Immediate and delayed (after cooling) effects of centrifugation on equine sperm

Len, Jose; Jenkins, Jill A.; Eilts, B.E.; Paccamonti, Dale L.; Lyle, Sara K.; Hosgood, Giselle

doi:10.1016/j.theriogenology.2009.09.003

Cited by 21 publications

(17 citation statements)

References 21 publications

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“…Literature reporting the analysis of equine semen describes a wide variety of methodologies both in CASA motility settings [16–19] and in the use of different chambers in combination with CASA systems. The chambers most frequently used are a 20 µm deep Leja b chamber [17,19–21], 20 µm deep Cell‐vu c chamber [22–24] and 10 µm deep Makler a chamber [25,26]. The different chambers often accommodate different volumes of semen and there is no standardisation in reporting other variables.…”

Section: Introductionmentioning

confidence: 99%

Influence of counting chamber type on CASA outcomes of equine semen analysis

Hoogewijs

Vliegher

Govaere

et al. 2011

Equine Veterinary Journal

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Section: Introductionmentioning

confidence: 99%

Influence of counting chamber type on CASA outcomes of equine semen analysis

Hoogewijs

Vliegher

Govaere

et al. 2011

Equine Veterinary Journal

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“…Los diferentes diseños de cámara afectan tanto la distribución final de los espermatozoides dentro de la cámara como las características de movilidad y cinética. Esto se ha demostrado en diversas especies como el verraco (Christensen et al, 2005), el bovino (Prathalingam et al, 2006;Contri et al, 2010;Lenz et al, 2011;Gloria et al, 2013), el caprino (Del-Gallego et al, 2017), el humano (Tomlinson et al, 2001;Peng et al, 2015), conejo (Massányi et al, 2008), ovino (Palacín et al, 2013) y el equino (Len et al, 2010;Spizziri et al, 2010;Hoogewijs et al, 2012). En especies como la caprina, la movilidad progresiva y las velocidades de los espermatozoides fueron mayores con cámaras de carga capilar a alturas de 20 μm respecto de 10 μm (Del-Gallego et al, 2017).…”

Section: Discussionunclassified

Evaluación de cámaras de recuento sobre parámetros espermáticos de verracos analizados con un sistema CASA-Mot

Valverde

Madrigal‐Valverde

2019

Agron. Mesoam.

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Introducción. La comprensión de la variabilidad en los valores de la cinética de los espermatozoides, por medio de diferentes profundidades de cámaras, demuestra la importancia de crear un estándar para los métodos de control de calidad en la industria de la inseminación artificial (IA). Objetivo. El objetivo del presente trabajo fue evaluar los parámetros de cinética de los espermatozoides con base en diferentes profundidades de cámara de visualización mediante un sistema comercial de análisis de esperma asistidos por computadora, CASA-Mot. Materiales y Métodos. Se utilizaron veinte dosis seminales de diez verracos pietrain. El periodo experimental fue de febrero a julio del año 2017. Se utilizó el sistema integrado de análisis de semen (ISAS®v1) con una frecuencia de captura de 50 Hz. Se utilizaron cámaras de recuento ISAS®D4C16 e ISAS®D4C20 con una altura de 16 y 20 μm, respectivamente y precalentadas a 37 °C. Resultados. Se encontraron valores superiores (p<0,05) para todos los parámetros cinéticos cuando la altura de la cámara de recuento fue de 20 μm. El efecto de zona dentro de la cámara de recuento se mantuvo constante entre las dos alturas, y las variaciones observadas en los parámetros cinéticos se debieron a un efecto aleatorio del verraco. Al analizar el efecto de zona dentro de la cámara de recuento, los primeros tres campos de análisis mostraron mayor velocidad curvilínea y rectilínea (p<0,05) que los siguientes campos, lo que se atribuye a la presencia de movimiento pasivo (drifting). Conclusión. La mayor amplitud y capacidad de volumen dentro de la cámara de recuento (20 μm versus 16 μm), podría favorecer el desplazamiento sin restricciones de las células, lo que explicaría el incremento en los valores cinéticos conforme aumentó la altura de la cámara. Deben continuarse los estudios sobre las condiciones técnicas del análisis seminal para estandarizar los métodos de valoración con los sistemas CASA.

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“…This could have been due to several factors such as the reduced volume of semen and the centrifugation time used in the present study. Unlike the protocols for swine and stallions, it uses the cushioned centrifugation for large volumes of ejaculate and a longer duration of centrifugation (BLISS et al, 2012;LEN et al, 2010;MATÁS et al, 2007). Therefore, this study reports for the first time, the use of CF for the selection of cryopreserved bovine semen.…”

Section: Discussionmentioning

confidence: 99%

“…Other methods of sperm selection, such as the swimup method reduce the use of centrifugation, but this technique is still necessary to concentrate the sample. Studies report that high centrifugal forces often promote higher sperm recovery (WAITE et al, 2008;LEN et al, 2010;GLORIA et al, 2016) Some studies suggested that a reduction in the force and duration of centrifugation could reduce sperm damage and improve the sperm selection protocol using Percoll gradient (GUIMARÃES et al, 2014;KIM et al, 2013). However, these studies evaluated only the first centrifugation for sperm selection disregarding the second centrifugation for sperm concentration.…”

Section: Introductionmentioning

confidence: 99%

Use of a colloid to optimize centrifugation in the selection of bovine sperm for IVF

Gonçalves

Leivas

Míssio

et al. 2018

SCA

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This study aimed to evaluate the effect of the force and duration of centrifugation and the impact of cushioned centrifugation on sperm selection by Percoll gradient, on sperm quality and development kinetics of in vitro produced bovine embryos. Two experiments were performed. In Experiment I, a pool of semen was selected by Percoll gradients and the pellet was divided into four groups and distributed in a 2 × 2 factorial, with two forces (2200 × g or 9000 × g) and two durations (1 min or 3 min) of centrifugation. In Experiment II, semen was divided into two groups and selected by Percoll gradient with Cushion Fluid (CF) or without CF (Control) in the second centrifugation. The morphofunctionality, biochemical characteristics and fertilizing capacity of the selected sperms were evaluated. In addition, the development of the resulting bovine embryos was monitored for 48 h post-insemination. Duncan and Chi-square tests (P < 0.05) were used to compare the means. In Experiment I, there was a significant increase in sperm vigor (P < 0.05) after sperm selection in all treatments. The force and duration of centrifugation did not have any effect on sperm motility, vigor, and recovery rate among the different treatments (P > 0.05). In Experiment II, the recovery rate and reactive oxygen species (ROS) production in semen were similar among treatments (P > 0.05) although a higher ROS production was observed in the CF fertilization medium. Total fertilization rate was superior in the CF group (65.4 ± 5.3%) compared to that in Control (39.6 ± 4.9%). However, the normal fertilization and cleavage rate did not differ between the Control (94 ± 6.3% and 58.3 ± 8.3%) and CF (89 ± 7.1% and 75.0 ± 7.3%) groups. The reduction in the force and duration of centrifugation did not decrease the sperm recovery during selection by the Percoll gradient and the use of CF in the second centrifugation did not affect the normal fertilization and development of bovine IVF embryos up to 48 h. Key words: Bovine embryo. Cushion centrifugation. In vitro production. Percoll gradients. Sperm recovery. ResumoEsse estudo objetivou avaliar o efeito da força e duração da centrifugação, e o impacto da centrifugação amortecida na seleção espermática por gradientes de Percoll, na qualidade espermática e cinética do desenvolvimento de embriões bovinos produzidos in vitro. Dois experimentos foram realizados. No experimento I, um pool de sêmen foi selecionado por gradientes de Percoll e o pellet dividido em quatro grupos e distribuído em um fatorial 2 x 2, com duas forças (2200 e 9000 X g) e dois tempos (1 e 3 min) de centrifugação. No Experimento II, o sêmen foi dividido em dois grupos e selecionado com (CF) ou sem CushionFluid (Controle) na segunda centrifugação. A morfofuncionalidade, características bioquímicas e capacidade fecundante dos espermatozoides selecionados foram avaliadas. Além disso, o desenvolvimento dos embriões bovinos resultantes foi monitorado por 48 horas pós-inseminação. Os testes Duncan e Qui-quadrado (P<0,05) foram usados para ...

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Immediate and delayed (after cooling) effects of centrifugation on equine sperm

Cited by 21 publications

References 21 publications

Influence of counting chamber type on CASA outcomes of equine semen analysis

Influence of counting chamber type on CASA outcomes of equine semen analysis

Evaluación de cámaras de recuento sobre parámetros espermáticos de verracos analizados con un sistema CASA-Mot

Use of a colloid to optimize centrifugation in the selection of bovine sperm for IVF

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