Immediate and long-term transcriptional response of hind muscle tissue to transient variation of incubation temperature in broilers

Naraballobh, Watcharapong; Trakooljul, Nares; Muráni, Eduard; Brunner, Ronald M.; Krischek, Carsten; Janisch, S.; Wicke, Michael; Ponsuksili, Siriluck; Wimmers, Klaus

doi:10.1186/s12864-016-2671-9

Cited by 7 publications

(5 citation statements)

References 53 publications

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“…At the same time, these studies are indicative for the tissue-specific plasticity of expression patterns induced during different developmental stages (e.g. 6 , 7 ) and under varying environmental conditions 8 . The mitochondrial efficiency of energy production has been identified as one of the major molecular mechanisms driving FE in muscle tissue of meat-type chickens 9 .…”

Section: Introductionmentioning

confidence: 68%

Transcriptional shifts account for divergent resource allocation in feed efficient broiler chickens

Reyer

Metzler‐Zebeli

Trakooljul

et al. 2018

Sci Rep

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Considerable variation in feed efficiency (FE) has been observed in indigenous and selected meat-type chicken populations. Although this variation could be partially linked to extrinsic factors like diet, housing environment and microbiota, it further illustrates the existence of strong molecular mechanisms enabling the differential allocation of resources for various physiological processes. To further deepen the molecular basis of individual allocation capacity in male and female broilers, an RNA-seq experiment was conducted which based on a phenotyped chicken population divergent in FE. Transcriptional differences linked to FE were pronounced in intestinal and muscular tissue sites of male animals. Specifically, signalling pathways of farnesoid X receptor (FXR) and retinoid X receptor (RXR) might contribute to mediate individual FE. The transcriptional profiles suggested ACSBG2 (muscular lipid utilisation), ASBT (intestinal bile salt transport), CLEC2B (natural killer cell activation), HMGCS2 (jejunal, duodenal and muscular ketogenesis), and SCARB1 (jejunal lipid uptake) as potential mediators driving FE. Results indicate that improvements in FE exploit shifts in resource allocation which might occur at the expense of general immune responsiveness in high efficient male chickens. Consequently, to further improve FE traits and to explore causative molecular patterns, effects originating from sex-dimorphism in chickens need to be taken into consideration.

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Section: Introductionmentioning

confidence: 68%

Transcriptional shifts account for divergent resource allocation in feed efficient broiler chickens

Reyer

Metzler‐Zebeli

Trakooljul

et al. 2018

Sci Rep

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“…Moreover, we have previously shown consistency of microarray expression data with real time qPCR data using hind muscle tissue of the same animals. In fact, we obtained significant correlation coefficients ranging between 0.71 and 0.84 [ 16 ]. Annotated genes with different transcript abundances are termed “differentially expressed genes (DEGs)”; higher (lower) abundance in treated group vs. control groups is termed up-regulated (down-regulated).…”

Section: Methodsmentioning

confidence: 99%

Transient Shifts of Incubation Temperature Reveal Immediate and Long-Term Transcriptional Response in Chicken Breast Muscle Underpinning Resilience and Phenotypic Plasticity

et al. 2016

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Variations in egg incubation temperatures can have acute or long-term effects on gene transcription in avian species. Altered gene expression may, in turn, affect muscle traits in poultry and indirectly influence commercial production. To determine how changes in eggshell temperature affect gene expression, incubation temperatures were varied [36.8°C (low), 37.8°C (control), 38.8°C (high)] at specific time periods reflecting two stages of myogenesis [embryonic days (ED) 7–10 and 10–13]. Gene expression was compared between interventions and matching controls by microarrays in broiler breast muscle at ED10 or ED13 and post-hatch at day 35. Early (ED7-10) high incubation temperature (H10ΔC) resulted in 1370 differentially expressed genes (DEGs) in embryos. Ingenuity pathway analysis revealed temporary activation of cell maintenance, organismal development, and survival ability genes, but these effects were not maintained in adults. Late high incubation temperature (ED10-13) (H13ΔC) had slightly negative impacts on development of cellular components in embryos, but a cumulative effect was observed in adults, in which tissue development and nutrition metabolism were affected. Early low incubation temperature (L10ΔC) produced 368 DEGs, most of which were down-regulated and involved in differentiation and formation of muscle cells. In adults, this treatment down-regulated pathways of transcriptional processes, but up-regulated cell proliferation. Late low temperature incubation (L13ΔC) produced 795 DEGs in embryos, and activated organismal survival and post-transcriptional regulation pathways. In adults this treatment activated cellular and organ development, nutrition and small molecule activity, and survival rate, but deactivated size of body and muscle cells. Thermal interventions during incubation initiate immediate and delayed transcriptional responses that are specific for timing and direction of treatment. Interestingly, the transcriptional response to transiently decreased incubation temperature, which did not affect the phenotypes, prompts compensatory effects reflecting resilience. In contrast, higher incubation temperature triggers gene expression and has long-term effects on the phenotype. These mechanisms of considerable phenotypic plasticity contribute to the biodiversity and broaden the basis for managing poultry populations.

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“…Significant adverse effects on meat yield and muscle development have been observed with continuous or long-term (more than 6 h) cyclic high temperatures (>38°C) during incubation ( Hulet et al, 2007 ; Naraballobh et al, 2016a , b ; Clark et al, 2017 ). High incubation temperatures increase oxygen consumption of embryos during the late endothermic phase of incubation that occurs in the 3 to 4 days before hatch ( Janke et al, 2002 ; Mortola and Labbè, 2005 ; Molenaar et al, 2010 , 2013 ).…”

Section: Muscle Development In Avian Embryosmentioning

confidence: 99%

“…In several studies, it has been observed that variations on temperature during incubation affect myoblast proliferation and skeletal muscle hypertrophy in chickens ( Collin et al, 2007 ; Piestun et al, 2009 ; Janisch et al, 2015 ; Naraballobh et al, 2016a , b ), turkeys ( Maltby et al, 2004 ; Krischek et al, 2013a ), and ducks ( Hepp et al, 2006 ; Hopkins et al, 2011 ; Liu et al, 2015 ). Most of the studies have evaluated thermal modifications for the short term during different periods of incubation to stimulate muscle growth.…”

Section: Evidence Of Incubation Effects On Muscle Development and Myomentioning

confidence: 99%

The Role of Incubation Conditions in the Onset of Avian Myopathies

Oviedo-Rondón

Velleman

Wineland³

2020

Front. Physiol.

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White striping, wooden breast, and spaghetti muscle have become common myopathies in broilers worldwide. Several research reports have indicated that the origin of these lesions is metabolic disorders. These failures in normal metabolism can start very early in life, and suboptimal incubation conditions may trigger some of the key alterations on muscle metabolism. Incubation conditions affect the development of muscle and can be associated with the onset of myopathies. A series of experiments conducted with broilers, turkeys, and ducks are discussed to overview primary information showing the main changes in breast muscle histomorphology, metabolism, and physiology caused by suboptimal incubation conditions. These modifications may be associated with current myopathies. Those effects of incubation on myopathy occurrence and severity have also been confirmed at slaughter age. The impact of egg storage, temperature profiles, oxygen concentrations, and time of hatch have been evaluated. The effects have been observed in diverse species, genetic lines, and both genders. Histological and muscle evaluations have detected that myopathies could be induced by extended hypoxia and high temperatures, and those effects depend on the genetic line. Thus, these modifications in muscle metabolic responses may make hatchlings more susceptible to develop myopathies during grow out due to thermal stress, high-density diets, and fast growth rates.

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Immediate and long-term transcriptional response of hind muscle tissue to transient variation of incubation temperature in broilers

Cited by 7 publications

References 53 publications

Transcriptional shifts account for divergent resource allocation in feed efficient broiler chickens

Transcriptional shifts account for divergent resource allocation in feed efficient broiler chickens

Transient Shifts of Incubation Temperature Reveal Immediate and Long-Term Transcriptional Response in Chicken Breast Muscle Underpinning Resilience and Phenotypic Plasticity

The Role of Incubation Conditions in the Onset of Avian Myopathies

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