Imbalance of Caveolin‐1 and eNOS Expression in the Pulmonary Vasculature of Experimental Diaphragmatic Hernia

Hofmann, Alejandro D.; Gosemann, Jan‐Hendrik; Takahashi, Toshiaki; Friedmacher, Florian; Duess, Johannes W.; Puri, Prem

doi:10.1002/bdrb.21117

Cited by 18 publications

(21 citation statements)

References 36 publications

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“…Pregnant mice and rats were exposed to nitrofen during Day 9 (D9) of gestation. Nitrofen exposure resulted in a high rate of CDH with associated PH and pulmonary vasculature abnormalities in fetuses sacrificed on D21, strikingly similar to the human situation (Hofmann et al, 2014). They observed that pulmonary Cav-1 gene expression and protein levels were significantly downregulated, whereas eNOS expression levels were dramatically upregulated in these CDH animal models.…”

Section: Caveolae and Caveolins In Human Diseasementioning

confidence: 53%

“…3). Despite the fact that currently existing caveolin KO mice, whether it is a single gene or double gene (Cav-1 and Cav-3) KO, are viable and fertile (Park et al, 2002), there has been an increasing body of evidence that Cav-1 is also implicated in birth defects of lung and brain (Hofmann et al, 2014; Yang et al, 2015). Most interestingly, evidence for caveolae/Cav-1 functions in stem cell-mediated tissue repair/regeneration and wound healing is plentiful (see review by Baker and Tuan, 2013); however, more sophisticated approaches to delineate the role of caveolae/caveolin in stem cell biology are needed in the future.…”

Section: Discussionmentioning

confidence: 99%

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From embryonic development to human diseases: The functional role of caveolae/caveolin

Sohn

Brick

Tuan

2016

Birth Defects Research Pt C

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Caveolae, an almost ubiquitous, structural component of the plasma membrane, play a critical role in many functions essential for proper cell function, including membrane trafficking, signal transduction, extracellular matrix remodeling, and tissue regeneration. Three main types of caveolin proteins have been identified from caveolae since the discovery of caveolin-1 in the early 1990s. All three (Cav-1, Cav-2, and Cav-3) play crucial roles in mammalian physiology, and can effect pathogenesis in a wide range of human diseases. While many biological activities of caveolins have been uncovered since its discovery, their role and regulation in embryonic develop remain largely poorly understood, although there is increasing evidence that caveolins may be linked to lung and brain birth defects. Further investigations are clearly needed to decipher how caveolae/caveolins mediate cellular functions and activities of normal embryogenesis and how their perturbations contribute to developmental disorders.

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Section: Caveolae and Caveolins In Human Diseasementioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

From embryonic development to human diseases: The functional role of caveolae/caveolin

Sohn

Brick

Tuan

2016

Birth Defects Research Pt C

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“…These results indicate that there are significant compensatory interactions between eNOS and CAV1 in diabetes in vivo and that EDH is involved in coronary vasodilatation after ischemia [122]. Nevertheless, excessive NO production can induce superoxide release and nitrooxidative stress and consequently counter NO bioavailability [123]. Hence, CAV1 can possibly maintain the endothelial function through its ability to regulate NO production under normal physiological conditions.…”

Section: Role Of Caveolin-1 In Oxidative Stressmentioning

confidence: 89%

Role of Caveolin-1 in Diabetes and Its Complications

Haddad

Madhoun

Nizam

et al. 2020

Oxidative Medicine and Cellular Longevity

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It is estimated that in 2017 there were 451 million people with diabetes worldwide. These figures are expected to increase to 693 million by 2045; thus, innovative preventative programs and treatments are a necessity to fight this escalating pandemic disorder. Caveolin-1 (CAV1), an integral membrane protein, is the principal component of caveolae in membranes and is involved in multiple cellular functions such as endocytosis, cholesterol homeostasis, signal transduction, and mechanoprotection. Previous studies demonstrated that CAV1 is critical for insulin receptor-mediated signaling, insulin secretion, and potentially the development of insulin resistance. Here, we summarize the recent progress on the role of CAV1 in diabetes and diabetic complications.

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“…28 Under physiological conditions, caveolin-1 can bind to eNOS, leading to eNOS inhibition and reduced NO production. 29 eNOS binds to the caveolin-1 scaffolding domain and remains inactive when bound. When the interaction is disrupted, eNOS is active, which leads to increases in NO level and vascular permeability.…”

Section: Discussionmentioning

confidence: 99%

Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

Tang¹,

Jiang²,

He³

et al. 2018

IJN

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IntroductionNanomedicine has shown a great potential in perinatal medicine because of its characteristics of sustained, controlled release and targeting ability; on the other hand, it may also lead to unexpected toxicities such as embryotoxicity and even malformation after crossing the placental barrier, but data concerning transplacental transport are scarce. Pullulan acetate (PA) nanoparticles (NPs) are a promising nanocarrier derived from natural polysaccharide; however, their transplacental transport ability and mechanism are unknown.Materials and methodsIn this study, fluorescein isothiocyanate (FITC) conjugated PA (PA-FITC) was synthesized. PA-FITC NPs were characterized by dynamic light scattering, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The cytotoxicity of PA-FITC NPs at concentrations of 15, 30, 60, 125, 250, 500, 1,000 and 2,000 μg/mL was studied by cell counting kit-8. The human chorionic gonadotrophin (HCG) cytokine assay was conducted to evaluate the biological function of BeWo b30 cells. Endocytic mechanisms of PA-FITC NPs were investigated via fluorescence analysis. The monolayer properties were characterized by TEM, tight junction staining, transepithelial electrical resistance and fluorescein sodium transportation. The transport ability was measured in the cell based transwell model by confocal imaging and SEM.ResultsPA-FITC NPs were almost spherical shape with a size range of 200–300 nm. Cell viability of BeWo b30 cells was up to 100% in all groups. The concentrations of HCG increased with increasing numbers of cells and culture time, which showed the good biological function of BeWo b30 cells. PA-FITC NPs were rapidly endocytosed through caveolae-mediated endocytosis and pinocytosis, with uptake inhibition rates with nystatin (NY) and colchicines (Col) of 55% and 51% respectively. BeWo b30 cell monolayer was formed over 5 days. PA-FITC NPs were found in the cytoplasm of cells on the transwell membranes; while some NPs were found in the basolateral (fetal) compartment over 24 h.ConclusionIn summary, PA-FITC NPs are nontoxic, can cross the blood-placental barrier, and show mainly internalization to BeWo b30 cells through caveolae-mediated endocytosis and pinocytosis pathways, major via the former pathway. The results could benefit the adjustment and control of the transplacental transport of nanomedicines.

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Imbalance of Caveolin‐1 and eNOS Expression in the Pulmonary Vasculature of Experimental Diaphragmatic Hernia

Cited by 18 publications

References 36 publications

From embryonic development to human diseases: The functional role of caveolae/caveolin

From embryonic development to human diseases: The functional role of caveolae/caveolin

Role of Caveolin-1 in Diabetes and Its Complications

Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

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