Immature reticulocyte fraction as a criterion for marrow engraftment. Evaluation of a semi-automated reticulocyte counting method

Grotto, Helena Zerlotti Wolf; Vigoritto, A C; Noronha, José F. A.; Lima, G. A. L. M.

doi:10.1046/j.1365-2257.1999.00239.x

Cited by 14 publications

(14 citation statements)

References 3 publications

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“…We have applied the same criterion previously, using semi-automated equipment and similar results were obtained. A rising IRF was the first haematological sign of recovery in all patients studied (n ¼ 10), preceding the rise in neutrophils and platelets (Grotto et al, 1999).…”

Section: Discussionmentioning

confidence: 79%

“…Dalal et al (1996) used quantification of younger reticulocytes, expressed as the reticulocyte maturity index (RMI), and defined engraftment as the first day of a 3-day trend of RMI ‡2.0. A rising IRF was the first haematological sign of recovery in all patients studied (n ¼ 10), preceding the rise in neutrophils and platelets (Grotto et al, 1999). Torres et al (2001), using the Pentra 120 Retic, defined erythroid engraftment as the first post-transplantation day when the MFI and IRF values reached 10 and 10%, respectively, with HFR higher than 3% for at least three consecutive days.…”

Section: Discussionmentioning

confidence: 92%

“…In a previous study, MFI proved to be more stable and more reproducible than other reticulocyte maturation parameters (Grotto et al, 1999). Therefore, we used that parameter as an index of reticulocyte immaturity.…”

Section: Haematological Recoverymentioning

confidence: 99%

“…Reticulocyte counts performed by automated flow cytometric methods is a good indicator of erythropoietic activity and its evaluation has been proposed as an early predictor of bone marrow regeneration after transplantation (Davis et al, 1989;Davies et al, 1992;Greinix et al, 1994;Gerritsen et al, 1996;Grotto et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Immature reticulocytes as an early predictor of engraftment in autologous and allogeneic bone marrow transplantation

Noronha

Souza

Vigorito

et al. 2003

Clinical &Amp; Laboratory Haematology

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The purpose of this study was to evaluate reticulocyte parameters by means of flow cytometric reticulocyte counting in a group of patients who had undergone autologous and allogeneic bone marrow transplantation (BMT). The pattern of reticulocyte response and the predictive value of absolute neutrophil count (ANC), platelet count, number of CD34+ cell infused and graft source for reticulocyte response were studied. We compared absolute reticulocyte count (RetAbs), mean fluorescence index (MFI) and mean reticulocyte volume/mean corpuscular volume (MRV/MCV) ratio with conventional criteria (ANC and platelet count) in 22 allogeneic and 20 autologous BMT recipients. An abrupt increase in MRV/MCV ratio or a rise in MFI value were the earliest signs of erythropoietic recovery following allogeneic transplantation (63.6 and 22.8% of cases, respectively). In 13.6% of the cases, both parameters were observed simultaneously. All but three autologous transplant recipients showed changes in reticulocyte parameters earlier than ANC recovery. Granulocyte recovery and peripheral blood progenitor cells (PBPC) graft were predictive variables for RetAbs response in allogeneic transplant recipients. In the autologous group, predictive variables for RetAbs response were a high number of CD34+ infused cells and platelet recovery. An increase in the immature reticulocyte population is the earliest sign of haematopoietic recovery following BMT.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 92%

Section: Haematological Recoverymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Immature reticulocytes as an early predictor of engraftment in autologous and allogeneic bone marrow transplantation

Noronha

Souza

Vigorito

et al. 2003

Clinical &Amp; Laboratory Haematology

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“…The immature reticulocyte fraction (IRF) is a recently described fraction of reticulocytes that is determined by the relative amount of RNA present in the reticulocyte (Chang & Kass, 1997). This index has been used as an early marker of engraftment following autologous (George et al, 2000;Noronha et al, 2003) and allogeneic transplantation (Grotto et al, 1999;Torres et al, 2001;Noronha et al, 2003). The IRF has also previously been used to monitor stem cell collection, with a rise in IRF thought to predict for stem cell collection (Remacha et al, 1996;Mougi, Shinmyozu & Osame, 1997).…”

Section: Introductionmentioning

confidence: 99%

The immature reticulocyte fraction: a negative predictor of the harvesting of CD34 cells for autologous peripheral blood stem cell transplantation

Dunlop

Cohen

Harvey

et al. 2006

Clinical &Amp; Laboratory Haematology

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The optimal time for the harvesting of peripheral blood stem cells following chemotherapy and growth factors for autologous transplantation is based on the CD34 cell count. In this study, 51 patients having 59 stem cell mobilizations were assessed for the timing of the harvest by a CD34 cell count and an immature reticulocyte fraction (IRF). Results from 272 preharvest tests showed that when the CD34 cells were not harvestable, defined as a CD34 cell count of < 15 cells/microl, the IRF was always < or = 0.2. A low IRF resulted in a negative predictive value of 1 for the harvesting of stem cells. The IRF is therefore a valuable negative predictor of the timing of autologous stem cell harvesting.

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Performing a Blood Count

2006

Blood Cells: A Practical Guide

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Immature reticulocyte fraction as a criterion for marrow engraftment. Evaluation of a semi-automated reticulocyte counting method

Cited by 14 publications

References 3 publications

Immature reticulocytes as an early predictor of engraftment in autologous and allogeneic bone marrow transplantation

Immature reticulocytes as an early predictor of engraftment in autologous and allogeneic bone marrow transplantation

The immature reticulocyte fraction: a negative predictor of the harvesting of CD34 cells for autologous peripheral blood stem cell transplantation

Performing a Blood Count

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