Imitation of drug metabolism in cell co-culture microcapsule model using a microfluidic chip platform coupled to mass spectrometry

Zheng, Y.; Wu, Zengnan; Lin, Jin‐Ming; Li, Gang

doi:10.1016/j.cclet.2019.07.036

Cited by 42 publications

(13 citation statements)

References 20 publications

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“…The water samples were acidified to pH 2.0 with H 2 SO 4 (2 M) and passed through extraction cartridges at a speed of 5 mL/min (Oasis hydrophilic lipophilic balance, 500 mg, 6 mL; Waters, U.S.A.). 42 Before extraction, the cartridges were activated with 10 mL of methanol and 10 mL of ultrapure water. The cartridge was washed with ultrapure water, completely dried under nitrogen flow, and eluted with 5 mL of methanol, 2 mL of acetone, and 2 mL of dichloromethane.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Toxicity of Ozonated Wastewater to HepG2 Cells: Taking Full Account of Nonvolatile, Volatile, and Inorganic Byproducts

Yang

et al. 2021

Environ. Sci. Technol.

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Wastewater ozonation forms various toxic byproducts, such as aldehydes, bromate, and organic bromine. However, there is currently no clear understanding of the overall toxicity changes in ozonated wastewater because pretreatment with solid phase extraction cannot retain inorganic bromate and volatile aldehydes, yet contributions of known ozonation byproducts to toxicity are unknown. Moreover, compared with bromate, organic bromine did not receive widespread attention. This study evaluated the toxicity of ozonated wastewater by taking aldehydes, bromate, and organic bromine into consideration. In the absence of bromide, formaldehyde contributed 96–97% cytotoxicity and 92–95% genotoxicity to HepG2 cells among the detected known byproducts, while acetaldehyde, propionaldehyde, and glyoxal had little toxicity. Both formaldehyde and dibromoacetonitrile drove toxicity among the known byproducts when bromide was present. Toxicity assays in HepG2 cells showed that when secondary effluents contained no bromide, the cytotoxicity of the nonvolatile organic fraction (NVOF) was reduced by 56–70%, and genotoxicity was completely removed after ozonation. However, the formed aldehydes (volatile organic fraction, VOF) led to increased overall toxicity. In the presence of bromide, compared with the secondary effluent, ozonation increased the cytotoxicity of the NVOFBr from 3.4–4.0 mg phenol/L to 10.3–13.9 mg phenol/L, possibly due to the formation of organic bromine. In addition, considering the toxicity of VOFBr (VOF in the presence of bromide, including aldehydes, tribromomethane, etc.), the overall cytotoxicity and genotoxicity became much higher than those of the secondary effluent. Although bromate had a limited impact on cytotoxicity and genotoxicity, it caused an increase in oxidative stress in HepG2 cells. Therefore, when taking full account of nonvolatile, volatile, and inorganic fractions, ozonation generally increases the toxicity of wastewater.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Toxicity of Ozonated Wastewater to HepG2 Cells: Taking Full Account of Nonvolatile, Volatile, and Inorganic Byproducts

Yang

et al. 2021

Environ. Sci. Technol.

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“…Citing a few examples, Rossow et al presented a droplet microfluidic templating with bio-orthogonal thiol-ene click reactions to prepare cell-laden microgel particles for cell encapsulation [ 18 ]. Zheng and colleagues proposed an integrated microfluidic chip to prepare heterogeneous cell-encapsulated alginate microcapsules coupled to mass spectrometry, probing the drug resistance in a tumor-endothelial co-culture model [ 19 ]. Although microfluidics offers various advantages, such as the reduction of sample volumes and minute control over the shape and size of microbeads, the quantity of production remains challenging.…”

Section: Introductionmentioning

confidence: 99%

DoE Analysis of Approaches for Hydrogel Microbeads’ Preparation by Millifluidic Methods

et al. 2020

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Hydrogel microbeads hold great promise for immune-protective cell transplants and in vitro studies. Millifluidic generation of hydrogel microbeads is a highly efficient and reproducible approach enabling a mass production. This paper illustrates the preparation and characterization of highly controlled and reproducible microbeads made by different types of hydrogel using millifluidic approaches. The optimization of the process was made by a design of experiments (DoE) approach. The microbeads’ large-scale production can be potentially used for single cells or clusters encapsulation.

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“…70 Tumor cells and endothelial cells were co-cultured in gel microspheres, further revealing the metabolism of paclitaxel. 71…”

Section: Analytical Sciencesmentioning

confidence: 99%

Cell Analysis on Microfluidics Combined with Mass Spectrometry

Zhang

Lin

2020

ANAL. SCI.

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Cell analysis is of great significance for the exploration of human diseases and health. However, there are not many techniques for high-throughput cell analysis in the simulated cell microenvironment. The high designability of the microfluidic chip enables multiple kinds of cells to be co-cultured on the chip, with other functions such as sample preprocessing and cell manipulation. Mass spectrometer (MS) can detect a large number of biomolecules without labelling. Therefore, microfluidic chip coupled with MS has been a major branch of cell analysis during the past decades. Here, we concisely introduce various microfluidic devices coupling with MS used for cell analysis. The main functions of microfluidic devices were first described, followed with different interfaces to different types of MS. Then, their various applications in cell analysis were highlighted, with an emphasis on cell metabolism, drug screening, and signal transduction. Current limitations and prospective trends of microfluidics coupling with MS are discussed at the end.

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Imitation of drug metabolism in cell co-culture microcapsule model using a microfluidic chip platform coupled to mass spectrometry

Cited by 42 publications

References 20 publications

Toxicity of Ozonated Wastewater to HepG2 Cells: Taking Full Account of Nonvolatile, Volatile, and Inorganic Byproducts

Toxicity of Ozonated Wastewater to HepG2 Cells: Taking Full Account of Nonvolatile, Volatile, and Inorganic Byproducts

DoE Analysis of Approaches for Hydrogel Microbeads’ Preparation by Millifluidic Methods

Cell Analysis on Microfluidics Combined with Mass Spectrometry

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