Imatinib decreases endometrial stromal cell transmesothelial migration and proliferation in the extracellular matrix of modeled peritoneum

Griffith, Jason; Binkley, Peter; Kirma, Nameer B.; Schenken, Robert S.; Tekmal, RR; Witz, Craig A.

doi:10.1016/j.fertnstert.2007.07.208

Cited by 1 publication

(1 citation statement)

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“…These findings were replicated using not only ESCs from patients but also the immortalized cell line THESC from Yale. 58 It appears from the above studies that replicating the basement membrane matrix with either Matrigel or collagen matrices allows for a more complete model to investigate both invasion and proliferation. Three-dimensional models may better simulate processes occurring in vivo and also for functional assessment of the individual cells based on structural integrity of the experimental endometriotic implant.…”

Section: Invasion Chambersmentioning

confidence: 99%

In Vitro Models to Study the Pathogenesis of Endometriosis

2010

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Several in vitro models that attempt to replicate the intraperitoneal environment have been developed to study the pathogenesis of endometriosis. The chicken chorioallantotic membrane has been used, but it has not been well characterized and may introduce some species specific variables. In vitro models using human tissues include amniotic membrane, human peritoneal explants, and cell culture monolayers. These models have been used to qualitatively, quantitatively, and temporally assess attachment of endometrial cells to peritoneal mesothelial and subsequent transmesothelial invasion. These models have also been used to assess the role of cytokines in the development of the early endometriotic lesion. Two- and three dimensional invasion chamber models have been utilized to assess endometrial cell interactions with peritoneal mesothelial cells and the extracellular matrix. Invasion models are also useful to evaluate novel therapeutic approaches. This review will focus on the above models to assist reproductive scientists interested in the pathogenesis of endometriosis.

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Section: Invasion Chambersmentioning

confidence: 99%