Imidacloprid biodegradation using novel bacteria Tepidibacillus decaturensis strain ST1 in batch and in situ microcosm study

Tiwari, Sonam; Tripathi, Pranjal; Mohan, Devendra; Singh, Ram Sharan

doi:10.1007/s11356-022-24779-8

Cited by 11 publications

(3 citation statements)

References 44 publications

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“…PC-21 isolated from soil was reported to degrade 37–58% of IMI in full-strength tryptic soy broth [ 59 ]. Tiwari et al [ 60 ] reported use of the Tepidibacillus decaturensis strain ST1 resulted in the degradation of around 77.5% and 85% of IMI in sterile and unsterile soils, respectively, within 45 days. Madhuban et al [ 61 ] investigated the biodegradation of IMI by an aerobic bacterium capable of digesting IMI isolated from an agricultural field soil using enrichment culture.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Biofilm-Mediated Biodegradation of Pesticides and Dye-Contaminated Effluents Using Bacterial Biofilms

Liaqat,

Khalid,

Rubab

et al. 2023

Microorganisms

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Overuse of pesticides in agricultural soil and dye-polluted effluents severely contaminates the environment and is toxic to animals and humans making their removal from the environment essential. The present study aimed to assess the biodegradation of pesticides (cypermethrin (CYP) and imidacloprid (IMI)), and dyes (malachite green (MG) and Congo red (CR)) using biofilms of bacteria isolated from pesticide-contaminated soil and dye effluents. Biofilms of indigenous bacteria, i.e., Bacillus thuringiensis 2A (OP554568), Enterobacter hormaechei 4A (OP723332), Bacillus sp. 5A (OP586601), and Bacillus cereus 6B (OP586602) individually and in mixed culture were tested against CYP and IMI. Biofilms of indigenous bacteria i.e., Lysinibacillus sphaericus AF1 (OP589134), Bacillus sp. CF3 (OP589135) and Bacillus sp. DF4 (OP589136) individually and in mixed culture were tested for their ability to degrade dyes. The biofilm of a mixed culture of B. thuringiensis + Bacillus sp. (P7) showed 46.2% degradation of CYP compared to the biofilm of a mixed culture of B. thuringiensis + E. hormaechei + Bacillus sp. + B. cereus (P11), which showed significantly high degradation (70.0%) of IMI. Regarding dye biodegradation, a mixed culture biofilm of Bacillus sp. + Bacillus sp. (D6) showed 86.76% degradation of MG, which was significantly high compared to a mixed culture biofilm of L. sphaericus + Bacillus sp. (D4) that degraded only 30.78% of CR. UV–VIS spectroscopy revealed major peaks at 224 nm, 263 nm, 581 nm and 436 nm for CYP, IMI, MG and CR, respectively, which completely disappeared after treatment with bacterial biofilms. Fourier transform infrared (FTIR) analysis showed the appearance of new peaks in degraded metabolites and disappearance of a peak in the control spectrum after biofilm treatment. Thin layer chromatography (TLC) analysis also confirmed the degradation of CYP, IMI, MG and CR into several metabolites compared to the control. The present study demonstrates the biodegradation potential of biofilm-forming bacteria isolated from pesticide-polluted soil and dye effluents against pesticides and dyes. This is the first report demonstrating biofilm-mediated bio-degradation of CYP, IMI, MG and CR utilizing soil and effluent bacterial flora from Multan and Sheikhupura, Punjab, Pakistan.

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Section: Discussionmentioning

confidence: 99%

In Vitro Biofilm-Mediated Biodegradation of Pesticides and Dye-Contaminated Effluents Using Bacterial Biofilms

Liaqat,

Khalid,

Rubab

et al. 2023

Microorganisms

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“…strain DF-1 with soil resulted in complete nitenpyram insecticide degradation in both sterile and non-sterilized soil within 14 days of incubation [94]. Similarly, soil inoculation of Tepidibacillus decaturensis strain ST1 degraded 200 mg kg −1 of imidacloprid insecticides completely with a half-life of 12.95 days in non-sterile soil and 18.77 days in sterile soil [95]. Therefore, proper inoculation techniques lead to the development of higher root colonization of degradable microbes, which can provide a niche for pesticide-degrading microbes in the plant microbiome.…”

Section: Phytomicrobiome and Pesticide Biodegradationmentioning

confidence: 92%

Phytomicrobiomes: A Potential Approach for Sustainable Pesticide Biodegradation

Salam,

Mahmood,

Asghar

et al. 2024

Applied Sciences

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Globally, pest-induced crop losses ranging from 20% to 40% have spurred the extensive use of pesticides, presenting a double-edged sword that threatens not only human health but also our environment. Amidst various remediation techniques, bioremediation stands out as a compelling and eco-friendly solution. Recently, the phytomicrobiome has garnered increasing attention as endophytic microbes, colonizing plants from their roots, not only foster plant growth but also enhance the host plant’s resilience to adverse conditions. Given the persistent demand for high crop yields, agricultural soils often bear the burden of pesticide applications. Biodegradation, the transformation of complex pesticide compounds into simpler forms through the activation of microbial processes and plant-based enzymatic systems, emerges as a pivotal strategy for restoring soil health. Manipulating the phytomicrobiome may emerge as a viable solution for this purpose, offering a native metabolic pathway that catalyzes pollutant degradation through enzymatic reactions. This review delves into the pivotal role of phytomicrobiomes in the degradation of diverse pesticides in soil. It explores contemporary innovations and paves the way for discussions on future research directions in this promising field.

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“…After screening, on the basis of batch studies, two most efficient isolates were subjected to characterization and identification. Furthermore, a consortium was prepared using the two isolates and used for further degradation studies as mentioned in a previous study . Using MEGA software (version 10.2.5), a phylogenetic tree was constructed, and the 16S rRNA sequences of the isolates were assigned a GenBank accession number.…”

Section: Methodsmentioning

confidence: 99%

Bioremediation of Imidacloprid in a Stirred Tank Reactor Using Bacterial Consortium: Kinetic Analysis and Toxicity Assessment

Tiwari

Tripathi

Mohan

et al. 2023

Ind. Eng. Chem. Res.

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Imidacloprid is an insecticide of systemic nature that exhibits an adverse impact on several non-target organisms. In the present study, potential bacteria have been isolated and used for biodegradation of imidacloprid in batch studies and stirred tank reactor. The optimum process condition for degradation has been found to be at pH 7, a temperature of 35 °C, and a shaking speed of 150 rpm. Maximum degradation of 78% has been achieved by the bacterial consortium in the batch study, while, in the reactor, it increases to 90%. Kinetics analysis suggests that the Teisser model best fits the experimental data, with a correlation coefficient (R 2) of 0.98. Ecotoxicological assessment using luminescent bacteria reveals 40% and 90% inhibition in luminescence in the case of untreated sample upon exposure for 30 min and 24 h, respectively, while the values obtained in the case of treated samples are 9% and 29%, respectively. Cytotoxicity assessment indicates 60% reduction in cell proliferation in untreated samples and <10% cell proliferation inhibition in treated samples, indicating a considerable decrease in toxicity post-bacterial treatment.

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Imidacloprid biodegradation using novel bacteria Tepidibacillus decaturensis strain ST1 in batch and in situ microcosm study

Cited by 11 publications

References 44 publications

In Vitro Biofilm-Mediated Biodegradation of Pesticides and Dye-Contaminated Effluents Using Bacterial Biofilms

In Vitro Biofilm-Mediated Biodegradation of Pesticides and Dye-Contaminated Effluents Using Bacterial Biofilms

Phytomicrobiomes: A Potential Approach for Sustainable Pesticide Biodegradation

Bioremediation of Imidacloprid in a Stirred Tank Reactor Using Bacterial Consortium: Kinetic Analysis and Toxicity Assessment

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