Imbibition of wheat seeds: Application of image analysis

Lev, Jakub; Blahovec, J.

doi:10.1515/intag-2016-0072

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…With the "Blue" channel and fixed threshold (140/255), Lev and Blahovec (2017) obtained the effective segmentation of wheat seeds during imbibition by maintaining controlled light conditions throughout the experiment. In the present study, with the use of the dark chamber over the scanner, no environmental effects of saturation or luminosity were observed during the entire image acquisition period.…”

Section: Resultsmentioning

confidence: 99%

Image-based modelling of the effect of s-metolachlor plus atrazine on the soaking kinetics of maize seeds

Santos¹,

Silva²,

Santos³

et al. 2022

Int. Agrophys.

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A b s t r a c t. Pre-emergent herbicides can have negative effects on maize seeds. The objective of this study was to model seed soaking curves through the processing of red-green-blue imagery of maize seeds under the influence of concentrations of s-metolachlor + atrazine on both the soaking kinetics and primary root emission. Seeds were placed to soak for 114 h in Petri dishes containing aqueous solutions of a herbicide containing s-metolachlor (290 g l -1 ) + atrazine (370 g l -1 ) with the following concentrations: 0% (water only), 2, 5, 10, 20 and 50%, based on the recommended dose (4.0 l of the commercial product per hectare). The images were systematically taken from a flatbed scanner with artificial light control. The red excess index was adapted to improve image segmentation. From the binary masks applied, the soaking curves for each herbicide concentration were obtained using estimates of seed intumescence over time. The soaking curves were described by fitting Peleg's model. The herbicide concentration has significant effects on both the absorption rate and primary root emission; the absorption rate was reduced by 50%. A concentration of s-metolachlor (290 g l -1 ) + atrazine (370 g l -1 ) in aqueous solution that is above 20% can fully inhibit seed germination.K e y w o r d s: imbibition curve; Zea mays; herbicide; image processing.

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Section: Resultsmentioning

confidence: 99%

Image-based modelling of the effect of s-metolachlor plus atrazine on the soaking kinetics of maize seeds

Santos¹,

Silva²,

Santos³

et al. 2022

Int. Agrophys.

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“…The detected outlines were used for the selection of the kernel regions in the LI (the position of the kernels is identical in both images LI and CI). These regions were then rotated so that its major axis was parallel to the x-axis (Gonzalez and Woods, 2002;Lev and Blahovec, 2017). The kernel regions were converted to the grey scale.…”

Section: Methodsmentioning

confidence: 99%

Detection of the entrance of Lugol’s solution into the aleurone layer during germination

Lev¹,

Kameneva²,

Blahovec³

2019

Int. Agrophys.

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A b s t r a c t. The movement of water into dry kernels is a critical step in germination, preharvest sprouting, and in the expression of dormancy. The soaking of kernels in Lugol's solution allows for water uptake visualization by staining the cellular membranes and starch tissue. It is possible to recognize a stained aleurone layer during germination via image analysis (without cutting). A new method for the detection of Lugol's solution in the aleurone layer of four wheat varieties is presented. The first entrance of the solution into the aleurone layer was detected. The rate at which the solution spread through the aleurone layer was also demonstrated. It was determined that the initial entrance time of the solution into the aleurone layer was different for the individual varieties (1.5-6 h) whereas the rate at which it spread in the aleurone layer was similar for the tested varieties and takes approximately 0.2-0.35 mm h -1 . It was established that there is a close correlation between the solution speed in the aleurone layer and the mass increase during solution uptake for 3 out of 4 varieties. K e y w o r d s: imbibition, wheat, image analysis, germination phases, method

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“…Biochemical analysis is widely used for monitoring seeds during germination, but it is often focused on the final phase of germination (the start of seedling growth), and that is paid the most attention. However, the initial phase of germination, imbibition, also exhibits interesting behavior [18]. The imbibition phase is divided into three defined parts [19].…”

Section: Introductionmentioning

confidence: 99%

“…The second one, also known as main imbibition, consists of spatial expansion caused by water adsorption in the other parts of the seed. The third one is the growth phase, in which radicle protrusion commences, followed by continued root and seedling growth [18,20]. The results of imbibition include a rapid increase in mitochondrial activity and high glyoxysomal activity.…”

Section: Introductionmentioning

confidence: 99%

Effect of Fluoride on Germination, Early Growth and Antioxidant Enzymes Activity of Three Winter Wheat (Triticum aestivum L.) Cultivars

et al. 2020

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This paper assesses the impact of sodium fluoride on the morphological parameters and activity of catalase and peroxidase during the germination and root growth phases of three winter wheat cultivars: Tobak, Dalewar, and Arkadia. During examination, the seeds were placed on plastic Petri dishes with an NaF solution at concentrations of 0 (control), 2.5, 5.0, 8.0, and 10.0 mmol dm−3. The obtained results have shown a decrease in germination, inhibition of root growth, and inhibition of catalase activity, both in the embryos and roots of all tested winter wheat cultivars. The observed effects have been strengthened with the increase of the fluoride concentration. However, the effect of NaF on the peroxidase activity has been dependent on the wheat cultivar. It is difficult to state unequivocally which of the tested winter wheat cultivars has been characterized by the highest sensitivity to fluoride. An η2 analysis has confirmed that the NaF concentration has a greater effect than the winter wheat cultivar on the activity of the determined antioxidant enzymes.

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Imbibition of wheat seeds: Application of image analysis

Cited by 11 publications

References 18 publications

Image-based modelling of the effect of s-metolachlor plus atrazine on the soaking kinetics of maize seeds

Image-based modelling of the effect of s-metolachlor plus atrazine on the soaking kinetics of maize seeds

Detection of the entrance of Lugol’s solution into the aleurone layer during germination

Effect of Fluoride on Germination, Early Growth and Antioxidant Enzymes Activity of Three Winter Wheat (Triticum aestivum L.) Cultivars

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