IME1, a positive regulator gene of meiosis in S. cerevisiae

Kassir, Yona; Granot, David; Simchen, Giora

doi:10.1016/0092-8674(88)90427-8

Cited by 295 publications

(293 citation statements)

References 24 publications

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“…Plasmid pYIl 13 ) is a 2-,um-based multicopy plasmid carrying LEU2 and URA3, and used to obtain prototrophic strains, since the sporulation efficiency is greatly reduced when auxotrophic cells are shifted from culture on SD plus supplements into Spo medium. Plasmids pHKI The strains AM1205-9B and 419-G2 3 ) were kindly provided by A. Mitchell and G. Simchen, respectively. Diploid strains used in this study were constructed by mating the haploid strains listed.…”

Section: Methodsmentioning

confidence: 99%

Nutritional Regulation of Meiosis-specific Gene Expression in Saccharomyces cerevisiae

Kawaguchi

Yoshida

Yamashita

1992

Bioscience, Biotechnology, and Biochemistry

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Section: Methodsmentioning

confidence: 99%

Nutritional Regulation of Meiosis-specific Gene Expression in Saccharomyces cerevisiae

Kawaguchi

Yoshida

Yamashita

1992

Bioscience, Biotechnology, and Biochemistry

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“…A promising candidate locus that we predicted should have antagonistic pleiotropic effects on sporulation and pseudohyphal growth is RME1 (Repressor of Meiosis). Rme1p is a zinc-finger transcription factor involved in inhibiting meiosis by repressing the expression of IME1, a meiotic initiator (39,40). Allelic variation in the promoter region of RME1 has been shown to affect sporulation ability (41)(42)(43).…”

Section: Variation In Rme1 Expression Contributes To the Phenotypic Tmentioning

confidence: 99%

Outcrossing, mitotic recombination, and life-history trade-offs shape genome evolution in Saccharomyces cerevisiae

Magwene

Kayıkçı

Granek

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

159

179

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We carried out a population genomic survey of Saccharomyces cerevisiae diploid isolates and find that many budding yeast strains have high levels of genomic heterozygosity, much of which is likely due to outcrossing. We demonstrate that variation in heterozygosity among strains is correlated with a life-history tradeoff that involves how readily yeast switch from asexual to sexual reproduction under nutrient stress. This trade-off is reflected in a negative relationship between sporulation efficiency and pseudohyphal development and correlates with variation in the expression of RME1, a transcription factor with pleiotropic effects on meiosis and filamentous growth. Selection for alternate lifehistory strategies in natural versus human-associated environments likely contributes to differential maintenance of genomic heterozygosity through its effect on the frequency that yeast lineages experience sexual cycles and hence the opportunity for inbreeding. In addition to elevated levels of heterozygosity, many strains exhibit large genomic regions of loss-of-heterozygosity (LOH), suggesting that mitotic recombination has a significant impact on genetic variation in this species. This study provides new insights into the roles that both outcrossing and mitotic recombination play in shaping the genome architecture of Saccharomyces cerevisiae. This study also provides a unique case where stark differences in the genomic distribution of genetic variation among individuals of the same species can be largely explained by a lifehistory trade-off.T he frequency of sex and the nature of breeding systems have a profound effect on genome variation and evolution. For example, inbred populations have an increased frequency of homozygous genotypes (1), lower effective rates of recombination (2), and smaller effective population sizes relative to outcrossed populations with the same number of individuals (3). Likewise, clonal populations are expected to exhibit high levels of heterozygosity coupled with increased allelic diversity but decreased genotypic diversity relative to sexual populations (4).The budding yeast Saccharomyces cerevisiae is one of the best studied model organisms, but relatively little is known about the importance of sexual versus asexual reproduction and inbreeding versus outcrossing in shaping genome evolution in this species. One recent study estimated that outcrossing occurs approximately once every 50,000 generations in S. cerevisiae (5), but low rates of outcrossing do not preclude the possibility that outcrossing has an important impact on genetic variation. Studies of the closely related yeast Saccharomyces paradoxus suggest that sexual cycles are rare relative to asexual cycles and that when sex does occur it primarily involves inbreeding (6, 7). However, S. paradoxus exhibits distinctly different intra-and interpopulation patterns of variation than does S. cerevisiae (8), and hence these findings may not be generalizable across the Saccharomyces genus.Patterns of heterozygosity are an important indica...

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“…The first wave includes the early meiotic genes, which function specifically in premeiotic S phase and in meiotic prophase Primig et al, 2000). Expression of early meiotic genes depends upon the transcriptional activator Ime1 (Kassir et al, 1988;Smith et al, 1990). The transition from meiotic prophase to the first meiotic division relies on a meiosis-specific transcriptional activator, Ndt80, as well as Cdc28-Clb1 kinase activity (Xu et al, 1995;Benjamin et al, 2003;Carlile and Amon 2008).…”

Section: Introductionmentioning

confidence: 99%

Cdc7-Dbf4 RegulatesNDT80Transcription as Well as Reductional Segregation during Budding Yeast Meiosis

Wan

Rosebrock

et al. 2008

MBoC

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In budding yeast, as in other eukaryotes, the Cdc7 protein kinase is important for initiation of DNA synthesis in vegetative cells. In addition, Cdc7 has crucial meiotic functions: it facilitates premeiotic DNA replication, and it is essential for the initiation of recombination. This work uses a chemical genetic approach to demonstrate that Cdc7 kinase has additional roles in meiosis. First, Cdc7 allows expression of NDT80, a meiosis-specific transcriptional activator required for the induction of genes involved in exit from pachytene, meiotic progression, and spore formation. Second, Cdc7 is necessary for recruitment of monopolin to sister kinetochores, and it is necessary for the reductional segregation occurring at meiosis I. The use of the same kinase to regulate several distinct meiosis-specific processes may be important for the coordination of these processes during meiosis. INTRODUCTIONSexually reproducing, eukaryotic cells use two types of cell division: mitosis and meiosis. In mitosis, chromosomes are replicated and the resulting sister chromatids are then segregated to produce two genetically identical daughter cells. In contrast, meiosis generates haploid gametes from diploid cells by having one round of DNA replication followed by two rounds of chromosome segregation. A unique feature of meiosis is that pairs of homologous sister chromatids are pulled to opposite poles (reductional segregation) at the first meiotic division (meiosis I). The second meiotic division (meiosis II) resembles mitosis in that sister chromatids are segregated to opposite poles (called equational segregation). In budding yeast, meiotic chromosome behavior arises from the interplay between meiosis-specific proteins and proteins involved in mitosis (Marston and Amon 2004;Wan et al., 2008).Progression through meiosis relies on transcriptional cascades regulated in part by protein kinases (Vershon and Pierce 2000). Microarray analysis has identified several temporal waves of transcription. The first wave includes the early meiotic genes, which function specifically in premeiotic S phase and in meiotic prophase Primig et al., 2000). Expression of early meiotic genes depends upon the transcriptional activator Ime1 (Kassir et al., 1988;Smith et al., 1990). The transition from meiotic prophase to the first meiotic division relies on a meiosis-specific transcriptional activator, Ndt80, as well as Cdc28-Clb1 kinase activity (Xu et al., 1995;Benjamin et al., 2003;Carlile and Amon 2008). Ndt80 binds to a sequence motif called the middle sporulation element, or MSE, upstream of genes in the second wave of transcription. These genes are called middle sporulation genes, and they are necessary for exit from pachytene, meiotic progression, and spore morphogenesis (Hepworth et al., 1995(Hepworth et al., , 1998Allers and Lichten 2001). Cells lacking NDT80 arrest at pachytene with duplicated, but unseparated, spindle pole bodies (Xu et al., 1995). One of the genes regulated by Ndt80 is CLB1, which, together with Cdc28 kinase, promotes executio...

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IME1, a positive regulator gene of meiosis in S. cerevisiae

Cited by 295 publications

References 24 publications

Nutritional Regulation of Meiosis-specific Gene Expression in Saccharomyces cerevisiae

Nutritional Regulation of Meiosis-specific Gene Expression in Saccharomyces cerevisiae

Outcrossing, mitotic recombination, and life-history trade-offs shape genome evolution in Saccharomyces cerevisiae

Cdc7-Dbf4 RegulatesNDT80Transcription as Well as Reductional Segregation during Budding Yeast Meiosis

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