Imbalance of intermediate filament component keratin 14 contributes to increased stress signalling in epidermolysis bullosa simplex

Wagner, Martin; Trost, Andrea; Hintner, Helmut; Bauer, Johann; Önder, Kamil

doi:10.1111/exd.12112

Cited by 17 publications

(21 citation statements)

References 16 publications

(12 reference statements)

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“…EB Simplex and Dystrophic EB are two common types of EB because of mutations in the gene of keratins 5 or 14 (EB Simplex) and type VII collagen (Dystrophic EB) (18,(23)(24)(25).…”

Section: Discussionmentioning

confidence: 99%

Wool‐derived keratin stimulates human keratinocyte migration and types IV and VII collagen expression

Tang

Ollague

Kelly

et al. 2012

Experimental Dermatology

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Keratinocyte migration is essential for wound repair. Keratin-based products have recently shown stimulatory effects on wound repair. This study was to test the cellular response to wool-derived oxidized keratin in wound healing to further understand the biological mechanisms underlying observed clinical benefits of keratin-based products as wound treatments. In vitro scratch migration assays examined the effects of oxidized keratin on the migration of human skin keratinocytes. Western blotting analysis determined the effects on the marker protein expression of type IV and type VII collagens and keratin 17. We found wool-derived oxidized keratin promoted keratinocyte migration and induced protein expression of type IV and type VII collagens, but not keratin 17. The data suggest that the beneficial effects of keratin-based treatment in wounds may be related to its positive effects on re-epithelialization via stimulating keratinocyte migration and production of basement membrane proteins of types IV and VII collagens.

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“…EB Simplex and Dystrophic EB are two common types of EB because of mutations in the gene of keratins 5 or 14 (EB Simplex) and type VII collagen (Dystrophic EB) (18,(23)(24)(25).…”

Section: Discussionmentioning

confidence: 99%

Wool‐derived keratin stimulates human keratinocyte migration and types IV and VII collagen expression

Tang

Ollague

Kelly

et al. 2012

Experimental Dermatology

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“…Some features of the K14 mutations phenotype, however, seem to suggest that there are possible non-mechanical mechanisms involved. The R125P K14 mutation elicits JNK signaling 22 . Mutant K14 increases TNFα secretion and sensitivity 23 .…”

Section: Keratins Mechanical Is Not Mechanisticmentioning

confidence: 99%

Multiple roles for keratin intermediate filaments in the regulation of epithelial barrier function and apico-basal polarity

2016

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As multicellular organisms evolved a family of cytoskeletal proteins, the keratins (types I and II) expressed in epithelial cells diversified in more than 20 genes in vertebrates. There is no question that keratin filaments confer mechanical stiffness to cells. However, such a number of genes can hardly be explained by evolutionary advantages in mechanical features. The use of transgenic mouse models has revealed unexpected functional relationships between keratin intermediate filaments and intracellular signaling. Accordingly, loss of keratins or mutations in keratins that cause or predispose to human diseases, result in increased sensitivity to apoptosis, regulation of innate immunity, permeabilization of tight junctions, and mistargeting of apical proteins in different epithelia. Precise mechanistic explanations for these phenomena are still lacking. However, immobilization of membrane or cytoplasmic proteins, including chaperones, on intermediate filaments (“scaffolding”) appear as common molecular mechanisms and may explain the need for so many different keratin genes in vertebrates.

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“…These differences suggest that the mechanism of action of TMAO may be dependent on the mutations of the affected cells (7,8) or on the concentration of TMAO (5).…”

Section: Resultsmentioning

confidence: 99%

Reduction in keratin aggregates in epidermolysis bullosa simplex keratinocytes after pretreatment with trimethylamine N‐oxide

Bchetnia

Lacroix

Farez

et al. 2015

Experimental Dermatology

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Imbalance of intermediate filament component keratin 14 contributes to increased stress signalling in epidermolysis bullosa simplex

Cited by 17 publications

References 16 publications

Wool‐derived keratin stimulates human keratinocyte migration and types IV and VII collagen expression

Wool‐derived keratin stimulates human keratinocyte migration and types IV and VII collagen expression

Multiple roles for keratin intermediate filaments in the regulation of epithelial barrier function and apico-basal polarity

Reduction in keratin aggregates in epidermolysis bullosa simplex keratinocytes after pretreatment with trimethylamine N‐oxide

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