Imbalance of angiotensin-converting enzymes affects myocardial apoptosis during cardiac arrest induced by acute pulmonary embolism in a porcine model

Abstract

Acute pulmonary embolism (APE) with cardiac arrest (CA) is associated with a high mortality rate. Even upon return of the spontaneous circulation (ROSC), APE-CA survivors are prone to myocardial cell apoptosis, a key cellular mechanism that induces heart failure. A recent study by our group discovered a post-resuscitation imbalance in the serum angiotensin-converting enzyme (ACE)2/ACE axis of the renin-angiotensin system (RAS), as well as regressive cardiac function in a porcine model of APE-CA. However, it has remained elusive how this imbalance in the ACE2/ACE axis affects myocardial cell apoptosis. In the present study, western blot and immunohistochemical analyses demonstrated that the RAS was only activated in the left myocardium, as evidenced by a decreased ACE2/ACE ratio following APE-CA and ROSC, but not the right myocardium. Ultrastructural analysis confirmed myocardial apoptosis in the left and right myocardium. Furthermore, B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax) and caspase-3 levels were elevated and Bcl-2 levels were decreased in the left myocardium following APE-CA and ROSC. Treatment with the ACE inhibitor captopril for 30 min after initiation of ROSC prevented the increase in Bax and the decrease in Bcl-2 in the left myocardium compared with that in saline-treated pigs. Captopril also inhibited the activation of extracellular signal-regulated kinase (ERK)1/2 in the left myocardium. The results of the present study suggest that an imbalance in the ACE2/ACE axis has an important role in myocardial apoptosis following APE-CA, which may be attributed to decreased ERK1/2 activation. In addition, it was indicated that captopril prevents apoptosis in the left myocardium after ROSC.

A comprehensive guide to the pharmacologic regulation of angiotensin converting enzyme 2 (ACE2), the SARS-CoV-2 entry receptor

“… Male Sprague-Dawley rats/ Focal cerebral ischemia model Increased brain ACE2 activity in controls and ischemic brains Tao et al (2018) Captopril/ ACE-Inh. Landrace pigs/ Acute pulmonary embolism model No change in cardiac ACE2 immunostaining and protein expression H. L. Xiao et al (2019) Enalapril/ ACE-Inh. Male Wistar albino rats/ Isoproterenol induced myocardial infarct model No change in cardiac ACE2 concentration in infarcted cardiac tissue Badae, El Naggar, and El Sayed (2019) Captopril/ ACE-Inh.…”

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