Imbalance of intestinal microbial homeostasis caused by acetamiprid is detrimental to resistance to pathogenic bacteria in Bombyx mori

Li, Fanchi; Li, Mengxue; Zhu, Qingyu; Mao, Tingting; Dai, Minli; Ye, Wentao; Bian, Dandan; Su, Wujie; Feng, Piao; Ren, Yuying; Sun, Hongjuan; Wei, Jing; Li, Bing

doi:10.1016/j.envpol.2021.117866

Cited by 13 publications

(4 citation statements)

References 26 publications

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“…Based on these observations, we found various DE defensins and lysozymes under rearing conditional changes in the R. pedestris gut, including four defensin members and nine lysozyme members, and moreover, seven lncRNAs and two miRNAs in the LyzI-4-related ceRNA network were shown to differential expression by antibiotic treatment and different feeding treatments. In agreement with our investigations, an imbalance in gut microbial homeostasis affected AMP expression in B. mori under Enterobacter cloacae infection by acetamiprid feeding (Li F. C. et al, 2021). For the fall armyworm (FAW), Spodoptera frugiperda, diets and different survival conditions impacted its gut microbiota and immune system, indicating significant changes in lysozymes, attacins and cecropins genes, and gut microbiome communities between captive and wild samples (Zheng et al, 2023).…”

Section: Discussionsupporting

confidence: 89%

The ncRNA-mediated regulatory networks of defensins and lysozymes in Riptortus pedestris: involvement in response to gut bacterial disturbances

Ren,

Fu,

Dong

et al. 2024

Front. Microbiol.

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Insects depend on humoral immunity against intruders through the secretion of antimicrobial peptides (AMPs) and immune effectors via NF-κB transcription factors, and their fitness is improved by gut bacterial microbiota. Although there are growing numbers of reports on noncoding RNAs (ncRNAs) involving in immune responses against pathogens, comprehensive studies of ncRNA-AMP regulatory networks in Riptortus pedestris, which is one of the widely distributed pests in East Asia, are still not well understood under feeding environmental changes. The objective of this study employed the whole-transcriptome sequencing (WTS) to systematically identify the lncRNAs (long noncoding RNA) and circRNAs (circular RNA) and to obtain their differential expression from the R. pedestris gut under different feeding conditions. Functional annotation indicated that they were mainly enriched in various biological processes with the GO and KEGG databases, especially in immune signaling pathways. Five defensin (four novel members) and eleven lysozyme (nine novel members) family genes were identified and characterized from WTS data, and meanwhile, phylogenetic analysis confirmed their classification. Subsequently, the miRNA–mRNA interaction network of above two AMPs and lncRNA-involved ceRNA (competing endogenous RNA) regulatory network of one lysozyme were predicted and built based on bioinformatic prediction and calculation, and the expression patterns of differentially expressed (DE) defensins, and DE lysozymes and related DE ncRNAs were estimated and selected among all the comparison groups. Finally, to integrate the analyses of WTS and previous 16S rRNA amplicon sequencing, we conducted the Pearson correlation analysis to reveal the significantly positive or negative correlation between above DE AMPs and ncRNAs, as well as most changes in the gut bacterial microbiota at the genus level of R. pedestris. Taken together, the present observations provide great insights into the ncRNA regulatory networks of AMPs in response to rearing environmental changes in insects and uncover new potential strategies for pest control in the future.

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Section: Discussionsupporting

confidence: 89%

The ncRNA-mediated regulatory networks of defensins and lysozymes in Riptortus pedestris: involvement in response to gut bacterial disturbances

Ren,

Fu,

Dong

et al. 2024

Front. Microbiol.

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“…Li et al found that vaccination with sublethal doses of acetamiprid activates the Duox-ROS system and induces ROS accumulation, leading to intestinal dysbiosis and the translocation of Pseudomonas and Staphylococcus to the hemolymph. Acetamiprid-treated B. mori larvae inoculated with E. cloacae significantly reduced survival and body weight [ 73 ]. The expression of peritrophic membrane protein-related genes BmCBP-02 , BmPM-41 , BmPM-43 , and BmCDA7, as well as the toll signaling pathway-related genes Bmcactus , Bmspatzle , and Bmrel , was significantly down-regulated in the gut of B. mori following exposure to phoxim, leading to B. mori’s susceptibility to E. cloacae [ 74 ].…”

Section: Negative Effects Of Gut Microbes On the Hostmentioning

confidence: 99%

Research Progresses on the Function and Detection Methods of Insect Gut Microbes

Chang

et al. 2023

Microorganisms

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The insect gut is home to an extensive array of microbes that play a crucial role in the digestion and absorption of nutrients, as well as in the protection against pathogenic microorganisms. The variety of these gut microbes is impacted by factors such as age, diet, pesticides, antibiotics, sex, and caste. Increasing evidence indicates that disturbances in the gut microbiota can lead to compromised insect health, and that its diversity has a far-reaching impact on the host’s health. In recent years, the use of molecular biology techniques to conduct rapid, qualitative, and quantitative research on the host intestinal microbial diversity has become a major focus, thanks to the advancement of metagenomics and bioinformatics technologies. This paper reviews the main functions, influencing factors, and detection methods of insect gut microbes, in order to provide a reference and theoretical basis for better research utilization of gut microbes and management of harmful insects.

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“…Under normal physiological conditions, the intestinal microbial population exhibits a stable fluctuation range. Drastic changes result in an imbalance in the microbial homeostasis of the microbiota, directly affecting the growth, development, reproduction and other biological activities of insects [ 8 , 9 ]. Studies have shown a decrease in the intestinal microbiota diversity and richness of silkworms fed with lettuce, disrupting the healthy intestinal microflora’s homeostasis, leading to a reduced digestive enzyme activity and hindering the growth of silkworms [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Homeostatic Regulation of the Duox-ROS Defense System: Revelations Based on the Diversity of Gut Bacteria in Silkworms (Bombyx mori)

Shu

Guo

Tian

et al. 2023

IJMS

Self Cite

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The Duox-ROS defense system plays an important role in insect intestinal immunity. To investigate the role of intestinal microbiota in Duox-ROS regulation herein, 16S rRNA sequencing technology was utilized to compare the characteristics of bacterial populations in the midgut of silkworm after different time-periods of treatment with three feeding methods: 1–4 instars artificial diet (AD), 1–4 instars mulberry leaf (ML) and 1–3 instars artificial diet + 4 instar mulberry leaf (TM). The results revealed simple intestinal microbiota in the AD group whilst microbiota were abundant and variable in the ML and TM silkworms. By analyzing the relationship among intestinal pH, reactive oxygen species (ROS) content and microorganism composition, it was identified that an acidic intestinal environment inhibited the growth of intestinal microbiota of silkworms, observed concurrently with low ROS content and a high activity of antioxidant enzymes (SOD, TPX, CAT). Gene expression associated with the Duox-ROS defense system was detected using RT-qPCR and identified to be low in the AD group and significantly higher in the TM group of silkworms. This study provides a new reference for the future improvement of the artificial diet feeding of silkworm and a systematic indicator for the further study of the relationship between changes in the intestinal environment and intestinal microbiota balance caused by dietary alterations.

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Imbalance of intestinal microbial homeostasis caused by acetamiprid is detrimental to resistance to pathogenic bacteria in Bombyx mori

Cited by 13 publications

References 26 publications

The ncRNA-mediated regulatory networks of defensins and lysozymes in Riptortus pedestris: involvement in response to gut bacterial disturbances

The ncRNA-mediated regulatory networks of defensins and lysozymes in Riptortus pedestris: involvement in response to gut bacterial disturbances

Research Progresses on the Function and Detection Methods of Insect Gut Microbes

Homeostatic Regulation of the Duox-ROS Defense System: Revelations Based on the Diversity of Gut Bacteria in Silkworms (Bombyx mori)

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