Imbalance Between Th17 Cells and Regulatory T Cells During Monophasic Experimental Autoimmune Uveitis

Wan, Fangzhu; Song, Jiuzhou; Tang, Kai; Zheng, Fengming; Guo, Dadong; Guo, Dadong; Bi, Hongsheng

doi:10.1007/s10753-015-0229-7

Cited by 22 publications

(18 citation statements)

References 27 publications

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“…Recent reports also suggest that its breakdown may play a key role in the pathogenesis of pars planitis, which is an idiopathic chronic intermediate uveitis [ 18 ]. Several immune alterations were previously described in experimental models of uveitis [ 20 , 21 ]. According to our knowledge, there is still a lack of reports on the immune status of pars planitis patients, and the present work is the first study performed in humans.…”

Section: Discussionmentioning

confidence: 99%

Imbalance in PB IL-17-Secreting and Regulatory Cells in Pars Planitis Is Associated with Dysregulation of IFN-γ-Secreting Cells, Especially in Patients with Clinical Complications

Kosmaczewska

Przeździecka-Dołyk

Turno-Kręcicka

et al. 2020

Mediators of Inflammation

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Background/Purpose. Pars planitis (PP) is a chronic intermediate uveitis of undefined cause, although autoimmune etiology is strongly suggested. This study was undertaken to evaluate circulating pro- and anti-inflammatory lymphocyte subpopulations in patients with PP. A better understanding of immune alterations in PP may contribute to the development of an appropriate biologic treatment approach. Material and Methods. In our case-control study, samples of peripheral blood from 15 patients with pars planitis and 17 healthy subjects were collected. The patients underwent a full ophthalmological evaluation including macular optical coherence tomography (OCT) and submacular choroid (with enhanced deep imaging) with the SD-OCT Spectralis system. By flow cytometry, we assessed lymphocyte subpopulations as follows: Th1 subset: CD3+CD8-IFN-γ+ and CCR4-CXCR3+IFN-γ+ cells, Th17 subset: CD3+CD8-IL-17+ and CCR4+CCR6+IL-17+ cells, T regulatory (Treg): CD4+CD25hiCD127- cells, T suppressor (Tsup): CD8+CD28-FOXP3+ cells, and B regulatory (Breg): CD19+CD24hiCD38hi cells. The results are presented as the median percentage of immune cells in comparison to those obtained from the healthy control group. Results. In patients, an increase in the population of Th17-secreting cells negatively correlated with the abundance of both IFN-γ-producing and T regulatory as well as suppressor cells, regarding all the phenotypes studied. Although a strong dependence of the PB Th1 cell compartment on the duration of the disease was observed, it was limited to the subgroup of patients with macular edema only. The frequency of B regulatory cells was unchanged compared to controls. Conclusions. In pars planitis, the alterations in lymphocyte cell distribution affect primarily the T cell repertoire. The imbalance in PB Th1/Th17/Treg cells creates proinflammatory conditions, strengthening the suggestion that the immune background may play a role in pars planitis pathogenesis. Also, circulating Th1 level may be of potential clinical relevance in terms of prediction of a more severe course of the disease.

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Section: Discussionmentioning

confidence: 99%

Imbalance in PB IL-17-Secreting and Regulatory Cells in Pars Planitis Is Associated with Dysregulation of IFN-γ-Secreting Cells, Especially in Patients with Clinical Complications

Kosmaczewska

Przeździecka-Dołyk

Turno-Kręcicka

et al. 2020

Mediators of Inflammation

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“…All samples were analyzed using an Applied Biosystems ABI 7500 Real-time PCR (USA) system. PCR conditions for gene amplification were as follows: 2 min at 50 °C, followed by 40 cycles of 95 °C for 2 min, 95 °C for 15 s, and 60 °C for 30 s) [ 18 ]. The PCR products were analyzed by using a basic relative quantification method, and the amplification products were confirmed based on the observation of a single peak in the melting curve.…”

Section: Methodsmentioning

confidence: 99%

The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model

Xie

Zhong

Liu

et al. 2017

BMC Complement Altern Med

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BackgroundConventional scientific studies had supported the use of polysaccharides and β-glucans from a number of fungi, including Ganoderma lucidum for the treatment of recurrent oral ulceration (ROU). Our aim of the present study was to evaluate whether freeze-dried powder from G. lucidum mycelia (FDPGLM) prevents ROU in rats.MethodsA Sprague-Dawley (SD) rat model with ROU was established by autoantigen injection. The ROU rats were treated with three different dosages of FDPGLM and prednisone acetate (PA), and their effects were evaluated according to the clinical therapeutic evaluation indices of ROU.ResultsHigh-dose FDPGLM induced significantly prolonged total intervals and a reduction in the number of ulcers and ulcer areas, thereby indicating that the treatment was effective in preventing ROU. Enzyme-linked immunosorbent assay (ELISA) showed that high-dose FDPGLM significantly enhanced the serum transforming growth factor-β1 (TGF-β1) levels, whereas reduced those of interleukin-6 (IL-6) and interleukin-17 (IL-17). Flow cytometry (FCM) showed that the proportion of CD4+ CD25+ Foxp3+ (forkhead box P3) regulatory T cells (Tregs) significantly increased by 1.5-fold in the high-dose FDPGLM group compared to that in the rat model group (P < 0.01). The application of middle- and high-dose FDPGLM also resulted in the upregulation of Foxp3 and downregulation of retinoid-related orphan receptor gamma t(RORγt) mRNA.ConclusionHigh-dose FDPGLM possibly plays a role in ROU by promoting CD4+ CD25+ Foxp3+ Treg and inhibiting T helper cell 17 differentiation. This study also shows that FDPGLM may be potentially used as a complementary and alternative medicine treatment scheme for ROU.Electronic supplementary materialThe online version of this article (10.1186/s12906-017-2021-8) contains supplementary material, which is available to authorized users.

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“…The imbalance ratio of Treg and helper T lymphocytes 17 (Th17) will affect the progress of interactions and is related to the occurrence and development of sepsis 21 and other diseases 22,23 .The results of flow cytometry manifested a high ratio of Th17 /Treg in sepsis murine.Both ETDCs and miR155 -/-ETDCs infusion displayed a ability to attenuate Th17/Treg ratio while the Th17/Treg ratio was lowest in the miR155 -/-ETDCs treatment group.…”

Section: Infusion Of Etdcs and Mir155 -/-Etdcs Decreased The Th17/trementioning

confidence: 99%

Silencing microRNA155 transforms the properties of endotoxin tolerant dendritic cells and enhance its therapeutic effect on sepsis murine model.

Hou

Yang

et al. 2020

Preprint

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Sepsis refers to the body's dysfunctional response to infection and leads to life-threatening organ dysfunction. Dentritic cells (DCs), a kind of powerful functional antigen-presenting cell, plays a critical role in immune response and has been used in the field of immunotherapy for multiple diseases. Endotoxin tolerance is a phenomenon that believed to prevent the organism from producing persistent and excessive inflammatory reaction and numerous studies indicated that endotoxin tolerant dendritic cells (ETDCs) have therapeutic effects on experimental models of several diseases. MicroRNA155(miR155), which plays a pivotal role in various physiological and pathological processes in immunity, was reported associated with the formation of endotoxin tolerance. In this paper, we explored the change of properties after silencing miR155 in ETDCs then attempted to study its effects on the murine model of sepsis. Endotoxin tolerant dendritic cells were transfected with adenovirus silencing miR155 and negative control adenovirus, thereafter collected for reverse transcription polymerase chain reaction of miR155, costimulatory molecules analysis, allogeneic mixed lymphocyte reaction and cytokine concentration evaluation. 42 balb/c mice were randomly divided into control group, sham operation group, sepsis group, ETDCs treatment group and miR155-/-ETDCs treatment group. Two treatment groups respectively received tail vein injection of ETDCs and miR155-/-ETDCs 24h earlier than cacal ligation puncture. Our results revealed that silencing miR155 could deepen the immature status of ETDCs and both ETDCs and miR155-/-ETDCs performed a protective effect in murine sepsis models while miR155-/-ETDCs got a better protection. We reasoned that miR155 participates the formation of endotoxin tolerance and the protective effects of ETDCs and miR155-/-ETDCs in the early stage of sepsis may achieved by altering the concentration of mouse cytokines to affect T cell differentiation and exert negative immune regulation.

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Imbalance Between Th17 Cells and Regulatory T Cells During Monophasic Experimental Autoimmune Uveitis

Cited by 22 publications

References 27 publications

Imbalance in PB IL-17-Secreting and Regulatory Cells in Pars Planitis Is Associated with Dysregulation of IFN-γ-Secreting Cells, Especially in Patients with Clinical Complications

Imbalance in PB IL-17-Secreting and Regulatory Cells in Pars Planitis Is Associated with Dysregulation of IFN-γ-Secreting Cells, Especially in Patients with Clinical Complications

The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model

Silencing microRNA155 transforms the properties of endotoxin tolerant dendritic cells and enhance its therapeutic effect on sepsis murine model.

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