virogenic stroma and therefore support the prediction previously proposed that the virogenic stroma is a site for viral DNA replication as well as for the assembly of nucleocapsids.

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Section: Introductionmentioning

confidence: 99%

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Kawasaki

Matsumoto

Nagamine

2004

Journal of General Virology

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“…As demonstrated by transient expression studies, the activity of early promoters depends mostly on the presence of viral gene products in addition to host factors. The best known viral transactivator is IE1, which is able to activate several early, late and very late promoters , 1988Nissen & Friesen, 1989 ;Blissard & Rohrmann, 1991 ;Lu & Carstens, 1993 ;Passarelli & Miller, 1993). The AcMNPV genome has eight homologous regions (Ayres et al, 1994) containing multiple EcoRI sites which form the centre of imperfect palindromic sequences (Cochran & Faulkner, 1983).…”

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confidence: 99%

Baculovirus transactivator IE1 is functional in mammalian cells.

Murges

Kremer

Knebel-Mörsdorf

1997

Journal of General Virology

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“…A previous study by Lu and Carstens (1993) revealed that a greater transactivation ability of the helicase promoter was observed with PE-38 than with IE-1 in AcMNPV. But in our experiment, pe-38 didn't increase the activation of the helicase promoter from the BmNPV.…”

Section: Transactivation Effect Of the Early Gene On The Helicase Promentioning

confidence: 85%

Construction and Application of a Baculovirus Genomic Library

Chen

Lin

Yao

et al. 2009

Zeitschrift Für Naturforschung C

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A random genomic library of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) was constructed and viral factors were identifi ed by screening the regulator(s) for helicase gene expression. DNAs of 238 library plasmids were used to co-transfect with the reporter plasmid, pHp510-luc, in which the luciferase (luc) gene was driven by the baculovirus helicase promoter. Results showed that eight plasmids of the library strengthened the luciferase activity more than 1000-fold. Sequence analyses revealed that all of the eight plasmids contained an intact ie-1 coding region. To confi rm the reliability of the screening library, pHp510-luc was co-transfected with the cloned early gene which revealed that the BmNPV IE-1 was the only early factor that could stimulate the helicase promoter. The function analyses suggested that genome-wide screening factors through the library are powerful means to investigate the transcriptional regulation of dsDNA viruses.

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Immediate-Early Baculovirus Genes Transactivate the p143 Gene Promoter of Autographa californica Nuclear Polyhedrosis Virus

Cited by 96 publications

References 0 publications

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Baculovirus transactivator IE1 is functional in mammalian cells.

Construction and Application of a Baculovirus Genomic Library

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