A series of experiments were conducted to observe water transport in wood during wetting using a micro-CT combined with scanning, reconstruction, and grey analysis technology. A comprehensive discussion was conducted to explore the characteristics of water transport and moisture distribution caused by local pore structure and properties in the wood sample during wetting. For wetting course of the wood immersed in water, water transport exhibits three stages. At the beginning, the dense region of the wood was wetted and contained some water which was unable to transfer into the sparse region because of the larger capillary force induced by the dense structure. Secondly, as water penetrates the wood, the sparse region contained much more water than the dense region. At this stage, the dense region is unsaturated. Finally, water transfers into both dense and sparse regions and gradually saturates them.