Imatinib mesylate inhibits in vitro and ex vivo biological responses related to vascular occlusion in giant cell arteritis

Lozano, Ester; Segarra, Marta; García‐Martínez, Ana; Hernández‐Rodríguez, José; Cid, María C.

doi:10.1136/ard.2007.070805

Cited by 71 publications

(66 citation statements)

References 38 publications

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“…30 Concordant with our results, a recent study in zebrafish has reported the involvement of SOX11b, the human ortholog of SOX11, in promoting sprouting angiogenesis of caudal vein plexus. 31 The gene transcriptional profile of SOX11-positive MCL cell lines was enriched in signatures related to angiogenesis and expressed higher levels of several proangiogenic factors than SOX11-negative ones.…”

Section: Discussionsupporting

confidence: 93%

SOX11 promotes tumor angiogenesis through transcriptional regulation of PDGFA in mantle cell lymphoma

Palomero¹,

Vegliante²,

Rodríguez³

et al. 2014

Blood

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Section: Discussionsupporting

confidence: 93%

SOX11 promotes tumor angiogenesis through transcriptional regulation of PDGFA in mantle cell lymphoma

Palomero¹,

Vegliante²,

Rodríguez³

et al. 2014

Blood

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“…However, it is important to remark that inflammatory cells may not be the only source of cytokines. We have previously shown that cultured myointimal cells derived from temporal arteries are able to produce substantial amounts of IL-6 (23,24). Therefore, both remaining inflammatory cells and regenerating smooth muscle cells may contribute to proinflammatory cytokine production, particularly IL-6.…”

Section: Discussionmentioning

confidence: 99%

Clinical relevance of persistently elevated circulating cytokines (tumor necrosis factor α and interleukin‐6) in the long‐term followup of patients with giant cell arteritis

García‐Martínez¹,

Hernández‐Rodríguez²,

Espígol‐Frigolé³

et al. 2010

Arthritis Care & Research

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Objective. To assess the clinical relevance of increased circulating cytokines in patients with giant cell arteritis (GCA) after long-term followup. Methods. We performed a cross-sectional evaluation of 54 patients with biopsy-proven GCA prospectively followed for a median of 5.4 years (range 4 -10.5 years). GCA-related complications, vascular events, relapses, current prednisone dose, time required to achieve a maintenance prednisone dosage <10 mg/day, cumulated prednisone at that point, and adverse effects during followup were recorded. Serum interleukin-6 (IL-6) and tumor necrosis factor ␣ (TNF␣) were determined by immunoassay. Results. All patients were in clinical remission. Both cytokines were significantly higher in patients than in controls (mean ؎ SD 21 ؎ 35 versus 5 ؎ 11 pg/ml; P < 0.001 for IL-6 and mean ؎ SD 32 ؎ 14 versus 16 ؎ 9 pg/ml; P < 0.001 for TNF␣). No differences were found in patients with or without GCA-related complications or vascular events during followup. Circulating cytokines were significantly higher in patients who had experienced relapses (mean ؎ SD 25 ؎ 39 versus 10 ؎ 11 pg/ml; P ‫؍‬ 0.04 for IL-6 and mean ؎ SD 34 ؎ 15 versus 25 ؎ 11 pg/ml; P ‫؍‬ 0.042 for TNF␣). IL-6 was significantly higher in patients still requiring prednisone (mean ؎ SD 29 ؎ 45 versus 13 ؎ 17 pg/ml; P ‫؍‬ 0.008), and TNF␣ correlated with cumulated prednisone dose (r ‫؍‬ 0.292, P ‫؍‬ 0.04). No significant relationship was found between elevated cytokines and prednisone adverse effects or patients' quality of life. Conclusion. Circulating TNF␣ and IL-6 may persist elevated in GCA patients after long-term followup and remain higher in patients who have experienced more relapsing disease. However, in this patient cohort, elevated circulating cytokines were not associated with increased frequency of GCA complications, vascular events, or treatment-related side effects.

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“…These explant cultures can be further used as ex vivo assays for factors important in angiogenesis as well as for understanding vessel disease processes. Another use of this explant culture on 3D basement membrane, is to isolate the cells that grow out from the explants (Lozano et al, 2008). Human temporal artery explants in 3D basement membrane culture are a source of myointimal cells.…”

Section: Isolated Endothelial Cells and Vessel Outgrowth From Explantsmentioning

confidence: 99%

Advancing science and technology via 3D culture on basement membrane matrix

Benton

George

Kleinman

et al. 2009

Journal Cellular Physiology

124

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Many cells in tissues are in contact with a highly specialized extracellular matrix, termed the basement membrane. Basement membranes have certain common components, including collagen IV, laminins, heparan sulfate proteoglycans, and growth factors which have a wide variety of biological activities. Extracts of basement membrane-rich tissue have yielded material suitable for studying cell-basement membrane interactions. Cells cultured in a 3D basement membrane matrix allow the in vitro modeling of cell behavior, including differentiation, apoptosis, steps in capillary formation, cancer growth, invasion, etc. It has also led to the development of widely used assays for invasion and angiogenesis and more recently for tumor cell dormancy. Importantly, stem cell culture in 3D basement membrane matrices has provided important advances that allow for expansion of these cells in feeder layer-free cultures and for studying their differentiation. 3D basement membrane culture has allowed the molecular dissection of pathways and genes important in differentiation, aided in the identification of progenitor cells, and led to the development of tissue constructs which may be models for regenerative medicine. This review will outline how this technology has led to important research assays and findings that have advanced our understanding of tissue development and disease and aided in the preclinical development of various therapeutics.

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Imatinib mesylate inhibits in vitro and ex vivo biological responses related to vascular occlusion in giant cell arteritis

Cited by 71 publications

References 38 publications

SOX11 promotes tumor angiogenesis through transcriptional regulation of PDGFA in mantle cell lymphoma

SOX11 promotes tumor angiogenesis through transcriptional regulation of PDGFA in mantle cell lymphoma

Clinical relevance of persistently elevated circulating cytokines (tumor necrosis factor α and interleukin‐6) in the long‐term followup of patients with giant cell arteritis

Advancing science and technology via 3D culture on basement membrane matrix

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