Immediate and late analysis of dental pulp stem cells viability after indirect exposition to alternative in-office bleaching strategies

Soares, Diana Gabriela; Basso, Fernanda Gonçalves; Hebling, Josimeri; Costa, Carlos Alberto de Souza

doi:10.1007/s00784-014-1321-3

Cited by 36 publications

(44 citation statements)

References 28 publications

(82 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In future studies, the best application time should be determined, as well as intervals between sessions to achieve better performance of the compound with minor adverse effects. 27,28 Subjective outcomes measured by the variation of SGU units are shown to be inconsistent with the objective results. The subjective determination of tooth colour is challenged by high variability.…”

Section: Discussionmentioning

confidence: 92%

“…10 Soares et al recently reported on low-concentration (17.5%) and short-duration applications and found significantly reduced cellular damage under in vitro conditions. [12][13][14] There is interest in RCTs to assess compounds with lower concentration that would comply with standards such as those of the European Community. The objective of this work is to show the effectiveness of a bleaching gel (6%) catalyzed by titanium dioxide nanoparticles and activated by hybrid light.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effectiveness of 6% hydrogen peroxide concentration for tooth bleaching—A double-blind, randomized clinical trial

Martín

Vildósola

Bersezio

et al. 2015

Journal of Dentistry

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Effectiveness of 6% hydrogen peroxide concentration for tooth bleaching—A double-blind, randomized clinical trial

Martín

Vildósola

Bersezio

et al. 2015

Journal of Dentistry

View full text Add to dashboard Cite

“…Thus, Jun‐D is present in pulp tissue during the repair process involving reactionary dentine formation, but its presence during dentine formation following severe loss of odontoblasts caused by intense H 2 O 2 ‐induced oxidative stress (Soares et al . , , Cintra et al . ) has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

In vivo analysis of the presence of heme oxygenase‐1, transcription factor Jun‐D and CD90+/CD73+/CD105+/CD45‐ cells in the pulp of bleached teeth

Benetti¹,

Briso

Lopes

et al. 2019

Int Endodontic J

View full text Add to dashboard Cite

Aim To investigate hydrogen peroxide (H2O2)‐induced responsiveness in pulp cells using heme oxygenase‐1 (HO‐1) immunolabelling, Jun‐D immunolabelling to study the effects of H2O2 on odontoblastic differentiation and CD90+/CD73+/CD105+/CD45‐ cell counting for in vivo identification of mesenchymal stem cells in the pulp. Methodology The maxillary molars of 50 rats were treated with a bleaching gel (35% H2O2, 1 × 30 min) or placebo gel (control groups). At 2, 3, 7, 15 and 30 days after the treatment (n = 10), inflammation in pulp tissue was analysed by haematoxylin–eosin staining, HO‐1‐ and Jun‐D‐immunolabelled cells were counted in each third of the pulp chamber, and the number of CD90+/CD73+/CD105+/CD45‐ cells was quantified by immunofluorescence. The results were assessed using the Paired t‐test or Wilcoxon signed‐rank test (P < 0.05). Results Significant H2O2‐induced inflammation was noted at 2 and 3 days (P < 0.05), with tertiary dentine formation occurring from 7 days. The bleached specimens had greater HO‐1 immunolabelling in the middle and cervical thirds of the coronal pulp at 2 and 3 days, in all thirds at 7 days, and in the occlusal third at 15 days (P < 0.05), and significant nuclear Jun‐D immunolabelling in the cervical third at 2 and 3 days and in the occlusal and middle thirds at 7 days (P < 0.05). Bleached and control groups had low numbers of CD90+/CD73+/CD105+/CD45‐ cells in the pulp at all periods (P > 0.05). Conclusions Pulp cells responded to oxidative stress by expressing HO‐1 during the post‐bleaching inflammation phase until the beginning of the repair phase. Jun‐D expression occurred during the reduction of inflammation and the beginning of tertiary dentine production. The presence of oxidative stress did not influence the number of CD90+/CD73+/CD105+/CD45‐ cells identified in vivo in the dental pulp.

show abstract

“…As oxygen diffuses through enamel and dentin, it may affect the pulp 11,12,18,21,28 and result in tooth sensibility, an adverse effect of toxic components 2,3,12,15,27 . The low molecular weight of hydrogen peroxide promotes tooth bleaching, but also the release of inflammatory mediators into the pulp, which may damage pulp cells 3,12,26,27 .…”

Section: Introductionmentioning

confidence: 99%

“…The low molecular weight of hydrogen peroxide promotes tooth bleaching, but also the release of inflammatory mediators into the pulp, which may damage pulp cells 3,12,26,27 . The rate of hydrogen peroxide penetration differs between bleaching agents and depends on their concentration and time of application as well as on the presence or absence of restorations 3,17,21 .…”

Section: Introductionmentioning

confidence: 99%

Inflammatory response of human dental pulp to at-home and in-office tooth bleaching

et al. 2016

View full text Add to dashboard Cite

Tooth bleaching is a technique of choice to obtain a harmonious smile, but bleaching agents may damage the dental pulp.Objective:This study evaluated the inflammatory responses of human dental pulp after the use of two bleaching techniques.Material and Methods:Pulp samples were collected from human third molars extracted for orthodontic reasons and divided into three groups: control - no tooth bleaching (CG) (n=7); at-home bleaching with 15% carbamide peroxide (AH) (n = 10), and in-office bleaching with 38% hydrogen peroxide (IO) (n=12). Pulps were removed and stained with hematoxylin-eosin for microscopic analysis of inflammation intensity, collagen degradation, and pulp tissue organization. Immunohistochemistry was used to detect mast cells (tryptase+), blood vessels (CD31+), and macrophages (CD68+). Chi-square, Kruskal-Wallis, and Mann Whitney tests were used for statistical analysis. The level of significance was set at p<.05.Results:The inflammation intensity and the number of macrophages were significantly greater in IO than in AH and CG (p<0.05). The results of CD31+ (blood vessels per mm2) were similar in CG (61.39±20.03), AH (52.29±27.62), and IO (57.43±8.69) groups (p>0.05). No mast cells were found in the pulp samples analyzed.Conclusion:In-office bleaching with 38% hydrogen peroxide resulted in more intense inflammation, higher macrophages migration, and greater pulp damage then at-home bleaching with 15% carbamide peroxide, however, these bleaching techniques did not induce migration of mast cells and increased the number of blood vessels.

show abstract

Immediate and late analysis of dental pulp stem cells viability after indirect exposition to alternative in-office bleaching strategies

Cited by 36 publications

References 28 publications

Effectiveness of 6% hydrogen peroxide concentration for tooth bleaching—A double-blind, randomized clinical trial

Effectiveness of 6% hydrogen peroxide concentration for tooth bleaching—A double-blind, randomized clinical trial

In vivo analysis of the presence of heme oxygenase‐1, transcription factor Jun‐D and CD90+/CD73+/CD105+/CD45‐ cells in the pulp of bleached teeth

Inflammatory response of human dental pulp to at-home and in-office tooth bleaching

Contact Info

Product

Resources

About