Immature oocyte quality and maturational competence of porcine cumulus-oocyte complexes subpopulations

Álvarez, Gabriel Martín; Dalvit, Gabriel Carlos; Achi, María Verónica; Miguez, Marcelo Sergio; Cetica, Pablo Daniel

doi:10.32604/biocell.2009.33.167

Cited by 31 publications

(3 citation statements)

References 47 publications

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“…The research of Alvarez et al (17) showed that cumulus cells have an important role in oocyte maturation to metaphase-II stage, therefore, oocytes with compact cumulus mass are used for in vitro maturation protocols. For this reason, oocytes with incomplete cumulus mass or <3 layers of cumulus cells, are usually discarded from further evaluation.…”

Section: Discussionmentioning

confidence: 99%

Brilliant Cresyl Blue Staining Enhances the Morphological Quality Evaluation of Porcine Oocytes-a Preliminary Study

Dovenska

Atanasov

Benc

et al. 2023

Macedonian Veterinary Review

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Brilliant cresyl blue (BCB) staining is a method used for selection of developmentally competent porcine oocytes, in addition to the standard morphological classification. The aim of the current study was to assess the relationship between BCB staining in porcine oocytes with high and low morphological characteristics and its maturation rate. In the first part of the study, cumulus-oocytes complexes (COCs) (n=434) were aspirated from 60 ovaries. According to the morphological classification and BCB staining, they were divided in 4 groups: COC+/BCB+, COC−/BCB+, COC+/BCB−, and COC−/BCB−. In the second experiment, COCs (n=445) were categorized into 3 groups: control, BCB+, and BCB−. Significant differences in the maturation rate were observed between COC+/BCB+ and COC−/BCB− (66% vs. 23%), and between COC+/BCB+ and COC−/BCB+ (66% vs. 38%) (p<0.0001). Significant differences were also registered between COC−/BCB+ and COC−/BCB-(38% vs. 23%) and between COC+/BCB− and COC−/BCB− (53% vs. 23%) (p<0.01). Results from the second experiment showed that only BCB− oocytes had a significantly lower maturation rate (28%) compared to the control (63%) and BCB+ oocytes (59%) (p<0.001). These findings showed that COCs with high morphological characteristics had higher developmental ability compared to COCs with low morphology. The BCB-staining in high-quality oocytes did not have a significant impact on their maturation ability compared to a control group, but it might be useful for selecting developmentally competent oocytes with low morphology characteristics.

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Section: Discussionmentioning

confidence: 99%

Brilliant Cresyl Blue Staining Enhances the Morphological Quality Evaluation of Porcine Oocytes-a Preliminary Study

Dovenska

Atanasov

Benc

et al. 2023

Macedonian Veterinary Review

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“…After incubation, they were washed twice in a phosphate buffer saline (PBS) + polyvinyl alcohol (PVA) solution and loaded on a glass slide for their observation under an epifluorescent microscope. Live oocytes were distinguished based on their green fluorescence ( Alvarez et al , 2009 ).…”

Section: Methodsmentioning

confidence: 99%

Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming

Morado,

Aparicio,

Pinchetti

et al. 2023

Open Vet J

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Background: As the porcine oocyte is the most sensitive to low temperature damage, it has been difficult to cryopreserve compared to those from other domestic animals. However, at present, vitrification is used as a method for the cryopreservation of both oocytes and embryos in this species. Aim: Our aim was to analyze alterations in metabolic parameters in vitrified-warmed in vitro matured porcine oocytes at different post-warming recuperation times. Additionally, metaphase II plate recovery time analysis, in vitro fertilization and intracytoplasmic sperm injection were carried out to evaluate oocyte recovery capacity. Methods: Oocytes were vitrified-warmed and then incubated for 0, 3 or 21h post-warming to assess biochemical parameters. Results: Oocyte viability and morphology were not affected by vitrification-warming. Cytosolic oxidative status, active mitochondria and reactive oxygen species levels presented changes at the different time points in control and vitrified-warmed oocytes (P<0.05) as well as differences between both groups (P<0.05). NAD(P)H levels remained constant throughout different recuperation times, but were significantly lower in vitrified-warmed oocytes (P<0.05). Metaphase II plate recovery occurred mostly between 3 and 4h post-warming, but the percentage of metaphase II was reduced by vitrification-warming. Sperm head decondensation and pronuclear formation capacities were not modified. Conclusion: In conclusion, vitrification-warming generates biochemical alterations in porcine oocytes that would be, in part, responsible for affecting their performance. So, although the technique is a valid alternative for porcine oocyte cryopreservation, the protocols should be adapted to minimize those alterations.

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“…antioxidants [8,9] and cumulus features [10] can enhance the quality of porcine oocytes in IVM. Specifically, supplementation of antioxidant to IVM medium improved embryo quality, reduced early apoptosis levels, increased GSH content and reduced ROS accumulation, promoting embryonic development [11].…”

Section: Introductionmentioning

confidence: 99%

Rapamycin Treatment During Prolonged In Vitro Maturation Enhances the Developmental Competence of Immature Porcine Oocytes

Lee,

Yoon

et al. 2023

J Anim Sci Technol

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Porcine oocytes undergo in vitro maturation (IVM) for 42-44 h. During this period, most oocytes proceed to metaphase and then to pro-metaphase if the nucleus has sufficiently matured. Forty-four hours is sufficient for oocyte nuclear maturation but not for full maturation of the oocyte cytoplasm. This study investigated the influences of extension of the IVM duration with rapamycin treatment on molecular maturation factors. The phospho-p44/42 mitogen-activated protein kinase (MAPK) level was enhanced in comparison with the total p44/42 MAPK level after 52 h of IVM. Oocytes were treated with and without 10 μM rapamycin (10 R and 0 R, respectively) and examined after 52 h of IVM, whereas control oocytes were examined after 44 h of IVM. Phospho-p44/42 MAPK activity was upregulated the 10 R and 0 R oocytes than in control oocytes. The expression levels of maternal genes were highest in 10 R oocytes and were higher in 0 R oocytes than in control oocytes. Reactive oxygen species (ROS) activity was dramatically increased in 0 R oocytes but was similar in 10 R and control oocytes. The 10 R group exhibited an increased embryo development rate, a higher total cell number per blastocyst, and decreased DNA fragmentation. The mRNA level of development-related (POU5F1 and NANOG) mRNA, oocyte-apoptotic (BCL2L1) genes were highest in 10 R blastocysts. These results suggest that prolonged IVM duration with rapamycin treatment represses ROS production and increases expression of molecular maturation factors. Therefore, this is a good strategy to enhance the developmental capacity in porcine oocytes.

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Immature oocyte quality and maturational competence of porcine cumulus-oocyte complexes subpopulations

Cited by 31 publications

References 47 publications

Brilliant Cresyl Blue Staining Enhances the Morphological Quality Evaluation of Porcine Oocytes-a Preliminary Study

Brilliant Cresyl Blue Staining Enhances the Morphological Quality Evaluation of Porcine Oocytes-a Preliminary Study

Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming

Rapamycin Treatment During Prolonged In Vitro Maturation Enhances the Developmental Competence of Immature Porcine Oocytes

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