Imaging with second-harmonic radiation probes in living tissue

Grange, Rachel; Lanvin, Thomas; Hsieh, Chia-Lung; Pu, Ye; Psaltis, Demetri

doi:10.1364/boe.2.002532

Cited by 63 publications

(60 citation statements)

References 28 publications

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“…SHG has been proven to be a highly useful technology for the non-invasive intravital imaging of the collagenrich microenvironment during tumor invasion (Wang et al, 2002), of muscle defects in the mouse ear and of zebrafish embryos (Ramspacher et al, 2015). Furthermore, nanoparticles generating second harmonics have been used in vivo and represent new imaging tools (Grange et al, 2011;Pantazis et al, 2010).…”

Section: Label-free Intravital Imagingmentioning

confidence: 99%

Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology

Follain

Mercier

Osmani

et al. 2016

Journal of Cell Science

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Life is driven by a set of biological events that are naturally dynamic and tightly orchestrated from the single molecule to entire organisms. Although biochemistry and molecular biology have been essential in deciphering signaling at a cellular and organismal level, biological imaging has been instrumental for unraveling life processes across multiple scales. Imaging methods have considerably improved over the past decades and now allow to grasp the inner workings of proteins, organelles, cells, organs and whole organisms. Not only do they allow us to visualize these events in their most-relevant context but also to accurately quantify underlying biomechanical features and, so, provide essential information for their understanding. In this Commentary, we review a palette of imaging (and biophysical) methods that are available to the scientific community for elucidating a wide array of biological events. We cover the most-recent developments in intravital imaging, light-sheet microscopy, superresolution imaging, and correlative light and electron microscopy. In addition, we illustrate how these technologies have led to important insights in cell biology, from the molecular to the whole-organism resolution. Altogether, this review offers a snapshot of the current and state-of-the-art imaging methods that will contribute to the understanding of life and disease.

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Section: Label-free Intravital Imagingmentioning

confidence: 99%

Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology

Follain

Mercier

Osmani

et al. 2016

Journal of Cell Science

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“…This enabled the team of Yuste and Eisenthal to measure the length of the dendritic spine necks in living neurons and to determine that dendritic spines linearize the summation of excitatory potentials [38]. In another promising approach, SHG active surface modifiable non-centrosymmetric nanodoublers [39] have been introduced as a practical alternative to fluorescent molecules and quantum dots, to track processes through in-vitro (cell) imaging [40][41][42][43][44]. These studies clearly demonstrate the unique ability and promising possibilities of SHG microscopy to probe materials and interfaces, identify and quantify histoarchitectural tissue alterations, and to track changes in living systems.…”

Section: Introductionmentioning

confidence: 99%

High throughput second harmonic imaging for label-free biological applications

Macias-Romero¹,

Didier²,

Jourdain³

et al. 2014

Opt. Express

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Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO 3 nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture. ©2014 Optical Society of America

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“…Tests will entail adding a fluorescing Ca 2+ indicator to the flow solution and conducting laser imaging during flow through experiments to capture Ca 2+ release during dissolution. SPIFI has been used with TPEF and with second-harmonic generation (SHG) to image inorganic and biological samples [6,7]. However, this would be the first time in-situ geochemical dissolution has been captured with SPIFI.…”

mentioning

confidence: 99%

Analysis of Anorthite Dissolution on the Submicrometer Scale

et al. 2018

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Dissolution reactions are critical to understanding various earth science and engineering processes: the release of contaminants into potentially potable groundwater, fluvial morphology development, and integrity of engineered structures such as well bores, dams and building foundations [1][2][3]. Yet there exist pervasive discrepancies between laboratory and field determined reaction rates [4]. Relationships between dissolution reactions across these scales can be described using Damköhler numbers, or ratios between mass transfer and chemical reaction rates (Eq. 1). This research investigates how variations in pH and Darcy flux (U x ) affect the reaction rate (R) and flow velocity respectively, during anorthite dissolution between the effective and local scale dissolution (Eq. 1). In situ analyses of anorthite dissolution (Eq. 2) focus on changes in solution chemistry at the effective area scale -the pore network -and at the local area scale -the interpore.Flow experiments are conducted by injecting a fluid into 65 x 25 m channels laser etched into a sample of anorthite [5] (Fig. 1). The idealized pore network is sealed with a clear layer of polydimethylsiloxane (PDMS), allowing fluid flow between the PDMS layer and the channel bottoms. An acidic solution (pH ~ 3.01) is injected at one end of the channel network and fluid samples are collected at the outlet after having reacted with the anorthite channel walls. Effective reaction rates are calculated from the difference in Ca 2+ , Al 3+ , SO 2(aq) , and H + concentrations between inlet and outlet fluids. Flow rate is determined from time-lapse images of 2µm diameter Fluoro-Max Red fluorescent beads using an Olympus BX51 fluorescence microscope with a 0.02 sec exposure time.To measure reactions on the local or inter-pore scale, we must capture information on micron to nanometer scale processes. Using two-photon excitation fluorescence imaging (TPEF) with a ytterbium femtosecond laser developed at Colorado School of Mines, spatial frequency modulation for imaging (SPIFI) will be tested to detect Ca 2+ release on the micron to submicron scale. Tests will entail adding a fluorescing Ca 2+ indicator to the flow solution and conducting laser imaging during flow through experiments to capture Ca 2+ release during dissolution. SPIFI has been used with TPEF and with second-harmonic generation (SHG) to image inorganic and biological samples [6,7]. However, this would be the first time in-situ geochemical dissolution has been captured with SPIFI.Electron microscopy is used to characterize and select appropriate mineral samples (ESEM and EDS), map regions of potential sample damage during laser ablation for subsequent removal (TEM) (Fig. 2), and elemental mapping of channel cross sections before and after flow through experiments (STEM).Anorthite specimen with etched flow-through channels have been used to induce and capture effective mineral dissolution. Continued work will involve varying the acidity and velocity of flow-through solutions to assess the dependence of ano...

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Imaging with second-harmonic radiation probes in living tissue

Cited by 63 publications

References 28 publications

Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology

Seeing is believing: multi-scale spatio-temporal imaging towards in vivo cell biology

High throughput second harmonic imaging for label-free biological applications

Analysis of Anorthite Dissolution on the Submicrometer Scale

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